A screen for over-secretion of proteins by yeast based on a dual component cellular phosphatase and immuno-chromogenic stain for exported bacterial alkaline phosphatase reporter

Microbial Cell Factories

Volumn 12, Issue 1, 2013, Pages

  Nallaseth, Ferez S ^a   Anderson, Stephen ^a  

^a RUTGERS UNIVERSITY   (United States)

Author keywords

sec EAP ( secMAT; AOX1; Cellular phosphatase reference activity; Chemical mutagenesis; Dual component chromogenic and immuno chromogenic stains; E.coli Alkaline Phosphatase); Over secretor mutants; P. pastoris; Protein export

Indexed keywords

ALKALINE PHOSPHATASE; ESCHERICHIA COLI ALKALINE PHOSPHATASE; LEVULINIC ACID; UNCLASSIFIED DRUG;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; MUTAGENESIS; NONHUMAN; PICHIA PASTORIS; PROTEIN ANALYSIS; PROTEIN SECRETION; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION;

ALDEHYDE OXIDASE; ALKALINE PHOSPHATASE; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENETIC LOCI; GENOTYPE; MUTAGENESIS; PHENOTYPE; PICHIA; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS);

EID: 84876286909     PISSN: None     EISSN: 14752859     Source Type: Journal    
DOI: 10.1186/1475-2859-12-36     Document Type: Article

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