Proteomics based detection of differentially expressed proteins in human osteoblasts subjected to mechanical stress

Biochemistry and Cell Biology

Volumn 91, Issue 2, 2013, Pages 109-115

  Li, Fei Fei ^a   Chen, Fu Lin ^b   Wang, Huan ^a   Yu, Shi Bin ^a   Cui, Ji Hong ^b   Ding, Yin ^a   Feng, Xue ^a  

^a FOURTH MILITARY MEDICAL UNIVERSITY   (China)

^b NORTHWEST UNIVERSITY   (China)

Author keywords

Bone; Mechanical loading; Mechanotransduction; Osteoblasts; Proteomics

Indexed keywords

2-D ELECTROPHORESIS; EUKARYOTIC INITIATION FACTOR; MALDI-TOF MASS SPECTROSCOPY; MECHANICAL LOADING; MECHANOTRANSDUCTION; PEPTIDYL-PROLYL ISOMERASE; PROTEIN EXPRESSION PROFILES; PROTEOMICS;

BIOINFORMATICS; BONE; CELL PROLIFERATION; CELLS; CYTOLOGY; ELECTROPHORESIS; MECHANISMS; MOLECULAR BIOLOGY; OSTEOBLASTS; STRESS ANALYSIS;

PROTEINS;

CYCLOPHILIN A; INITIATION FACTOR 2; LACTATE DEHYDROGENASE; PROTON TRANSPORTING ADENOSINE TRIPHOSPHATE SYNTHASE; UBIQUITIN THIOLESTERASE;

ARTICLE; BIOINFORMATICS; BONE DEVELOPMENT; CELL PROLIFERATION; CONTROLLED STUDY; DOWN REGULATION; ENERGY METABOLISM; HUMAN; HUMAN CELL; INTRACELLULAR SIGNALING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MECHANICAL STRESS; MECHANOTRANSDUCTION; OSTEOBLAST; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEOMICS; STRESS; TWO DIMENSIONAL ELECTROPHORESIS; UPREGULATION;

CELL LINE; ENERGY METABOLISM; GENE EXPRESSION PROFILING; GENE EXPRESSION REGULATION; HUMANS; MECHANOTRANSDUCTION, CELLULAR; OSTEOBLASTS; OSTEOGENESIS; PROTEINS; PROTEOMICS; STRESS, MECHANICAL; STRESS, PHYSIOLOGICAL;

EUKARYOTA;

EID: 84875786768     PISSN: 08298211     EISSN: 12086002     Source Type: Journal    
DOI: 10.1139/bcb-2012-0021     Document Type: Article

References (46)

