메뉴 건너뛰기




Volumn 30, Issue 3, 2013, Pages 315-323

Substrates and supplements for hESCs: A critical review

Author keywords

Embryonic stem cells; hESC; Substrate; Supplements

Indexed keywords

ACTIVIN; COLLAGEN; FIBRONECTIN; LAMININ; LEUKEMIA INHIBITORY FACTOR; NOGGIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR NANOG; TRANSFORMING GROWTH FACTOR BETA;

EID: 84876292483     PISSN: 10580468     EISSN: 15737330     Source Type: Journal    
DOI: 10.1007/s10815-012-9914-8     Document Type: Article
Times cited : (11)

References (80)
  • 1
    • 0034669082 scopus 로고    scopus 로고
    • Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture
    • 11071754 10.1006/dbio.2000.9912 1:CAS:528:DC%2BD3cXnvVeks70%3D
    • Amit M, Carpenter MK, Inokuma MS, et al. Clonally derived human embryonic stem cell lines maintain pluripotency and proliferative potential for prolonged periods of culture. Dev Biol. 2000;227:271-8.
    • (2000) Dev Biol , vol.227 , pp. 271-278
    • Amit, M.1    Carpenter, M.K.2    Inokuma, M.S.3
  • 2
    • 0038724202 scopus 로고    scopus 로고
    • Human feeder layers for human embryonic stem cells
    • 12606388 10.1095/biolreprod.102.012583 1:CAS:528:DC%2BD3sXks1Ggsb8%3D
    • Amit M, Margulets V, Segev H, et al. Human feeder layers for human embryonic stem cells. Biol Reprod. 2003;68:2150-6.
    • (2003) Biol Reprod , vol.68 , pp. 2150-2156
    • Amit, M.1    Margulets, V.2    Segev, H.3
  • 4
    • 33744758028 scopus 로고    scopus 로고
    • The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis
    • 16644866 10.1093/hmg/ddl112 1:CAS:528:DC%2BD28Xksl2hu7c%3D
    • Armstrong L, Hughes O, Yung S, et al. The role of PI3K/AKT, MAPK/ERK and NFkappabeta signalling in the maintenance of human embryonic stem cell pluripotency and viability highlighted by transcriptional profiling and functional analysis. Hum Mol Genet. 2006;15(11):1894-913.
    • (2006) Hum Mol Genet , vol.15 , Issue.11 , pp. 1894-1913
    • Armstrong, L.1    Hughes, O.2    Yung, S.3
  • 5
    • 16444373230 scopus 로고    scopus 로고
    • Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers
    • 15790770 10.1634/stemcells.2004-0279 1:CAS:528:DC%2BD2MXjslGjsrY%3D
    • Beattie GM, Lopez AD, Bucay N, et al. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells. 2005;23:489-95.
    • (2005) Stem Cells , vol.23 , pp. 489-495
    • Beattie, G.M.1    Lopez, A.D.2    Bucay, N.3
  • 6
    • 34548307176 scopus 로고    scopus 로고
    • IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro
    • 17625568 10.1038/nature06027 1:CAS:528:DC%2BD2sXps12ns7g%3D
    • Bendall SC, Stewart MH, Menendez P, et al. IGF and FGF cooperatively establish the regulatory stem cell niche of pluripotent human cells in vitro. Nature. 2007;448:1015-21.
    • (2007) Nature , vol.448 , pp. 1015-1021
    • Bendall, S.C.1    Stewart, M.H.2    Menendez, P.3
  • 7
    • 70350776634 scopus 로고    scopus 로고
    • Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology
    • 19327010 10.1089/scd.2008.0410
    • Brafman DA, Shah KD, Fellner T, Chien S, Willert K. Defining long-term maintenance conditions of human embryonic stem cells with arrayed cellular microenvironment technology. Stem Cells Dev. 2009;18(8):1141-54.
    • (2009) Stem Cells Dev , vol.18 , Issue.8 , pp. 1141-1154
    • Brafman, D.A.1    Shah, K.D.2    Fellner, T.3    Chien, S.4    Willert, K.5
  • 8
    • 77957298109 scopus 로고    scopus 로고
    • Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces
    • 20817292 10.1016/j.biomaterials.2010.08.007 1:CAS:528:DC%2BC3cXht1egsLnN
    • Brafman DA, Chang CW, Fernandez A, Willert K, Varghese S, Chien S. Long-term human pluripotent stem cell self-renewal on synthetic polymer surfaces. Biomaterials. 2010;31(34):9135-44.
    • (2010) Biomaterials , vol.31 , Issue.34 , pp. 9135-9144
    • Brafman, D.A.1    Chang, C.W.2    Fernandez, A.3    Willert, K.4    Varghese, S.5    Chien, S.6
  • 9
    • 19944426528 scopus 로고    scopus 로고
    • Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001
    • 15684826 10.1089/scd.2004.13.585 1:CAS:528:DC%2BD2MXnt1WksA%3D%3D
    • Brimble SN, Zeng X, Weiler DA, et al. Karyotypic stability, genotyping, differentiation, feeder-free maintenance, and gene expression sampling in three human embryonic stem cell lines derived prior to August 9, 2001. Stem Cells Dev. 2004;13(6):585-97.
    • (2004) Stem Cells Dev , vol.13 , Issue.6 , pp. 585-597
    • Brimble, S.N.1    Zeng, X.2    Weiler, D.A.3
  • 12
    • 65349131924 scopus 로고    scopus 로고
    • Engineered microenvironments for controlled stem cell differentiation
    • 18694293 10.1089/ten.tea.2008.0131 1:CAS:528:DC%2BD1MXhs1Cjs7w%3D
    • Burdick JA, Vunjak-Novakovic GV. Engineered microenvironments for controlled stem cell differentiation. Tissue Eng Part A. 2009;15:205-19.
