Femtomolar Fab binding affinities to a protein target by alternative CDR residue co-optimization strategies without phage or cell surface display

mAbs

Volumn 4, Issue 3, 2012, Pages 341-348

  Votsmeier, Christian ^a   Plittersdorf, Hanna ^a   Hesse, Oliver ^a   Scheidig, Andreas ^a   Strerath, Michael ^a   Gritzan, Uwe ^a   Pellengahr, Klaus ^a   Scholz, Peter ^a   Eicker, Andrea ^a   Myszka, David ^b   Coco, Wayne M ^a   Haupts, Ulrich ^a  

^a BAYER PHARMA AG   (Germany)

^b Biosensor Tools LLC   (United States)

Author keywords

Adalimumab; Affinity maturation; Antibody; Compartmentalized screening; Directed evolution; Femtomolar; High throughput screening; HTS ELISA assays; HTS TR FRET assays; Humira; In vitro recombination; Phage display; Protein protein interactions; Saturation mutagenesis; Yeast display

Indexed keywords

ADALIMUMAB; IMMUNOGLOBULIN F(AB) FRAGMENT; TUMOR NECROSIS FACTOR ANTIBODY;

ANTIBODY AFFINITY; ARTICLE; BINDING AFFINITY; CELL SURFACE; COMPLEMENTARITY DETERMINING REGION; DRUG RECEPTOR BINDING; GENE TECHNOLOGY; HIGH THROUGHPUT SCREENING; MOLECULAR LIBRARY; MUTAGENESIS; NONHUMAN; QUANTITATIVE ANALYSIS;

ANTIBODY AFFINITY; CELL SURFACE DISPLAY TECHNIQUES; COMPLEMENTARITY DETERMINING REGIONS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOGLOBULIN FAB FRAGMENTS; MUTAGENESIS, SITE-DIRECTED; MUTATION; PROTEIN BINDING;

EID: 84860903776     PISSN: 19420862     EISSN: 19420870     Source Type: Journal    
DOI: 10.4161/mabs.19981     Document Type: Article

References (26)