1. Alldridge L.C. Harris H.J. Plevin R. Hannon R. Bryant C.E. 1999. The annexin protein lipocortin 1 regulates the MAPK/ERK pathway. J. Biol. Chem. 274 (53): 37620-37628. 10.1074/jbc.274.53.37620. 10608817.
2. Baik A.D. Lu X.L. Qiu J. Huo B. Hillman E.M. Dong C. Guo X.E. 2010. Quasi-3D cytoskeletal dynamics of osteocytes under fluid flow. Biophys. J. 99 (9): 2812-2820. 10.1016/j.bpj.2010.08.064. 21044578.
3. Bastow E.R. Byers S. Golub S.B. Clarkin C.E. Pitsillides A.A. Fosang A.J. 2008. Hyaluronan synthesis and degradation in cartilage and bone. Cell. Mol. Life Sci. 65 (3): 395-413. 10.1007/s00018-007-7360-z. 17965830.
4. Bhatt K.A. Chang E.I. Warren S.M. Lin S.E. Bastidas N. Ghali S. et al. 2007. Uniaxial mechanical strain: an in vitro correlate to distraction osteogenesis. J. Surg. Res. 143 (2): 329-336. 10.1016/j.jss.2007.01.023. 17950332.
5. Byers B.A. Pavlath G.K. Murphy T.J. Karsenty G. Garcia A.J. 2002. Cell-type-dependent up-regulation of in vitro mineralization after overexpression of the osteoblast-specific transcription factor Runx2/Cbfal. J. Bone Miner. Res. 17 (11): 1931-1944. 12412799.
6. Chen A.P. Wang G.L. Qu Z.L. Lu C.X. Liu N. Wang F. Xia G.X. 2007. Ectopic expression of ThCYP1, a stress-responsive cyclophilin gene from Thellungiella halophila, confers salt tolerance in fission yeast and tobacco cells. Plant Cell Rep. 26 (2): 237-245. 10.1007/s00299-006-0238-y. 16972091.
7. Corbett J.M. Dunn M.J. Posch A. Görg A. 1994. Positional reproducibility of protein spots in two-dimensional polyacrylamide gel electrophoresis using immobilised pH gradient isoelectric focusing in the first dimension: an interlaboratory comparison. Electrophoresis, 15 (8-9): 1205-1211. 10.1002/elps.11501501182. 7532129.
8. Costessi A. Pines A., D' Andrea P. Romanello M. Damante G. Cesaratto L. et al. 2005. Extracellular nucleotides activate Runx2 in the osteoblast-like HOBIT cell line: a possible molecular link between mechanical stress and osteoblasts' response. Bone. 36 (3): 418-432. 10.1016/j.bone.2004.10.016. 15777650.
9. Delorme V. Machacek M. DerMardirossian C. Anderson K.L. Wittmann T. Hanein D. et al. 2007. Cofilin activity downstream of Pak1 regulates cell protrusion efficiency by organizing lamellipodium and lamella actin networks. Dev. Cell, 13 (5): 646-662. 10.1016/j.devcel.2007.08.011. 17981134.
10. Devin A. Rigoulet M. 2007. Mechanisms of mitochondrial response to variations in energy demand in eukaryotic cells. Am. J. Physiol. Cell Physiol. 292 (1): C52-C58. 10.1152/ajpcell.00208.2006. 16943247.
11. elHaj A.J. Walker L.M. Preston M.R. Publicover S.J. 1999. Mechanotransduction pathways in bone: calcium fluxes and the role of voltage-operated calcium channels. Med. Biol. Eng. Comput. 37 (3): 403-409. 10.1007/BF02513320. 10505395.
12. Feister H.A. Onyia J.E. Miles R.R. Yang X. Galvin R. Hock J.M. Bidwell J.P. 2000. The expression of the nuclear matrix proteins NuMA, topoisomerase II-alpha, and-beta in bone and osseous cell culture: regulation by parathyroid hormone. Bone, 26 (3): 227-234. 10709994.
13. Furukawa K. 2006. Current topics in pharmacological research on bone metabolism: molecular basis of ectopic bone formation induced by mechanical stress. J. Pharmacol. Sci. 100 (3): 201-204. 10.1254/jphs.FMJ05004X4. 16518075.
14. Gharbi M. Deberg M. Henrotin Y. 2011. Application for proteomic techniques in studying osteoarthritis: a review. Front. Physiol. 2: 90. 10.3389/fphys.2011.00090. 22144964.
15. Giorgianni F. Desiderio D.M. Beranova-Giorgianni S. 2003. Proteome analysis using isoelectric focusing in immobilized pH gradient gels followed by mass spectrometry. Electrophoresis, 24 (1-2): 253-259. 10.1002/elps.200390021. 12652597.
16. Hens J.R. Wilson K.M. Dann P. Chen X. Horowitz M.C. Wysolmerski J.J. 2005. TOPGAL mice show that the canonical Wnt signaling pathway is active during bone development and growth and is activated by mechanical loading in vitro. J. Bone Miner. Res. 20 (7): 1103-1113. 10.1359/JBMR.050210. 15940363.
17. Imai Y. Kondoh S. Kouzmenko A. Kato S. 2003. Regulation of bone metabolism by nuclear receptors. Mol. Cell. Endocrinol. 310 (1-2): 3-10. 10.1016/j.mce.2008.08.015. 18789372.
18. Inoue M. Tanaka H. Moriwake T. Oka M. Sekiguchi C. Seino Y. 2000. Altered biochemical markers of bone turnover in humans during 120 days of bed rest. Bone, 26 (3): 281-286. 10.1016/S8756-3282(99)00282-3. 10710002.
19. Inoue D. Kido S. Matsumoto T. 2004. Transcriptional induction of FosB/DeltaFosB gene by mechanical stress in osteoblasts. J. Biol. Chem. 279 (48): 49795-49803. 15383527.