    • (2009) Tissue Eng Part A , vol.15 , pp. 205-219
    • Burdick, J.A.1    Vunjak-Novakovic, G.V.2
  • 13
    • 77952094421 scopus 로고    scopus 로고
    • Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines
    • 10.1007/s11626-010-9291-5
    • Camarasa MV, Kerr RW, Sneddon SF, et al. Derivation of Man-1 and Man-2 research grade human embryonic stem cell lines. In Vitro Cell Dev Biol-Animal. 2010;46:386-94.
    • (2010) Vitro Cell Dev Biol-Animal , vol.46 , pp. 386-394
    • Camarasa, M.V.1    Kerr, R.W.2    Sneddon, S.F.3
  • 14
    • 46749138709 scopus 로고    scopus 로고
    • Characterization of six new human embryonic stem cell lines (HSF7, -8, -9, -10, -12, and -13) derived under minimal-animal component conditions
    • 18513167 10.1089/scd.2007.0216 1:CAS:528:DC%2BD1cXnslKkt70%3D
    • Chavez SL, Meneses JJ, Nguyen HN, Kim SK, Pera RA. Characterization of six new human embryonic stem cell lines (HSF7, -8, -9, -10, -12, and -13) derived under minimal-animal component conditions. Stem Cells Dev. 2008;17(3):535-46.
    • (2008) Stem Cells Dev , vol.17 , Issue.3 , pp. 535-546
    • Chavez, S.L.1    Meneses, J.J.2    Nguyen, H.N.3    Kim, S.K.4    Pera, R.A.5
  • 15
    • 67449141636 scopus 로고    scopus 로고
    • Novel autogenic feeders derived from human embryonic stem cells (hESCs) support an undifferentiated status of hESCs in xeno-free culture conditions
    • 19202140 10.1093/humrep/dep003 1:CAS:528:DC%2BD1MXksFejurw%3D
    • Chen HF, Chuang CY, Shieh YK, Chang HW, Ho HN, Kuo HC. Novel autogenic feeders derived from human embryonic stem cells (hESCs) support an undifferentiated status of hESCs in xeno-free culture conditions. Hum Reprod. 2009;24(5):1114-25.
    • (2009) Hum Reprod , vol.24 , Issue.5 , pp. 1114-1125
    • Chen, H.F.1    Chuang, C.Y.2    Shieh, Y.K.3    Chang, H.W.4    Ho, H.N.5    Kuo, H.C.6
  • 16
    • 79955611823 scopus 로고    scopus 로고
    • Chemically defined conditions for human iPSC derivation and culture
    • 10.1038/nmeth.1593 21478862 10.1038/nmeth.1593 1:CAS:528: DC%2BC3MXksFWnt7o%3D
    • Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, et al. Chemically defined conditions for human iPSC derivation and culture. Nat Methods. 2011;8(5):424-9. doi: 10.1038/nmeth.1593.
    • (2011) Nat Methods , vol.8 , Issue.5 , pp. 424-429
    • Chen, G.1    Gulbranson, D.R.2    Hou, Z.3    Bolin, J.M.4    Ruotti, V.5    Probasco, M.D.6
  • 17
    • 35848930381 scopus 로고    scopus 로고
    • The generation of six clinical-grade human embryonic stem cell lines
    • 10.1016/j.stem.2007.10.004 1:CAS:528:DC%2BD2sXhsVahtrvN
    • Crook JM, Peura TT, Kravets L, et al. The generation of six clinical-grade human embryonic stem cell lines. Cell Stem Cell. 2007;1(5):490-4.
    • (2007) Cell Stem Cell , vol.1 , Issue.5 , pp. 490-494
    • Crook, J.M.1    Peura, T.T.2    Kravets, L.3
  • 18
    • 33645751438 scopus 로고    scopus 로고
    • Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features
    • 16501172 10.1242/dev.02286 1:CAS:528:DC%2BD28Xjs1Srt78%3D
    • Darr H, Mayshar Y, Benvenisty N. Overexpression of NANOG in human ES cells enables feeder-free growth while inducing primitive ectoderm features. Development. 2006;133:1193-201.
    • (2006) Development , vol.133 , pp. 1193-1201
    • Darr, H.1    Mayshar, Y.2    Benvenisty, N.3
  • 19
    • 78649709231 scopus 로고    scopus 로고
    • Defined substrates for pluripotent stem cells: Are we there yet?
    • 21116246 10.1038/nmeth1210-967 1:CAS:528:DC%2BC3cXhsVOrsbbK
    • Elefanty AG, Stanley EG. Defined substrates for pluripotent stem cells: are we there yet? Nat Methods. 2010;7:967-8.
    • (2010) Nat Methods , vol.7 , pp. 967-968
    • Elefanty, A.G.1    Stanley, E.G.2
  • 20
    • 33749538618 scopus 로고    scopus 로고
    • Derivation of a xeno-free human embryonic stem cell line
    • 16741223 10.1634/stemcells.2006-0130
    • Ellerstrom C, Strehl R, Moya K, et al. Derivation of a xeno-free human embryonic stem cell line. Stem Cells. 2006;24:2170-6.
    • (2006) Stem Cells , vol.24 , pp. 2170-2176
    • Ellerstrom, C.1    Strehl, R.2    Moya, K.3
  • 21
    • 33751227152 scopus 로고    scopus 로고
    • Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines
    • 17196096 10.1089/clo.2006.8.319 1:CAS:528:DC%2BD2sXkvVei
    • Fletcher JM, Ferrier PM, Gardner JO, et al. Variations in humanized and defined culture conditions supporting derivation of new human embryonic stem cell lines. Cloning Stem Cells. 2006;8(4):319-34.
    • (2006) Cloning Stem Cells , vol.8 , Issue.4 , pp. 319-334
    • Fletcher, J.M.1    Ferrier, P.M.2    Gardner, J.O.3
  • 22
    • 20944444684 scopus 로고    scopus 로고
    • Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders
    • 15866593 10.1016/j.fertnstert.2005.01.086
    • Genbacev O, Krtolica A, Zdravkovic T, et al. Serum-free derivation of human embryonic stem cell lines on human placental fibroblast feeders. Fertil Steril. 2005;83:1517-29.
