Protective effect of lentivirus-mediated sirna targeting adamts-5 on cartilage degradation in a rat model of osteoarthritis

International Journal of Molecular Medicine

Volumn 31, Issue 5, 2013, Pages 1222-1228

  Chu, Xiangyu ^a   You, Hongbo ^a   Yuan, Xuefeng ^a   Zhao, Wenbin ^a   Li, Wenkai ^a   Guo, Xin ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

A disintegrin and metalloproteinase with thrombopondin motifs 5; Cartilage; Osteoarthritis; RNA interference

Indexed keywords

AGGRECANASE 2; PLASMID DNA; SMALL INTERFERING RNA;

ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; ARTICULAR CARTILAGE; CARTILAGE CELL; CARTILAGE DEGENERATION; CONTROLLED STUDY; DISEASE COURSE; DOWN REGULATION; GENE EXPRESSION; GENE REPRESSION; GENE SILENCING; GENE TARGETING; IMMUNOHISTOCHEMISTRY; IN VIVO STUDY; KNEE CRUCIATE LIGAMENT; LENTIVIRINAE; MALE; MENISCECTOMY; MONOLAYER CULTURE; NONHUMAN; OSTEOARTHRITIS; PRIORITY JOURNAL; RAT; REAL TIME POLYMERASE CHAIN REACTION; RNA ISOLATION; SPRAGUE DAWLEY RAT; TRANSIENT TRANSFECTION; VECTOR CONTROL; WESTERN BLOTTING;

ADAM PROTEINS; ANIMALS; BASE SEQUENCE; BLOTTING, WESTERN; CARTILAGE, ARTICULAR; CELL SHAPE; CHONDROCYTES; COLLAGEN TYPE II; DISEASE MODELS, ANIMAL; GENE EXPRESSION REGULATION; HUMANS; JOINTS; LENTIVIRUS; MALE; MOLECULAR SEQUENCE DATA; OSTEOARTHRITIS; RATS; RATS, SPRAGUE-DAWLEY; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84876116755     PISSN: 11073756     EISSN: 1791244X     Source Type: Journal    
DOI: 10.3892/ijmm.2013.1318     Document Type: Article

References (23)

1. Sarzi-Puttini P, Cimmino MA, Scarpa R, et al: Osteoarthritis: An overview of the disease and its treatment strategies. Semin Arthritis Rheum 35 (1 Suppl 1): 1-10, 2005.
2. Hayami T, Pickarski M, Wesolowski GA, et al: The role of subchondral bone remodeling in osteoarthritis: Reduction of cartilage degeneration and prevention of osteophyte formation by alendronate in the rat anterior cruciate ligament transection model. Arthritis Rheum 50: 1193-1206, 2004.
3. Stanton H, Rogerson FM, East CJ, et al: ADAMTS5 is the major aggrecanase in mouse cartilage in vivo and in vitro. Nature 434: 648-652, 2005.
4. Glasson SS, Askew R, Sheppard B, et al: Deletion of active ADAMTS5 prevents cartilage degradation in a murine model of osteoarthritis. Nature 434: 644-648, 2005.
5. Fire A, Xu S, Montgomery MK, Kostas SA, Driver SE and Mello CC: Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 391: 806-811, 1998.
6. Martin SE and Caplen NJ: Applications of RNA interference in mammalian systems. Annu Rev Genomics Hum Genet 8: 81-108, 2007.
7. Campbell TN and Choy FY: RNA interference: Past, present and future. Curr Issues Mol Biol 7: 1-6, 2005.
8. Sen GL and Blau HM: A brief history of RNAi: The silence of the genes. FASEB J 20: 1293-1299, 2006.
9. Naldini L, Blomer U, Gallay P, et al: In vivo gene delivery and stable transduction of nondividing cells by a lentiviral vector. Science 272: 263-267, 1996.
10. Zufferey R, Dull T, Mandel RJ, et al: Self-inactivating lentivirus vector for safe and efficient in vivo gene delivery. J Virol 72: 9873-9880, 1998.
11. Hu DN, Yang PY, Ku MC, Chu CH, Lim AY and Hwang MH: Isolation and cultivation of human articular chondrocytes. Kaohsiung J Med Sci 18: 113-120, 2002.
12. Durigova M, Troeberg L, Nagase H, Roughley PJ and Mort JS: Involvement of ADAMTS5 and hyaluronidase in aggrecan degradation and release from OSM-stimulated cartilage. Eur Cell Mater 21: 31-45, 2011.
13. Seki S, Asanuma-Abe Y, Masuda K, et al: Effect of small interference RNA (siRNA) for ADAMTS5 on intervertebral disc degeneration in the rabbit anular needle-puncture model. Arthritis Res Ther 11: R166, 2009.
14. Hummon AB, Lim SR, Difilippantonio MJ and Ried T: Isolation and solubilization of proteins after TRIzol extraction of RNA and DNA from patient material following prolonged storage. Biotechniques 42: 467-470, 472, 2007.
15. Gouze E, Pawliuk R, Pilapil C, et al: In vivo gene delivery to synovium by lentiviral vectors. Mol Ther 5: 397-404, 2002.
16. Appleton CT, McErlain DD, Pitelka V, et al: Forced mobilization accelerates pathogenesis: Characterization of a preclinical surgical model of osteoarthritis. Arthritis Res Ther 9: R13, 2007.
17. Rozas G, Guerra MJ and Labandeira-Garcia JL: An automated rotarod method for quantitative drug-free evaluation of overall motor deficits in rat models of parkinsonism. Brain Res Brain Res Protoc 2: 75-84, 1997.
18. Martins MA, de Castro Bastos L and Tonussi CR: Formalin injection into knee joints of rats: Pharmacologic characterization of a deep somatic nociceptive model. J Pain 7: 100-107, 2006.
19. Custers RJ, Creemers LB, Verbout AJ, van Rijen MH, Dhert WJ and Saris DB: Reliability, reproducibility and variability of the traditional Histologic/Histochemical Grading System vs the new OARSI Osteoarthritis Cartilage Histopathology Assessment System. Osteoarthritis Cartilage 15: 1241-1248, 2007.
20. Broom ND: Further insights into the structural principles governing the function of articular cartilage. J Anat 139: 275-294, 1984.
21. Jones MD, Tran CW, Li G, Maksymowych WP, Zernicke RF and Doschak MR: In vivo microfocal computed tomography and micro-magnetic resonance imaging evaluation of antiresorptive and antiinflammatory drugs as preventive treatments of osteoarthritis in the rat. Arthritis Rheum 62: 2726-2735, 2010.
22. Song RH, Tortorella MD, Malfait AM, et al: Aggrecan degradation in human articular cartilage explants is mediated by both ADAMTS-4 and ADAMTS-5. Arthritis Rheum 56: 575-585, 2007.
23. Majumdar MK, Askew R, Schelling S, et al: Double-knockout of ADAMTS-4 and ADAMTS-5 in mice results in physiologically normal animals and prevents the progression of osteoarthritis. Arthritis Rheum 56: 3670-3674, 2007.