Human beta-defensin DEFB126 is capable of inhibiting LPS-mediated inflammation

Applied Microbiology and Biotechnology

Volumn 97, Issue 8, 2013, Pages 3395-3408

  Liu, Haiyan ^a   Yu, Heguo ^b   Gu, Yihua ^b   Xin, Aijie ^b   Zhang, Yonglian ^b,c   Diao, Hua ^b   Lin, Donghai ^a,d  

^a CHINA PHARMACEUTICAL UNIVERSITY   (China)

^b SHANGHAI INSTITUTE OF PLANNED PARENTHOOD RESEARCH   (China)

^c INSTITUTE OF BIOCHEMISTRY AND CELL BIOLOGY   (China)

^d XIAMEN UNIVERSITY   (China)

Author keywords

Anti inflammatory; Cytokines; Defensin 126; LPS; RAW264.7

Indexed keywords

ANTI-INFLAMMATORIES; CYTOKINES; DEFENSINS; LPS; RAW264.7;

CELL CULTURE; MICROORGANISMS; PATHOLOGY;

PEPTIDES;

ANTIINFLAMMATORY AGENT; BETA DEFENSIN; BETA DEFENSIN 126; INTERLEUKIN 1ALPHA; INTERLEUKIN 1BETA; INTERLEUKIN 6; LIPOPOLYSACCHARIDE; MESSENGER RNA; MITOGEN ACTIVATED PROTEIN KINASE; PROTEIN P42; PROTEIN P44; SYNAPTOPHYSIN; TUMOR NECROSIS FACTOR ALPHA; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; BIOCHEMISTRY; CHEMICAL BINDING; CYTOLOGY; FUNCTIONAL ROLE; HOMINID; IMMUNITY; MEDICINE; NEUTRALIZATION; PEPTIDE; POLYSACCHARIDE; PROTEIN; REPRODUCTIVE BIOLOGY; SECRETION;

ANIMAL CELL; ANTIINFLAMMATORY ACTIVITY; ANTIMICROBIAL ACTIVITY; ARTICLE; BINDING AFFINITY; CONTROLLED STUDY; CYTOKINE RELEASE; DOWN REGULATION; DRUG LABELING; DRUG POTENCY; DRUG PROTEIN BINDING; IN VITRO STUDY; INFLAMMATION; MACROPHAGE; NONHUMAN; PROTEIN EXPRESSION; PROTEIN SECONDARY STRUCTURE; SIGNAL TRANSDUCTION;

ANIMALS; BETA-DEFENSINS; CELL LINE; CYTOKINES; DOWN-REGULATION; HUMANS; IMMUNOLOGIC FACTORS; INFLAMMATION; LIPOPOLYSACCHARIDES; MACROPHAGES; MICE; NEUTRALIZATION TESTS; PROTEIN BINDING;

MURINAE;

EID: 84876671522     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4588-9     Document Type: Article

References (48)

