Nanomolar to sub-picomolar affinity measurements of antibody-antigen interactions and protein multimerizations: Fluorescence-assisted high-performance liquid chromatography

Analytical Biochemistry

Volumn 437, Issue 2, 2013, Pages 118-122

  Rispens, Theo ^a   Heer, Pleuni Ooijevaar De ^b   Derksen, Ninotska I L ^b   Wolbink, Gertjan ^b   Van Schouwenburg, Pauline A ^b   Kruithof, Simone ^b   Aalberse, Rob C ^b  

^a SANQUIN RESEARCH   (Netherlands)

^b UNIVERSITY OF AMSTERDAM   (Netherlands)

Author keywords

Binding assay; Dissociation constant; High affinity; High performance liquid chromatography

Indexed keywords

ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; DISSOCIATION; FLUORESCENCE; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MONOCLONAL ANTIBODIES; SURFACE PLASMON RESONANCE;

AFFINITY MEASUREMENT; ANTIBODY-ANTIGEN INTERACTIONS; BINDING ASSAYS; DISSOCIATION CONSTANT; FLUORESCENCE DETECTION; HIGH AFFINITY; OPTIMAL CONDITIONS; SENSITIVE ASSAY;

SIZE EXCLUSION CHROMATOGRAPHY;

ADALIMUMAB; MONOCLONAL ANTIBODY;

ANTIGEN ANTIBODY REACTION; ARTICLE; BINDING AFFINITY; FLUORESCENCE; GEL PERMEATION CHROMATOGRAPHY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HUMAN; MOLECULAR CLONING; PRIORITY JOURNAL; PROTEIN MULTIMERIZATION; RHEUMATOID ARTHRITIS; SENSITIVITY ANALYSIS; SURFACE PLASMON RESONANCE;

EID: 84875983163     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2013.02.027     Document Type: Article

References (17)

1. B. Luginbuhl, Z. Kanyo, R.M. Jones, R.J. Fletterick, S.B. Prusiner, F.E. Cohen, R.A. Williamson, D.R. Burton, and A. Pluckthun Directed evolution of an anti-prion protein scFv fragment to an affinity of 1 pM and its structural interpretation J. Mol. Biol. 363 2006 75 97 (Pubitemid 44414837)
2. D. Shealy, A. Cai, K. Staquet, A. Baker, E.R. Lacy, L. Johns, O. Vafa, G. Gunn III, S. Tam, S. Sague, D. Wang, M. Brigham-Burke, P. Dalmonte, E. Emmell, B. Pikounis, P.J. Bugelski, H. Zhou, B. Scallon, and J. Giles-Komar Characterization of golimumab, a human monoclonal antibody specific for human tumor necrosis factor α MAbs 2 2010 428 439
3. C. Votsmeier, H. Plittersdorf, O. Hesse, A. Scheidig, M. Strerath, U. Gritzan, K. Pellengahr, P. Scholz, A. Eicker, D. Myszka, W.M. Coco, and U. Haupts Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display MAbs 4 2012 341 348
4. C. Boozer, G. Kim, S. Cong, H. Guan, and T. Londergan Looking towards label-free biomolecular interaction analysis in a high-throughput format: a review of new surface plasmon resonance technologies Curr. Opin. Biotechnol. 17 2006 400 405 (Pubitemid 44163461)
5. T. Rispens, V.H. Te, P. Hemker, H. Speijer, W. Hermens, and L. Aarden Label-free assessment of high-affinity antibody-antigen binding constants: comparison of bioassay, SPR, and PEIA-ellipsometry J. Immunol. Methods 365 2011 50 57
6. A.W. Drake, D.G. Myszka, and S.L. Klakamp Characterizing high-affinity antigen/antibody complexes by kinetic- and equilibrium-based methods Anal. Biochem. 328 2004 35 43 (Pubitemid 38479615)
7. K. High, Y. Meng, M.W. Washabaugh, and Q. Zhao Determination of picomolar equilibrium dissociation constants in solution by enzyme-linked immunosorbent assay with fluorescence detection Anal. Biochem. 347 2005 159 161 (Pubitemid 41608581)
8. J. Piehler, A. Brecht, T. Giersch, B. Hock, and G. Gauglitz Assessment of affinity constants by rapid solid phase detection of equilibrium binding in a flow system J. Immunol. Methods 201 1997 189 206 (Pubitemid 27086716)
9. G.A. Canziani, J.A. Melero, and E.R. Lacy Characterization of neutralizing affinity-matured human respiratory syncytial virus F binding antibodies in the sub-picomolar affinity range J. Mol. Recognit. 25 2012 136 146
10. Z. Kaymakcalan, P. Sakorafas, S. Bose, S. Scesney, L. Xiong, D.K. Hanzatian, J. Salfeld, and E.H. Sasso Comparisons of affinities, avidities, and complement activation of adalimumab, infliximab, and etanercept in binding to soluble and membrane tumor necrosis factor Clin. Immunol. 131 2009 308 316
11. P. Rathanaswami, S. Roalstad, L. Roskos, Q.J. Su, S. Lackie, and J. Babcook Demonstration of an in vivo generated sub-picomolar affinity fully human monoclonal antibody to interleukin-8 Biochem. Biophys. Res. Commun. 334 2005 1004 1013 (Pubitemid 41074676)
12. P.A. van Schouwenburg, L.A. van de Stadt, R.N. de Jong, E.E. van Buren, S. Kruithof, G.E. de, M. Hart, S.M. van Ham, T. Rispens, L. Aarden, G.J. Wolbink, and D. Wouters Adalimumab elicits a restricted anti-idiotypic antibody response in autoimmune patients resulting in functional neutralization Ann. Rheum. Dis. 72 2013 104 109
13. T. Rispens, H.P. Ooijevaar-de, O. Bende, and R.C. Aalberse Mechanism of immunoglobulin G4 Fab-arm exchange J. Am. Chem. Soc. 133 2011 10302 10311
14. C.N. Gallagher, and R.E. Huber Monomer-dimer equilibrium of uncomplemented M15 β-galactosidase from Escherichia coli Biochemistry 36 1997 1281 1286 (Pubitemid 27074951)
15. D.J. Winzor From gel filtration to biosensor technology: the development of chromatography for the characterization of protein interactions J. Mol. Recognit. 13 2000 279 298
16. J. Foote, and H.N. Eisen Kinetic and affinity limits on antibodies produced during immune responses Proc. Natl. Acad. Sci. USA 92 1995 1254 1256
17. J. Foote, and H.N. Eisen Breaking the affinity ceiling for antibodies and T cell receptors Proc. Natl. Acad. Sci. USA 97 2000 10679 10681