Isolation of microarray-quality RNA from primary human cells after intracellular immunostaining and fluorescence-activated cell sorting

Journal of Immunological Methods

Volumn 391, Issue 1-2, 2013, Pages 22-30

  Iglesias Ussel, Maria ^a   Marchionni, Luigi ^b   Romerio, Fabio ^a  

^a UNIVERSITY OF MARYLAND SCHOOL OF MEDICINE   (United States)

^b JOHNS HOPKINS UNIVERSITY   (United States)

Author keywords

FACS; Fixation; Intracellular staining; Microarray; Permeabilization; RNA

Indexed keywords

ARTICLE; CONTROLLED STUDY; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION PROFILING; GENETIC ANALYSIS; GENETIC ASSOCIATION; HUMAN; HUMAN CELL; IMMUNOHISTOCHEMISTRY; MICROARRAY ANALYSIS; PRIORITY JOURNAL; RNA DEGRADATION; RNA ISOLATION;

CD4-POSITIVE T-LYMPHOCYTES; CELL SEPARATION; CELLS, CULTURED; FLOW CYTOMETRY; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; GAG GENE PRODUCTS, HUMAN IMMUNODEFICIENCY VIRUS; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HIV-1; HUMANS; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PRIMARY CELL CULTURE; REPRODUCIBILITY OF RESULTS; RNA; RNA STABILITY;

EID: 84876134930     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.02.003     Document Type: Article

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