1. Andra J, Gutsmann T, Garidel P, Brandenburg K (2006) Mechanisms of endotoxin neutralization by synthetic cationic compounds. J Endotoxin Res 12(5):261-277
2. Bhattacharjya S (2010) De novo designed lipopolysaccharide binding peptides: structure based development of antiendotoxic and antimicrobial drugs. Curr Med Chem 17(27):3080-3093
3. Brogden KA, Heidari M, Sacco RE, Palmquist D, Guthmiller JM, Johnson GK, Jia HP, Tack BF, McCray PB (2003) Defensin-induced adaptive immunity in mice and its potential in preventing periodontal disease. Oral Microbiol Immunol 18(2):95-99
4. Cao D, Li Y, Yang R, Wang Y, Zhou Y, Diao H, Zhao Y, Zhang Y, Lu J (2010) Lipopolysaccharide-induced epididymitis disrupts epididymal beta-defensin expression and inhibits sperm motility in rats. Biol Reprod 83(6):1064-1070
5. Casallanovo F, de Oliveira FJ, de Souza FC, Ros U, Martinez Y, Penton D, Tejuca M, Martinez D, Pazos F, Pertinhez TA, Spisni A, Cilli EM, Lanio ME, Alvarez C, Schreier S (2006) Model peptides mimic the structure and function of the N-terminus of the pore-forming toxin sticholysin II. Biopolymers 84(2):169-180
6. Corrales-Garcia LL, Possani LD, Corzo G (2010) Expression systems of human beta-defensins: vectors, purification and biological activities. Amino Acids 40(1):5-13
7. de Haas CJ, Haas PJ, van Kessel KP, van Strijp JA (1998) Affinities of different proteins and peptides for lipopolysaccharide as determined by biosensor technology. Biochem Biophys Res Commun 252(2):492-496
8. Diao H, Guo C, Lin D, Zhang Y (2007) Intein-mediated expression is an effective approach in the study of beta-defensins. Biochem Biophys Res Commun 357(4):840-846
9. Diao H, Yu HG, Sun F, Zhang YL, Tanphaichitr N (2010) Rat recombinant beta-defensin 22 is a heparin-binding protein with antimicrobial activity. Asian J Androl 13(2):305-311
10. Dong J, Yu H, Zhang Y, Diao H, Lin D (2011) Soluble fusion expression and characterization of human beta-defensin 3 using a novel approach. Protein Pept Lett 18(11):1126-1132
11. Durr UH, Sudheendra US, Ramamoorthy A (2006) LL-37, the only human member of the cathelicidin family of antimicrobial peptides. Biochim Biophys Acta 1758(9):1408-1425
12. Fellermann K, Stange DE, Schaeffeler E, Schmalzl H, Wehkamp J, Bevins CL, Reinisch W, Teml A, Schwab M, Lichter P, Radlwimmer B, Stange EF (2006) A chromosome 8 gene-cluster polymorphism with low human beta-defensin 2 gene copy number predisposes to Crohn disease of the colon. Am J Hum Genet 79(3):439-448
13. Garcia JR, Jaumann F, Schulz S, Krause A, Rodriguez-Jimenez J, Forssmann U, Adermann K, Kluver E, Vogelmeier C, Becker D, Hedrich R, Forssmann WG, Bals R (2001) Identification of a novel, multifunctional beta-defensin (human beta-defensin 3) with specific antimicrobial activity. Its interaction with plasma membranes of Xenopus oocytes and the induction of macrophage chemoattraction. Cell Tissue Res 306(2):257-264
14. Hall SH, Yenugu S, Radhakrishnan Y, Avellar MC, Petrusz P, French FS (2007) Characterization and functions of beta defensins in the epididymis. Asian J Androl 9(4):453-462
15. Hirschfeld M, Ma Y, Weis JH, Vogel SN, Weis JJ (2000) Cutting edge: repurification of lipopolysaccharide eliminates signaling through both human and murine toll-like receptor 2. J Immunol 165(2):618-622
16. Hollox EJ, Huffmeier U, Zeeuwen PL, Palla R, Lascorz J, Rodijk-Olthuis D, van de Kerkhof PC, Traupe H, de Jongh G, den Heijer M, Reis A, Armour JA, Schalkwijk J (2008) Psoriasis is associated with increased beta-defensin genomic copy number. Nat Genet 40(1):23-25
17. Huang L, Leong SS, Jiang R (2009) Soluble fusion expression and characterization of bioactive human beta-defensin 26 and 27. Appl Microbiol Biotechnol 84(2):301-308
18. Julenius K, Molgaard A, Gupta R, Brunak S (2005) Prediction, conservation analysis, and structural characterization of mammalian mucin-type O-glycosylation sites. Glycobiology 15(2):153-164
19. Kagan BL, Ganz T, Lehrer RI (1994) Defensins: a family of antimicrobial and cytotoxic peptides. Toxicology 87(1-3):131-149
20. Larrick JW, Hirata M, Balint RF, Lee J, Zhong J, Wright SC (1995) Human CAP18: a novel antimicrobial lipopolysaccharide-binding protein. Infect Immun 63(4):1291-1297
21. Lehrer RI (2004) Primate defensins. Nat Rev Microbiol 2(9):727-738
22. Lund-Katz S, Nguyen D, Dhanasekaran P, Kono M, Nickel M, Saito H, Phillips MC (2010) Surface plasmon resonance analysis of the mechanism of binding of apoA-I to high density lipoprotein particles. J Lipid Res 51(3):606-617
23. Miller SI, Ernst RK, Bader MW (2005) LPS, TLR4 and infectious disease diversity. Nat Rev Microbiol 3(1):36-46
24. Motzkus D, Schulz-Maronde S, Heitland A, Schulz A, Forssmann WG, Jubner M, Maronde E (2006) The novel beta-defensin DEFB123 prevents lipopolysaccharide-mediated effects in vitro and in vivo. FASEB J 20(10):1701-1702