1. Reichert JM. Metrics for antibody therapeutics development. MAbs 2010; 2:695-700; PMID:20930555; http://dx.doi.org/10.4161/mabs.2.6.13603.
2. Lerner RA. Manufacturing immunity to disease in a test tube: the magic bullet realized. Angew Chem Int Ed Engl 2006; 45:8106-25; PMID:17120282; http://dx.doi.org/10.1002/anie.200603381.
3. Boder ET, Midelfort KS, Wittrup KD. Directed evolution of antibody fragments with monovalent femtomolar antigen-binding affinity. Proc Natl Acad Sci USA 2000; 97:10701-5; PMID:10984501; http://dx.doi.org/10.1073/pnas. 170297297.
4. Steidl S, Ratsch O, Brocks B, Dürr M, Thomassen-Wolf E. In vitro affinity maturation of human GM-CSF antibodies by targeted CDR-diversification. Mol Immunol 2008; 46:135-44; PMID:18722015; http://dx.doi.org/10.1016/j.molimm. 2008.07.013.
5. Hoogenboom HR. Selecting and screening recombinant antibody libraries. Nat Biotechnol 2005; 23:1105-16; PMID:16151404; http://dx.doi.org/10.1038/ nbt1126.
6. Kay BK, Adey NB, He YS, Manfredi JP, Mataragnon AH, Fowlkes DM. An M13 phage library displaying random 38-amino-acid peptides as a source of novel sequences with affinity to selected targets. Gene 1993; 128:59-65; PMID:8508960; http://dx.doi.org/10.1016/0378-1119(93)90153-T.
7. Clackson T, Wells JA. In vitro selection from protein and peptide libraries. Trends Biotechnol 1994; 12:173-84; PMID:7764900; http://dx.doi.org/ 10.1016/0167-7799(94)90079-5.
8. Derda R, Tang SK, Li SC, Ng S, Matochko W, Jafari MR. Diversity of phage-displayed libraries of peptides during panning and amplification. Molecules 2011; 16:1776-803; PMID:21339712; http://dx.doi.org/10.3390/ molecules16021776.
9. Krebber A, Burmester J, Plückthun A. Inclusion of an upstream transcriptional terminator in phage display vectors abolishes background expression of toxic fusions with coat protein g3p. Gene 1996; 178:71-4; PMID:8921894; http://dx.doi.org/10.1016/0378-1119(96)00337-X.
10. Bain B, Brazil M. Adalimumab. Nat Rev Drug Discov 2003; 2:693-4; PMID:12953696; http://dx.doi.org/10.1038/nrd1182.
11. Osbourn J, Groves M, Vaughan T. From rodent reagents to human therapeutics using antibody guided selection. Methods 2005; 36:61-8; PMID:15848075; http://dx.doi.org/10.1016/j.ymeth.2005.01.006.
12. Rajpal A, Beyaz N, Haber L, Cappuccilli G, Yee H, Bhatt RR, et al. A general method for greatly improving the affinity of antibodies by using combinatorial libraries. Proc Natl Acad Sci USA 2005; 102:8466-71; PMID:15939870; http://dx.doi.org/10.1073/pnas.0503543102.
13. Salfeld JG, Allen DJ, Hoogenboom HR, Kaymakcalan Z, Labkovsky B, Mankovich JA, et al. Human antibodies that bind human TNFα. US Patent 2003; 6090382.
14. Kabat EA, Wu TT, Reid-Miller M, Perry H, Gottesman K. Sequences of Proteins of Immunological Interest, US Govt. Printing Off 1987; 4:165-492.
15. Al-Lazikani B, Lesk AM, Chothia C. Standard conformations for the canonical structures of immunoglobulins. J Mol Biol 1997; 273:927-48; PMID:9367782; http://dx.doi.org/10.1006/jmbi.1997.1354.
16. Web Antibody Modeling. http://antibody.bath.ac.uk
17. MacCallum RM, Martin AC, Thornton JM. Antibody-antigen interactions: contact analysis and binding site topography. J Mol Biol 1996; 262:732-45; PMID:8876650; http://dx.doi.org/10.1006/jmbi.1996.0548.
18. Jansson B, Uhlén M, Nygren PA. All individual domains of staphylococcal protein A show Fab binding. FEMS Immunol Med Microbiol 1998; 20:69-78; PMID:9514577; http://dx.doi.org/10.1016/S0928-8244(97)00108-9.
19. Wark KL, Hudson PJ. Latest technologies for the enhancement of antibody affinity. Adv Drug Deliv Rev 2006; 58:657-70; PMID:16828920; http://dx.doi.org/10.1016/j.addr.2006.01.025.
20. Foote J, Eisen HN. Kinetic and affinity limits on antibodies produced during immune responses. Proc Natl Acad Sci USA 1995; 92:1254-6; PMID:7877964; http://dx.doi.org/10.1073/pnas.92.5.1254.
21. Jermutus L, Honegger A, Schwesinger F, Hanes J, Plückthun A. Tailoring in vitro evolution for protein affinity or stability. Proc Natl Acad Sci USA 2001; 98:75-80; PMID:11134506; http://dx.doi.org/10.1073/pnas.011311398.
22. Hanes J, Plückthun A. In vitro selection and evolution of functional proteins by using ribosome display. Proc Natl Acad Sci USA 1997; 94:4937-42; PMID:9144168; http://dx.doi.org/10.1073/pnas.94.10.4937.
23. Wu H. Simultaneous humanization and affinity optimization of monoclonal antibodies. Methods Mol Biol 2003; 207:197-212; PMID:12412476.
24. Santora LC, Kaymakcalan Z, Sakorafas P, Krull IS, Grant K. Characterization of noncovalent complexes of recombinant human monoclonal antibody and antigen using cation exchange, size exclusion chromatography and BIAcore. Anal Biochem 2001; 299:119-29; PMID:11730333; http://dx.doi.org/10. 1006/abio.2001.5380.
25. Kwong KY, Rader C. E. coli Expression and Purification of Fab Antibody Fragments. Curr Protoc Protein Sci 2009; 6:10.
26. Krippner-Heidenreich A, Tübing F, Bryde S, Willi S, Zimmermann G, Scheurich P. Control of receptor-induced signaling complex formation by the kinetics of ligand/receptor interaction. J Biol Chem 2002; 277:44155-63; PMID:12215450; http://dx.doi.org/10.1074/jbc.M207399200.