    • (2005) Fertil Steril , vol.83 , pp. 1517-1529
    • Genbacev, O.1    Krtolica, A.2    Zdravkovic, T.3
  • 23
    • 67650659087 scopus 로고    scopus 로고
    • Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines
    • Hakala H, Rajala K, Ojala M, et al. Comparison of biomaterials and extracellular matrices as a culture platform for multiple, independently derived human embryonic stem cell lines. Tissue Engineering Part A, 2009;1775-1785.
    • (2009) Tissue Engineering Part A , pp. 1775-1785
    • Hakala, H.1    Rajala, K.2    Ojala, M.3
  • 24
    • 2942591062 scopus 로고    scopus 로고
    • Derivation, characterization, and differentiation of human embryonic stem cells
    • 15153613 10.1634/stemcells.22-3-367
    • Heins N, Englund MC, Sjöblom C, et al. Derivation, characterization, and differentiation of human embryonic stem cells. Stem Cells. 2004;22(3):367-76.
    • (2004) Stem Cells , vol.22 , Issue.3 , pp. 367-376
    • Heins, N.1    Englund, M.C.2    Sjöblom, C.3
  • 25
    • 10744225452 scopus 로고    scopus 로고
    • A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells
    • 12832363 10.1093/humrep/deg290
    • Hovatta O, Mikkola M, Gertow K, et al. A culture system using human foreskin fibroblasts as feeder cells allows production of human embryonic stem cells. Hum Reprod. 2003;18:1404-9.
    • (2003) Hum Reprod , vol.18 , pp. 1404-1409
    • Hovatta, O.1    Mikkola, M.2    Gertow, K.3
  • 26
    • 70350218049 scopus 로고    scopus 로고
    • Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials
    • 19222349 10.1089/scd.2008.0416 1:CAS:528:DC%2BD1MXhsFKjs7%2FL
    • Ilic D, Giritharan G, Zdravkovic T, et al. Derivation of human embryonic stem cell lines from biopsied blastomeres on human feeders with minimal exposure to xenomaterials. Stem Cells Dev. 2009;18(9):1343-9.
    • (2009) Stem Cells Dev , vol.18 , Issue.9 , pp. 1343-1349
    • Ilic, D.1    Giritharan, G.2    Zdravkovic, T.3
  • 27
    • 83755164231 scopus 로고    scopus 로고
    • Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions
    • 22029654 10.3109/14653249.2011.623692 1:CAS:528:DC%2BC3MXhs1CitLzI
    • Ilic D, Stephenson E, Wood V, et al. Derivation and feeder-free propagation of human embryonic stem cells under xeno-free conditions. Cytotherapy. 2012;14(1):122-8.
    • (2012) Cytotherapy , vol.14 , Issue.1 , pp. 122-128
    • Ilic, D.1    Stephenson, E.2    Wood, V.3
  • 28
    • 65349125318 scopus 로고    scopus 로고
    • Derivation and characterization of two sibling human embryonic stem cell lines from discarded Grade III embryos
    • 18616385 10.1089/scd.2008.0131 1:CAS:528:DC%2BD1MXkvVGgsbs%3D
    • Inamdar MS, Venu P, Srinivas MS, Rao K, VijayRaghavan K. Derivation and characterization of two sibling human embryonic stem cell lines from discarded Grade III embryos. Stem Cells Dev. 2009;18(3):423-33.
    • (2009) Stem Cells Dev , vol.18 , Issue.3 , pp. 423-433
    • Inamdar, M.S.1    Venu, P.2    Srinivas, M.S.3    Rao, K.4    Vijayraghavan, K.5
  • 29
    • 16444368386 scopus 로고    scopus 로고
    • Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells
    • 15790775 10.1634/stemcells.2004-0201 1:CAS:528:DC%2BD2MXjslGjs70%3D
    • Inzunza J, Gertow K, Stromberg MA, et al. Derivation of human embryonic stem cell lines in serum replacement medium using postnatal human fibroblasts as feeder cells. Stem Cells. 2005;23:544-9.
    • (2005) Stem Cells , vol.23 , pp. 544-549
    • Inzunza, J.1    Gertow, K.2    Stromberg, M.A.3
  • 30
    • 79960842007 scopus 로고    scopus 로고
    • Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells
    • 21774983 10.1016/j.biomaterials.2011.05.058 1:CAS:528: DC%2BC3MXpsFWhsLw%3D
    • Irwin EF, Gupta R, Dashti DC, Healy KE. Engineered polymer-media interfaces for the long-term self-renewal of human embryonic stem cells. Biomaterials. 2011;32(29):6912-9.
    • (2011) Biomaterials , vol.32 , Issue.29 , pp. 6912-6919
    • Irwin, E.F.1    Gupta, R.2    Dashti, D.C.3    Healy, K.E.4
  • 31
    • 78649687686 scopus 로고    scopus 로고
    • A defined glycosaminoglycan-binding substratum for human pluripotent stem cells
    • 21076418 10.1038/nmeth.1532 1:CAS:528:DC%2BC3cXhsVWmurjO
    • Klim JR, Li L, Wrighton PJ, Piekarczyk MS, Kiessling LL. A defined glycosaminoglycan-binding substratum for human pluripotent stem cells. Nat Methods. 2010;7(12):989-94.
    • (2010) Nat Methods , vol.7 , Issue.12 , pp. 989-994
    • Klim, J.R.1    Li, L.2    Wrighton, P.J.3    Piekarczyk, M.S.4    Kiessling, L.L.5
  • 32
    • 18744401357 scopus 로고    scopus 로고
    • Human embryonic stem cells derived without feeder cells
    • 15885296 10.1016/S0140-6736(05)66473-2 1:CAS:528:DC%2BD2MXktVGku7w%3D
    • Klimanskaya I, Chung Y, Meisner L, Johnson J, West M, Lanza R. Human embryonic stem cells derived without feeder cells. Lancet. 2005;365:1636-41.
