Factors in tissue handling and processing that impact RNA obtained from formalin-fixed, paraffin-embedded tissue

Journal of Histochemistry and Cytochemistry

Volumn 56, Issue 11, 2008, Pages 1033-1042

  Chung, Joon Yong ^a   Braunschweig, Till ^a   Williams, Reginald ^a   Guerrero, Natalie ^a   Hoffmann, Karl M ^c   Kwon, Mijung ^b   Song, Young K ^b   Libutti, Steven K ^b   Hewitt, Stephen M ^a,d  

^a NATIONAL INSTITUTES OF HEALTH   (United States)

^b NATIONAL CANCER INSTITUTE   (United States)

^c NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES   (United States)

^d Advanced Technology Center   (United States)

Author keywords

Formalin fixed; Gene expression; Paraffin embedded; Real time RT PCR; RNA integrity; Tissue fixation and processing

Indexed keywords

BUFFER; FORMALDEHYDE; PARAFFIN; PHOSPHATE; RNA;

ANALYTIC METHOD; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CONTROLLED STUDY; FEMALE; GENE; GENE EXPRESSION; INFORMATION; MOUSE; NONHUMAN; PATHOLOGY; PRIORITY JOURNAL; QUANTITATIVE ANALYSIS; RAT; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SURGICAL TECHNIQUE; TISSUE FIXATION;

ANIMALS; BUFFERS; ELECTROPHORESIS, CAPILLARY; FEMALE; FIXATIVES; FORMALDEHYDE; KIDNEY; PARAFFIN EMBEDDING; RATS; RATS, SPRAGUE-DAWLEY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA; RNA, MESSENGER; SPECIMEN HANDLING;

EID: 54349086286     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1369/jhc.2008.951863     Document Type: Article

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