Twelve-gel slide format optimised for comet assay and fluorescent in situ hybridisation

Toxicology Letters

Volumn 195, Issue 1, 2010, Pages 31-34

  Shaposhnikov, Sergey ^a   Azqueta, Amaya ^a   Henriksson, Sara ^b   Meier, Silja ^a,c   Gaivão, Isabel ^d   Huskisson, Neville H ^e   Smart, Andrew ^e   Brunborg, Gunnar ^c   Nilsson, Mats ^b   Collins, Andrew R ^a  

^a UNIVERSITY OF OSLO   (Norway)

^b UPPSALA UNIVERSITY   (Sweden)

^c NORWEGIAN INSTITUTE OF PUBLIC HEALTH   (Norway)

^d UNIVERSITY OF TRÁS OS MONTES AND ALTO DOURO   (Portugal)

^e Unit 2   (United Kingdom)

Author keywords

Comet assay; Fluorescent in situ hybridisation; High throughput method

Indexed keywords

HYDROGEN PEROXIDE; PHOTOSENSITIZING AGENT; PYRROLIDINE DERIVATIVE; QUINOLIZINE DERIVATIVE; RO 19 8022; RO 19-8022;

ARTICLE; BIOLOGICAL MONITORING; COMET ASSAY; CONCENTRATION RESPONSE; CONTROLLED STUDY; DNA DAMAGE; DNA PROBE; DNA REPAIR; EQUIPMENT DESIGN; FLUORESCENCE IN SITU HYBRIDIZATION; GENOTOXICITY; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; INTERMETHOD COMPARISON; MATERIALS TESTING; PRIORITY JOURNAL; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; TOXICITY TESTING; HELA CELL; INSTRUMENTATION; METHODOLOGY;

COMET ASSAY; DNA DAMAGE; HELA CELLS; HUMANS; IN SITU HYBRIDIZATION, FLUORESCENCE; PHOTOSENSITIZING AGENTS; PYRROLIDINES; QUINOLIZINES;

EID: 77951915631     PISSN: 03784274     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.toxlet.2010.02.017     Document Type: Article

References (7)

1. Collins A.R., Duthie S.J., Dobson V.L. Direct enzymic detection of endogenous oxidative base damage in human lymphocyte DNA. Carcinogenesis 1993, 14:1733-1735.
2. Collins A.R. The comet assay for DNA damage and repair: principles, applications and limitations. Molecular Biotechnology 2004, 26:249-261.
3. Dušinská M., Collins A.R. Detection of oxidised purines and UV induced photoproducts in DNA of single cells, by inclusion of lesion-specific enzymes in the comet assay. Alternatives to Laboratory Animals 1996, 24:405-411.
4. Larsson C., Koch J., Nygren A., Janssen G., Raap A.K., Landegren U., Nilsson M. In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes. Nature Methods 2004, 1:227-232.
5. Shaposhnikov S., Larsson C., Henriksson S., Collins A., Nilsson M. Detection of Alu sequences and mtDNA in comets using padlock probes. Mutagenesis 2006, 21:243-247.
6. Shaposhnikov S., Frengen F., Collins A.R. Increasing the resolution of the comet assay using fluorescent in situ hybridization-a review. Mutagenesis 2009, 4:383-389.
7. Shaposhnikov, S., Thomsen, P.D., Collins, A.R., in press. Combining fluorescent in situ hybridization with the comet assay for targeted examination of DNA damage and repair. In: Didenko, V. (Ed.), In situ Detection of DNA Damage. Humana Press.