    • (2005) Lancet , vol.365 , pp. 1636-1641
    • Klimanskaya, I.1    Chung, Y.2    Meisner, L.3    Johnson, J.4    West, M.5    Lanza, R.6
  • 33
    • 33748580104 scopus 로고    scopus 로고
    • Human embryonic stem cell lines derived from single blastomeres
    • 16929302 10.1038/nature05142 1:CAS:528:DC%2BD28Xht1aku7%2FP
    • Klimanskaya I, Chung Y, Becker S, Lu S, Lanza R. Human embryonic stem cell lines derived from single blastomeres. Nature. 2006;444:481-5.
    • (2006) Nature , vol.444 , pp. 481-485
    • Klimanskaya, I.1    Chung, Y.2    Becker, S.3    Lu, S.4    Lanza, R.5
  • 34
    • 65349099944 scopus 로고    scopus 로고
    • Derivation and characterization of two genetically unique human embryonic stem cell lines on in-house-derived human feeders
    • 18699724 10.1089/scd.2008.0234 1:CAS:528:DC%2BD1MXkvVGgt7g%3D
    • Kumar N, Hinduja I, Nagvenkar P, et al. Derivation and characterization of two genetically unique human embryonic stem cell lines on in-house-derived human feeders. Stem Cells Dev. 2009;18(3):435-45.
    • (2009) Stem Cells Dev , vol.18 , Issue.3 , pp. 435-445
    • Kumar, N.1    Hinduja, I.2    Nagvenkar, P.3
  • 35
    • 78650971216 scopus 로고    scopus 로고
    • Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in cultura
    • 21211785 10.1016/j.stem.2010.12.003 1:CAS:528:DC%2BC3MXjvF2ntA%3D%3D
    • Laurent LC, Ulitsky I, Slavin I, et al. Dynamic changes in the copy number of pluripotency and cell proliferation genes in human ESCs and iPSCs during reprogramming and time in cultura. Cell Stem Cell. 2011;8:106-18.
    • (2011) Cell Stem Cell , vol.8 , pp. 106-118
    • Laurent, L.C.1    Ulitsky, I.2    Slavin, I.3
  • 36
    • 80053119708 scopus 로고    scopus 로고
    • The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells
    • 21911256 10.1016/j.biomaterials.2011.07.058 1:CAS:528:DC%2BC3MXht1WmtrvF
    • Lee S, Kim J, Park TJ, Shin Y, Lee SY, Han YM, et al. The effects of the physical properties of culture substrates on the growth and differentiation of human embryonic stem cells. Biomaterials. 2011;32(34):8816-29.
    • (2011) Biomaterials , vol.32 , Issue.34 , pp. 8816-8829
    • Lee, S.1    Kim, J.2    Park, T.J.3    Shin, Y.4    Lee, S.Y.5    Han, Y.M.6
  • 37
    • 34249774273 scopus 로고    scopus 로고
    • Transplantation of 3D scaffolds seeded with human embryonic stem cells: Biological features of surrogate tissue and teratoma-forming potential
    • 17511565 10.2217/17460751.2.3.289 1:CAS:528:DC%2BD2sXnvFaju7o%3D
    • Lees JG, Lim SA, Croll T, et al. Transplantation of 3D scaffolds seeded with human embryonic stem cells: biological features of surrogate tissue and teratoma-forming potential. Regen Med. 2007;2(3):289-300.
    • (2007) Regen Med , vol.2 , Issue.3 , pp. 289-300
    • Lees, J.G.1    Lim, S.A.2    Croll, T.3
  • 38
    • 34547908513 scopus 로고    scopus 로고
    • Xeno-free derivation and culture of human embryonic stem cells: Current status, problems and challenges
    • 17667917 10.1038/cr.2007.61 1:CAS:528:DC%2BD2sXovFeqtrw%3D
    • Lei T, Jacob S, Ajil-Zaraa I, et al. Xeno-free derivation and culture of human embryonic stem cells: current status, problems and challenges. Cell Res. 2007;17(8):682-8.
    • (2007) Cell Res , vol.17 , Issue.8 , pp. 682-688
    • Lei, T.1    Jacob, S.2    Ajil-Zaraa, I.3
  • 39
    • 0242331650 scopus 로고    scopus 로고
    • Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds
    • 14561891 10.1073/pnas.1735463100 1:CAS:528:DC%2BD3sXoslKntLs%3D
    • Levenberg S, Huang N, Lavik E, Rogers A, Itskovitz-Eldor J, Langer R. Differentiation of human embryonic stem cells on three-dimensional polymer scaffolds. Proc Natl Acad Sci USA. 2003;100:12741-6.
    • (2003) Proc Natl Acad Sci USA , vol.100 , pp. 12741-12746
    • Levenberg, S.1    Huang, N.2    Lavik, E.3    Rogers, A.4    Itskovitz-Eldor, J.5    Langer, R.6
  • 40
    • 33744746323 scopus 로고    scopus 로고
    • Basic fibroblast growth factor support of human embryonic stem cell self-renewal
    • 16282444 10.1634/stemcells.2005-0247 1:CAS:528:DC%2BD28XhtFKitbjL
    • Levenstein ME, Ludwig TE, Xu RH, et al. Basic fibroblast growth factor support of human embryonic stem cell self-renewal. Stem Cells. 2006;24(3):568-74.
    • (2006) Stem Cells , vol.24 , Issue.3 , pp. 568-574
    • Levenstein, M.E.1    Ludwig, T.E.2    Xu, R.H.3
  • 41
    • 78650599763 scopus 로고    scopus 로고
    • Dynamic electromechanical hydrogel matrices for stem cell culture
    • 10.1002/adfm.201001519 1:CAS:528:DC%2BC3cXhs1Sltr3E
    • Lim HL, Chuang JC, Tran T, Arya G, Varghese S. Dynamic electromechanical hydrogel matrices for stem cell culture. Adv Funct Mater. 2011;21:55-63.
