Functional expression, purification, and characterization of the extra stable human placental alkaline phosphatase in the Pichia pastoris system

Protein Expression and Purification

Volumn 36, Issue 1, 2004, Pages 90-99

  Chen, Yu Hou ^a   Chang, Tsu Chung ^a   Chang, Gu Gang ^b  

^a NATIONAL DEFENSE MEDICAL CENTER   (Taiwan)

^b NATIONAL YANG MING UNIVERSITY   (Taiwan)

Author keywords

Analytical ultracentrifugation; Enzyme cloning; Enzyme stability; Fluorescence; Gel permeation chromatography; Protein expression; Protein folding

Indexed keywords

ALCOHOL DEHYDROGENASE; ALCOHOL OXIDASE; CARCINOPLACENTAL ISOENZYMES; ISOENZYME; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; ENZYME STABILITY; GENE VECTOR; GENETICS; HUMAN; ISOLATION AND PURIFICATION; MOLECULAR CLONING; PICHIA; PROMOTER REGION; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE;

ALCOHOL OXIDOREDUCTASES; CLONING, MOLECULAR; ENZYME STABILITY; GENETIC VECTORS; HUMANS; ISOENZYMES; PICHIA; PROMOTER REGIONS (GENETICS); PROTEIN FOLDING; PROTEIN STRUCTURE, TERTIARY; RECOMBINANT PROTEINS;

PICHIA; PICHIA PASTORIS;

EID: 3042598420     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.03.006     Document Type: Article

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