25. Ng PM, Jin Z, Tan SS, Ho B, Ding JL (2004) C-reactive protein: a predominant LPS-binding acute phase protein responsive to Pseudomonas infection. J Endotoxin Res 10(3):163-174
26. Niyonsaba F, Iwabuchi K, Matsuda H, Ogawa H, Nagaoka I (2002) Epithelial cell-derived human beta-defensin-2 acts as a chemotaxin for mast cells through a pertussis toxin-sensitive and phospholipase C-dependent pathway. Int Immunol 14(4):421-426
27. Patil AA, Cai Y, Sang Y, Blecha F, Zhang G (2005) Cross-species analysis of the mammalian beta-defensin gene family: presence of syntenic gene clusters and preferential expression in the male reproductive tract. Physiol Genom 23(1):5-17
28. Pazgier M, Hoover DM, Yang D, Lu W, Lubkowski J (2006) Human beta-defensins. Cell Mol Life Sci 63(11):1294-1313
29. Rhule A, Navarro S, Smith JR, Shepherd DM (2006) Panax notoginseng attenuates LPS-induced pro-inflammatory mediators in RAW264.7 cells. J Ethnopharmacol 106(1):121-128
30. Rieg S, Meier B, Fahnrich E, Huth A, Wagner D, Kern WV, Kalbacher H (2010) Differential activity of innate defense antimicrobial peptides against Nocardia species. BMC Microbiol 10:61
31. Rosenfeld Y, Papo N, Shai Y (2006) Endotoxin (lipopolysaccharide) neutralization by innate immunity host-defense peptides. Peptide properties and plausible modes of action. J Biol Chem 281(3):1636-1643
32. Schagger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 166(2):368-379
33. Schumann RR, Leong SR, Flaggs GW, Gray PW, Wright SD, Mathison JC, Tobias PS, Ulevitch RJ (1990) Structure and function of lipopolysaccharide binding protein. Science 249(4975):1429-1431
34. Schutte BC, Mitros JP, Bartlett JA, Walters JD, Jia HP, Welsh MJ, Casavant TL, McCray PB Jr (2002) Discovery of five conserved beta-defensin gene clusters using a computational search strategy. Proc Natl Acad Sci USA 99(4):2129-2133
35. Scott MG, Vreugdenhil AC, Buurman WA, Hancock RE, Gold MR (2000) Cutting edge: cationic antimicrobial peptides block the binding of lipopolysaccharide (LPS) to LPS binding protein. J Immunol 164(2):549-553
36. Semple F, Webb S, Li HN, Patel HB, Perretti M, Jackson IJ, Gray M, Davidson DJ, Dorin JR (2010) Human beta-defensin 3 has immunosuppressive activity in vitro and in vivo. Eur J Immunol 40(4):1073-1078
37. Semple F, MacPherson H, Webb S, Cox SL, Mallin LJ, Tyrrell C, Grimes GR, Semple CA, Nix MA, Millhauser GL, Dorin JR (2011) Human beta-defensin 3 affects the activity of pro-inflammatory pathways associated with MyD88 and TRIF. Eur J Immunol 41(11):3291-3300
38. Suttles J, Carruth LM, Mizel SB (1990) Detection of IL-1 alpha and IL-1 beta in the supernatants of paraformaldehyde-treated human monocytes. Evidence against a membrane form of IL-1. J Immunol 144(1):170-174
39. Svinarich DM, Wolf NA, Gomez R, Gonik B, Romero R (1997) Detection of human defensin 5 in reproductive tissues. Am J Obstet Gynecol 176(2):470-475
40. Tollner TL, Yudin AI, Tarantal AF, Treece CA, Overstreet JW, Cherr GN (2008) Beta-defensin 126 on the surface of macaque sperm mediates attachment of sperm to oviductal epithelia. Biol Reprod 78(3):400-412
41. Tollner TL, Venners SA, Hollox EJ, Yudin AI, Liu X, Tang G, Xing H, Kays RJ, Lau T, Overstreet JW, Xu X, Bevins CL, Cherr GN (2011) A common mutation in the defensin DEFB126 causes impaired sperm function and subfertility. Sci Transl Med 3(92):92ra65
42. Tollner TL, Bevins CL, Cherr GN (2012) Multifunctional glycoprotein DEFB126 - a curious story of defensin-clad spermatozoa. Nat Rev Urol 9(7):365-375
43. Wira CR, Fahey JV, Sentman CL, Pioli PA, Shen L (2005) Innate and adaptive immunity in female genital tract: cellular responses and interactions. Immunol Rev 206:306-335
44. Xu Z, Huang CX, Li Y, Wang PZ, Ren GL, Chen CS, Shang FJ, Zhang Y, Liu QQ, Jia ZS, Nie QH, Sun YT, Bai XF (2007) Toll-like receptor 4 siRNA attenuates LPS-induced secretion of inflammatory cytokines and chemokines by macrophages. J Infect 55(1):e1-e9
45. Yudin AI, Generao SE, Tollner TL, Treece CA, Overstreet JW, Cherr GN (2005) Beta-defensin 126 on the cell surface protects sperm from immunorecognition and binding of anti-sperm antibodies. Biol Reprod 73(6):1243-1252
46. Zanoni I, Granucci F (2010) Differences in lipopolysaccharide-induced signaling between conventional dendritic cells and macrophages. Immunobiology 215(9-10):709-712
47. Zhang W, Wei Q (2011) Calcineurin stimulates the expression of inflammatory factors in RAW 264.7 cells by interacting with proteasome subunit alpha type 6. Biochem Biophys Res Commun 407(4):668-673
48. Zhang M, Qiu Z, Li Y, Yang Y, Zhang Q, Xiang Q, Su Z, Huang Y (2012) Construction and characterization of a recombinant human beta defensin 2 fusion protein targeting the epidermal growth factor receptor: in vitro study. Appl Microbiol Biotechnol