    • (2011) Adv Funct Mater , vol.21 , pp. 55-63
    • Lim, H.L.1    Chuang, J.C.2    Tran, T.3    Arya, G.4    Varghese, S.5
  • 42
    • 37149036248 scopus 로고    scopus 로고
    • A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure
    • 18040289 10.1038/cr.2007.97 1:CAS:528:DC%2BD2sXhsVaisrjL
    • Lin G, OuYang Q, Zhou X, et al. A highly homozygous and parthenogenetic human embryonic stem cell line derived from a one-pronuclear oocyte following in vitro fertilization procedure. Cell Res. 2007;17(12):999-1007.
    • (2007) Cell Res , vol.17 , Issue.12 , pp. 999-1007
    • Lin, G.1    Ouyang, Q.2    Zhou, X.3
  • 43
    • 33645804391 scopus 로고    scopus 로고
    • Defined culture conditions of human embryonic stem cells
    • 16595624 10.1073/pnas.0601383103 1:CAS:528:DC%2BD28XktFaisLo%3D
    • Lu J, Hou R, Booth CJ, Yang S, Snyder M. Defined culture conditions of human embryonic stem cells. PNAS. 2006;103(15):5688-93.
    • (2006) PNAS , vol.103 , Issue.15 , pp. 5688-5693
    • Lu, J.1    Hou, R.2    Booth, C.J.3    Yang, S.4    Snyder, M.5
  • 44
    • 32344438401 scopus 로고    scopus 로고
    • Derivation of human embryonic stem cells in defined conditions
    • 16388305 10.1038/nbt1177 1:CAS:528:DC%2BD28XhtFGqsrg%3D
    • Ludwig TE, Levenstein ME, Jones JM, et al. Derivation of human embryonic stem cells in defined conditions. Nat Biotechnol. 2006;24(2):185-7.
    • (2006) Nat Biotechnol , vol.24 , Issue.2 , pp. 185-187
    • Ludwig, T.E.1    Levenstein, M.E.2    Jones, J.M.3
  • 45
    • 37149049044 scopus 로고    scopus 로고
    • Derivation of human embryonic stem cell lines from parthenogenetic blastocysts
    • 18071366 10.1038/cr.2007.102 1:CAS:528:DC%2BD2sXhsVaisrnO
    • Mai Q, Yu Y, Li T, et al. Derivation of human embryonic stem cell lines from parthenogenetic blastocysts. Cell Res. 2007;17:1008-19.
    • (2007) Cell Res , vol.17 , pp. 1008-1019
    • Mai, Q.1    Yu, Y.2    Li, T.3
  • 46
    • 46749119848 scopus 로고    scopus 로고
    • A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture
    • 18513158 10.1089/scd.2007.0236 1:CAS:528:DC%2BD1cXnslKkt74%3D
    • Meng G, Liu S, Krawetz R, Chan M, Chernos J, Rancourt DE. A novel method for generating xeno-free human feeder cells for human embryonic stem cell culture. Stem Cells Dev. 2008;17:413-22.
    • (2008) Stem Cells Dev , vol.17 , pp. 413-422
    • Meng, G.1    Liu, S.2    Krawetz, R.3    Chan, M.4    Chernos, J.5    Rancourt, D.E.6
  • 47
    • 77950606859 scopus 로고    scopus 로고
    • Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture
    • 19883201 10.1089/scd.2009.0303 1:CAS:528:DC%2BC3cXktlygsb4%3D
    • Meng G, Liu S, Li X, Krawetz R, Rancourt DE. Extracellular matrix isolated from foreskin fibroblasts supports long-term xeno-free human embryonic stem cell culture. Stem Cells Dev. 2010;19(4):547-56.
    • (2010) Stem Cells Dev , vol.19 , Issue.4 , pp. 547-556
    • Meng, G.1    Liu, S.2    Li, X.3    Krawetz, R.4    Rancourt, D.E.5
  • 48
    • 67649959002 scopus 로고    scopus 로고
    • Feeder-free maintenance of hESCs in mesenchymal stem cell-conditioned media: Distinct requirements for TGF-β and IGF-II
    • 19308090 10.1038/cr.2009.35 1:CAS:528:DC%2BD1MXms1Omtb4%3D
    • Montes R, Ligero G, Sanchez L, et al. Feeder-free maintenance of hESCs in mesenchymal stem cell-conditioned media: distinct requirements for TGF-β and IGF-II. Cell Res. 2009;19:698-709.
    • (2009) Cell Res , vol.19 , pp. 698-709
    • Montes, R.1    Ligero, G.2    Sanchez, L.3
  • 49
    • 77952858910 scopus 로고    scopus 로고
    • Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum
    • 20525219 10.1186/1471-213X-10-60
    • Nagaoka M, Si-Tayeb K, Akaike T, Duncan SA. Culture of human pluripotent stem cells using completely defined conditions on a recombinant E-cadherin substratum. BMC Dev Biol. 2010;10:60.
    • (2010) BMC Dev Biol , vol.10 , pp. 60
    • Nagaoka, M.1    Si-Tayeb, K.2    Akaike, T.3    Duncan, S.A.4
  • 50
    • 46749118841 scopus 로고    scopus 로고
    • Use of xenofree matrices and molecularly-defined media to control human embryonic stem cell pluripotency: Effect of low physiological TGF-beta concentrations
    • 18513159 10.1089/scd.2007.0279 1:CAS:528:DC%2BD1cXnslKktrY%3D
    • Peiffer I, Barbet R, Zhou YP, et al. Use of xenofree matrices and molecularly-defined media to control human embryonic stem cell pluripotency: effect of low physiological TGF-beta concentrations. Stem Cells Dev. 2008;17(3):519-33.
    • (2008) Stem Cells Dev , vol.17 , Issue.3 , pp. 519-533
    • Peiffer, I.1    Barbet, R.2    Zhou, Y.P.3
  • 51
    • 64249114789 scopus 로고    scopus 로고
    • Stem cell bioprocessing: Fundamentals and principles
    • 19033137 10.1098/rsif.2008.0442 1:CAS:528:DC%2BD1MXktFKhsbk%3D
    • Placzek MR, Chung I-M, Macedo HM, et al. Stem cell bioprocessing: fundamentals and principles. J R Soc Interface. 2009;6:209-32.
    • (2009) J R Soc Interface , vol.6 , pp. 209-232
    • Placzek, M.R.1    Chung, I.-M.2    MacEdo, H.M.3
  • 52
    • 77952096519 scopus 로고    scopus 로고
    • Derivation and characterisation of the human embryonic stem cell lines, NOTT1 and NOTT2
    • 10.1007/s11626-010-9290-6
    • Priddle H, Allegrucci C, Burridge P, et al. Derivation and characterisation of the human embryonic stem cell lines, NOTT1 and NOTT2. In Vitro Cell Dev Biol - Animal. 2010;46:367-75.
    • (2010) Vitro Cell Dev Biol - Animal , vol.46 , pp. 367-375
    • Priddle, H.1    Allegrucci, C.2    Burridge, P.3
  • 53
    • 34447103025 scopus 로고    scopus 로고
    • Testing of nine different xeno-free culture media for human embryonic stem cell cultures
    • 17251355 10.1093/humrep/del523 1:CAS:528:DC%2BD2sXntlWmsr0%3D
    • Rajala K, Hakala H, Panula S, et al. Testing of nine different xeno-free culture media for human embryonic stem cell cultures. Hum Reprod. 2007;22(5):1231-8.
    • (2007) Hum Reprod , vol.22 , Issue.5 , pp. 1231-1238
    • Rajala, K.1    Hakala, H.2    Panula, S.3
  • 54
    • 77956337899 scopus 로고    scopus 로고
    • A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cells
    • 20419109 10.1371/journal.pone.0010246
    • Rajala K, Lindroos B, Hussein SM, et al. A defined and xeno-free culture method enabling the establishment of clinical-grade human embryonic, induced pluripotent and adipose stem cells. PLoS One. 2010;5(4):e10246.
    • (2010) PLoS One , vol.5 , Issue.4 , pp. 10246
    • Rajala, K.1    Lindroos, B.2    Hussein, S.M.3
  • 55
    • 68549115634 scopus 로고    scopus 로고
    • Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts
    • 19028986 10.1101/sqb.2008.73.038 1:CAS:528:DC%2BD1MXos1ert74%3D
    • Raya A, Rodríguez-Pizà I, Arán B, Consiglio A, Barri PN, Veiga A, et al. Generation of cardiomyocytes from new human embryonic stem cell lines derived from poor-quality blastocysts. Cold Spring Harb Symp Quant Biol. 2008;73:127-35.
    • (2008) Cold Spring Harb Symp Quant Biol , vol.73 , pp. 127-135
    • Raya, A.1    Rodríguez-Pizà, I.2    Arán, B.3    Consiglio, A.4    Barri, P.N.5    Veiga, A.6
  • 56
    • 0345688742 scopus 로고    scopus 로고
    • Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells
    • 12968109 10.1634/stemcells.21-5-546 1:CAS:528:DC%2BD3sXnvFeisrY%3D
    • Richards M, Tan S, Fong CY, Biswas A, Chan WK, Bongso A. Comparative evaluation of various human feeders for prolonged undifferentiated growth of human embryonic stem cells. Stem Cells. 2003;21:546-56.
    • (2003) Stem Cells , vol.21 , pp. 546-556
    • Richards, M.1    Tan, S.2    Fong, C.Y.3    Biswas, A.4    Chan, W.K.5    Bongso, A.6
  • 58
    • 84871263532 scopus 로고    scopus 로고
    • The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions
    • 23054358 10.1007/s10815-012-9866-z 1:STN:280:DC%2BC3s%2FktFCguw%3D%3D
    • Ruggeri RR, Watanabe Y, Meirelles F, Bressan FF, Frantz N, Bos-Mikich A. The use of parthenotegenetic and IVF bovine blastocysts as a model for the creation of human embryonic stem cells under defined conditions. J Assist Reprod Genet. 2012;29(10):1039-43.
    • (2012) J Assist Reprod Genet , vol.29 , Issue.10 , pp. 1039-1043
    • Ruggeri, R.R.1    Watanabe, Y.2    Meirelles, F.3    Bressan, F.F.4    Frantz, N.5    Bos-Mikich, A.6
  • 59
    • 33751011613 scopus 로고    scopus 로고
    • Transgenic human fetal fibroblasts as feeder layer for human embryonic stem cell lineage selection
    • 17105409 10.1089/scd.2006.15.741 1:CAS:528:DC%2BD28XhtF2ns7bO
    • Sidhu KS, Lie KH, Tuch BE. Transgenic human fetal fibroblasts as feeder layer for human embryonic stem cell lineage selection. Stem Cells Dev. 2006;15:741-7.
    • (2006) Stem Cells Dev , vol.15 , pp. 741-747
    • Sidhu, K.S.1    Lie, K.H.2    Tuch, B.E.3
  • 60
    • 33751217707 scopus 로고    scopus 로고
    • Culture conditions for human embryonic stem cells
    • 17071770 10.1530/rep.1.01079 1:CAS:528:DC%2BD28XhtlWktbrO
    • Skottman H, Hovatta O. Culture conditions for human embryonic stem cells. Reproduction. 2006;132:691-8.
    • (2006) Reproduction , vol.132 , pp. 691-698
    • Skottman, H.1    Hovatta, O.2
  • 61
    • 33646541710 scopus 로고    scopus 로고
    • The derivation of clinical-grade human embryonic stem cell lines
    • 16716780 10.1016/j.febslet.2006.03.083 1:CAS:528:DC%2BD28XltVKnt7s%3D
    • Skottman H, Dilber MS, Hovatta O. The derivation of clinical-grade human embryonic stem cell lines. FEBS Lett. 2006;580:2875-8.
    • (2006) FEBS Lett , vol.580 , pp. 2875-2878
    • Skottman, H.1    Dilber, M.S.2    Hovatta, O.3
  • 62
    • 57449090630 scopus 로고    scopus 로고
    • Recurrent chromosomal abnormalities in human embryonic stem cells
    • 10.1038/nbt.1510 1:CAS:528:DC%2BD1cXhsVWjtL3M
    • Spits C, Mateizel I, Geens M, et al. Recurrent chromosomal abnormalities in human embryonic stem cells. Nat Biotechnology. 2008;26:1361-3.
    • (2008) Nat Biotechnology , vol.26 , pp. 1361-1363
    • Spits, C.1    Mateizel, I.2    Geens, M.3
  • 63
    • 77950677122 scopus 로고    scopus 로고
    • Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension
    • 10.1038/nbt.1616 1:CAS:528:DC%2BC3cXjvFartr0%3D
    • Steiner D, Khaner H, Cohen M, et al. Derivation, propagation and controlled differentiation of human embryonic stem cells in suspension. Nat Biotechnology. 2010;28(4):361-6.
    • (2010) Nat Biotechnology , vol.28 , Issue.4 , pp. 361-366
    • Steiner, D.1    Khaner, H.2    Cohen, M.3
  • 64
    • 15544375987 scopus 로고    scopus 로고
    • An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells
    • 15749925 10.1634/stemcells.2004-0137 1:CAS:528:DC%2BD2MXjtFyjtLc%3D
    • Stojkovic P, Lako M, Stewart R, et al. An autogeneic feeder cell system that efficiently supports growth of undifferentiated human embryonic stem cells. Stem Cells. 2005;23(3):306-14.
    • (2005) Stem Cells , vol.23 , Issue.3 , pp. 306-314
    • Stojkovic, P.1    Lako, M.2    Stewart, R.3
  • 65
    • 23844556152 scopus 로고    scopus 로고
    • Human-serum matrix supports undifferentiated growth of human embryonic stem cells
    • 15888688 10.1634/stemcells.2004-0326 1:CAS:528:DC%2BD2MXhtVemsrbN
    • Stojkovic P, Lako M, Przyborski S, et al. Human-serum matrix supports undifferentiated growth of human embryonic stem cells. Stem Cells. 2005;23(7):895-902.
    • (2005) Stem Cells , vol.23 , Issue.7 , pp. 895-902
    • Stojkovic, P.1    Lako, M.2    Przyborski, S.3
  • 67
    • 40449092159 scopus 로고    scopus 로고
    • Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines
    • 17959612 10.1093/humrep/dem335
    • Ström S, Inzunza J, Grinnemo K, et al. Mechanical isolation of the inner cell mass is effective in derivation of new human embryonic stem cell lines. Hum Reprod. 2007;22(12):3051-8.
    • (2007) Hum Reprod , vol.22 , Issue.12 , pp. 3051-3058
    • Ström, S.1    Inzunza, J.2    Grinnemo, K.3
  • 68
    • 84867800506 scopus 로고    scopus 로고
    • FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast
    • 22724507 10.1089/scd.2012.0099 1:CAS:528:DC%2BC38XhsFCgsLfN
    • Sudheer S, Bhushan R, Fauler B, Lehrach H, Adjaye J. FGF inhibition directs BMP4-mediated differentiation of human embryonic stem cells to syncytiotrophoblast. Stem Cells Dev. 2012;21(16):2987-3000.
    • (2012) Stem Cells Dev , vol.21 , Issue.16 , pp. 2987-3000
    • Sudheer, S.1    Bhushan, R.2    Fauler, B.3    Lehrach, H.4    Adjaye, J.5
  • 69
    • 0032491416 scopus 로고    scopus 로고
    • Embryonic stem cell lines derived from human blastocysts
    • 9804556 10.1126/science.282.5391.1145 1:CAS:528:DyaK1cXntleisLg%3D
    • Thomson JA, Itskovitz-Eldor J, Shapiro SS, et al. Embryonic stem cell lines derived from human blastocysts. Science. 1998;282(5391):1145-7.
    • (1998) Science , vol.282 , Issue.5391 , pp. 1145-1147
    • Thomson, J.A.1    Itskovitz-Eldor, J.2    Shapiro, S.S.3
  • 70
    • 77952098043 scopus 로고    scopus 로고
    • High-efficiency derivation of human embryonic stem cell lines following pre-implantation genetic diagnosis in Vitro
    • 10.1007/s11626-010-9300-8
    • Tropel P, Tournois J, Côme J, et al. High-efficiency derivation of human embryonic stem cell lines following pre-implantation genetic diagnosis In Vitro. Cell Dev Biol-Animal. 2010;46:376-85.
    • (2010) Cell Dev Biol-Animal , vol.46 , pp. 376-385
    • Tropel, P.1    Tournois, J.2    Côme, J.3
  • 71
    • 79952165906 scopus 로고    scopus 로고
    • Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells
    • 21364903 1:CAS:528:DC%2BC3MXjtVyktLw%3D
    • Vaajasaari H, Ilmarinen T, Juuti-Uusitalo K, et al. Toward the defined and xeno-free differentiation of functional human pluripotent stem cell-derived retinal pigment epithelial cells. Mol Vis. 2011;17:558-75.
    • (2011) Mol Vis , vol.17 , pp. 558-575
    • Vaajasaari, H.1    Ilmarinen, T.2    Juuti-Uusitalo, K.3
  • 72
    • 67650567329 scopus 로고    scopus 로고
    • Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways
    • 10.1371/journal.pone.0006082 19564924 10.1371/journal.pone.0006082
    • Vallier L, Touboul T, Chng Z, Brimpari M, Hannan N, et al. Early cell fate decisions of human embryonic stem cells and mouse epiblast stem cells are controlled by the same signalling pathways. PLoS One. 2009;4(6):e6082. doi: 10.1371/journal.pone.0006082.
    • (2009) PLoS One , vol.4 , Issue.6 , pp. 6082
    • Vallier, L.1    Touboul, T.2    Chng, Z.3    Brimpari, M.4    Hannan, N.5
  • 73
    • 84872457949 scopus 로고    scopus 로고
    • The combination of inhibitors of FGF/MEK/Erk and GSK3β signaling increases the Number of OCT3/4- and NANOG-positive cells in the human inner cell mass, but does not improve stem cell derivation
    • ahead of print. doi: 10.1089/scd.2012.0256
    • Van der Jeught M, O'Leary T, Ghimire S, Lierman S, Duggal G, Versieren K, et al. The combination of inhibitors of FGF/MEK/Erk and GSK3β signaling increases the Number of OCT3/4- and NANOG-positive cells in the human inner cell mass, but does not improve stem cell derivation. Stem Cells Dev. 2012. ahead of print. doi: 10.1089/scd.2012.0256.
    • (2012) Stem Cells Dev.
    • Van Der Jeught, M.1    O'Leary, T.2    Ghimire, S.3    Lierman, S.4    Duggal, G.5    Versieren, K.6
  • 74
    • 77953254106 scopus 로고    scopus 로고
    • Synthetic polymer coatings for long-term growth of human embryonic stem cells
    • 10.1038/nbt.1631 1:CAS:528:DC%2BC3cXmslWmtb0%3D
    • Villa-Diaz LG, Nandivada H, Ding J, et al. Synthetic polymer coatings for long-term growth of human embryonic stem cells. Nat Biotechnology. 2010;28(6):581-3.
    • (2010) Nat Biotechnology , vol.28 , Issue.6 , pp. 581-583
    • Villa-Diaz, L.G.1    Nandivada, H.2    Ding, J.3
  • 75
    • 84876286146 scopus 로고    scopus 로고
    • Scaffold materials for hES cell culture and differentiation
    • Ducheyne P, Healy KE, Hutmacher DW, Grainger DW, Kirkpatrick CJ (eds.). Elsevier
    • Willerth S, Schaffer D. Scaffold materials for hES cell culture and differentiation. In: Ducheyne P, Healy KE, Hutmacher DW, Grainger DW, Kirkpatrick CJ (eds.) Comprehensive Biomaterials (Vol. 5) Elsevier. 2011. p. 95-113.
    • (2011) Comprehensive Biomaterials , vol.5 , pp. 95-113
    • Willerth, S.1    Schaffer, D.2
  • 76
    • 18744375483 scopus 로고    scopus 로고
    • Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells
    • 15782187 10.1038/nmeth744 1:CAS:528:DC%2BD2MXisVGisrc%3D
    • Xu RH, Peck RM, Li DS, Feng X, Ludwig T, Thomson JA. Basic FGF and suppression of BMP signaling sustain undifferentiated proliferation of human ES cells. Nat Methods. 2005;2(3):185-90.
    • (2005) Nat Methods , vol.2 , Issue.3 , pp. 185-190
    • Xu, R.H.1    Peck, R.M.2    Li, D.S.3    Feng, X.4    Ludwig, T.5    Thomson, J.A.6
  • 77
    • 33646474117 scopus 로고    scopus 로고
    • Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions
    • 16632596 10.1073/pnas.0602280103 1:CAS:528:DC%2BD28XkslCksLg%3D
    • Yao S, Chen S, Clark J, et al. Long-term self-renewal and directed differentiation of human embryonic stem cells in chemically defined conditions. PNAS. 2006;103(18):6907-12.
    • (2006) PNAS , vol.103 , Issue.18 , pp. 6907-6912
    • Yao, S.1    Chen, S.2    Clark, J.3
  • 78
    • 79551514298 scopus 로고    scopus 로고
    • Defining a threshold surface density of vitronectin for the stable expansion of human embryonic stem cells
    • 20726687 10.1089/ten.tec.2010.0328 1:CAS:528:DC%2BC3MXht1yrt74%3D
    • Yap LY, Li J, Phang IY, Ong LT, Ow JZ, Goh JC, et al. Defining a threshold surface density of vitronectin for the stable expansion of human embryonic stem cells. Tissue Eng Part C Methods. 2011;17(2):193-207.
    • (2011) Tissue Eng Part C Methods , vol.17 , Issue.2 , pp. 193-207
    • Yap, L.Y.1    Li, J.2    Phang, I.Y.3    Ong, L.T.4    Ow, J.Z.5    Goh, J.C.6
  • 79
    • 33846044682 scopus 로고    scopus 로고
    • Derivation of human embryonic stem cells from developing and arrested embryos
    • 16990582 10.1634/stemcells.2006-0377 1:CAS:528:DC%2BD2sXkslCltQ%3D%3D
    • Zhang X, Stojkovic P, Przyborski S, et al. Derivation of human embryonic stem cells from developing and arrested embryos. Stem Cells. 2006;24:2669-76.
    • (2006) Stem Cells , vol.24 , pp. 2669-2676
    • Zhang, X.1    Stojkovic, P.2    Przyborski, S.3
  • 80
    • 51849140558 scopus 로고    scopus 로고
    • Human feeder cells support establishment and definitive endoderm differentiation of human embryonic stem cells
    • 18788933 10.1089/scd.2007.0186 1:CAS:528:DC%2BD1cXhtFaisrnJ
    • Zhou J, Ou-Yang Q, Li J, Zhou X-Y, Lin G, Lu G-X. Human feeder cells support establishment and definitive endoderm differentiation of human embryonic stem cells. Stem Cells Dev. 2008;17:737-50.
    • (2008) Stem Cells Dev , vol.17 , pp. 737-750
    • Zhou, J.1    Ou-Yang, Q.2    Li, J.3    Zhou, X.-Y.4    Lin, G.5    Lu, G.-X.6


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.