20. Jiang Y. Liu H. Li H. Wang F. Cheng K Zhou G. et al. 2011. A proteomic analysis of engineered tendon formation under dynamic mechanical loading in vitro. Biomaterials, 32 (17): 4085-4095. 10.1016/j.biomaterials.2011.02.033. 21402406.
21. Kaempfer R. Rosen H. Israeli R. 1978. Translational control: recognition of the methylated 5′ end and an internal sequence in eukaryotic mRNA by the initiation factor that binds methionyl-tRNAfMet. Proc. Natl. Acad. Sci. U.S.A. 75 (2): 650-654. 10.1073/pnas.75.2.650. 273228.
22. Kapur S. Baylink D.J. Lau K.H. 2003. Fluid flow shear stress stimulates human osteoblast proliferation and differentiation through multiple interacting and competing signal transduction pathways. Bone, 32 (3): 241-251. 10.1016/S8756-3282(02)00979-1. 12667551.
23. Kido S. Kuriwaka-Kido R. Imamura T. Ito Y. Inoue D. Matsumoto T. 2009. Mechanical stress induces Interleukin-11 expression to stimulate osteoblast differentiation. Bone, 45 (6): 1125-1132. 10.1016/j.bone.2009.07.087. 19665600.
24. Kido S. Kuriwaka-Kido R. Umino-Miyatani Y. Endo I. Inoue D. Taniguchi H. et al. 2010. Mechanical stress activates Smad pathway through PKCdelta to enhance interleukin-11 gene transcription in osteoblasts. PLoS One, 5 (9): e13090. 10.1371/journal.pone.0013090. 20927330.
25. Kitching R. Qi S. Li V. Raouf A. Vary C.P. Seth A. 2002. Coordinate gene expression patterns during osteoblast maturation and retinoic acid treatment of MC3T3-E1 cells. J. Bone Miner. Metab. 20 (5): 269-280. 10.1007/s007740200039. 12203032.
26. Lean J.M. Mackay A.G. Chow J.W. Chambers T.J. 1996. Osteocytic expression of mRNA for c-fos and IGF-I: an immediate early gene response to an osteogenic stimulus. Am. J. Physiol. 270 (6): E937-E945. 8764176.
27. Liu D. Genetos D.C. Shao Y. Geist D.J. Li J. Ke H.Z. et al. 2008. Activation of extracellular-signal regulated kinase (ERK1/2) by fluid shear is Ca(2+)-and ATP-dependent in MC3T3-E1 osteoblasts. Bone, 42 (4): 644-652. 10.1016/j.bone.2007.09.058. 18291742.
28. Malone A.M. Batra N.N. Shivaram G. Kwon R.Y. You L. Kim C.H. et al. 2007. The role of actin cytoskeleton in oscillatory fluid flow-induced signaling in MC3T3-E1 osteoblasts. Am. J. Physiol. Cell Physiol. 292 (5): C1830-C1836. 10.1152/ajpcell.00352.2005. 17251324.
29. Miles R.R. Turner C.H. Santerre R. Tu Y. McClelland P. Argot J. et al. 1998. Analysis of differential gene expression in rat tibia after an osteogenic stimulus in vivo: mechanical loading regulates osteopontin and myeloperoxidase. J. Cell. Biochem. 68 (3): 355-365. 10.1002/(SICI)1097-4644(19980301)68:3<355: :AID-JCB6>>3.0.CO;2-T. 9518261.
30. Mitsui N. Suzuki N. Maeno M. Yanagisawa M. Koyama Y. Otsuka K. Shimizu N. 2006. Optimal compressive force induces bone formation via increasing bone morphogenetic proteins production and decreasing their antagonists production by Saos-2 cells. Life Sci. 78 (23): 2697-2706. 10.1016/j.lfs.2005.10.024. 16337660.
31. Morey E.R. Baylink D.J. 1978. Science, 201 (4361): 1138-1141. 10.1126/science.150643. 150643.
32. Rescher U. Gerke V. 2004. Annexins-unique membrane binding proteins with diverse functions. J. Cell Sci. 117 (3): 2631-2639. 10.1242/jcs.01245. 15169834.
33. Rezzonico R. Cayatte C. Bourget-Ponzio I. Romey G. Belhacene N. Loubat A. et al. 2003. Focal adhesion kinase pp125FAK interacts with the large conductance calcium-activated hSlo potassium channel in human osteoblasts: potential role in mechanotransduction. J. Bone Miner. Res. 18 (10): 1863-1871. 10.1359/jbmr.2003.18.10.1863. 14584897.
34. Robinson J.A. Chatterjee-Kishore M. Yaworsky P.J. Cullen D.M. Zhao W. Li C. et al. 2006. Wnt/beta-catenin signaling is a normal physiological response to mechanical loading in bone. J. Biol. Chem. 281 (42): 31720-31728. 10.1074/jbc.M602308200. 16908522.
35. Romanello M. Pani B. Bicego M., and D' Andrea P. 2001. Mechanically induced ATP release from human osteoblastic cells. Biochem. Biophys. Res. Commun. 289 (5): 1275-1281. 10.1006/bbrc.2001.6124. 11741333.
36. Saito A. Ochiai K. Kondo S. Tsumagari K. Murakami T. Cavener D.R. Imaizumi K. 2011. Endoplasmic reticulum stress response mediated by the PERK-eIF2(alpha)-ATF4 pathway is involved in osteoblast differentiation induced by BMP2. J. Biol. Chem. 286 (6): 4809-4818. 21135100.
37. Sakai D. Kii I. Nakagawa K. Matsumoto H.N. Takahashi M. Yoshida S. et al. 2011. Remodeling of actin cytoskeleton in mouse periosteal cells under mechanical loading induces periosteal cell proliferation during bone formation. PLoS One, 6 (9): e24847. 10.1371/journal.pone.0024847. 21935480.
38. Senior A.E. 2007. ATP synthase: motoring to the finish line. Cell, 130 (2): 220-221. 10.1016/j.cell.2007.07.004. 17662937.
39. Shakibaei M. John T. Schulze-Tanzil G. Lehmann I. Mobasheri A. 2007. Suppression of NF-kappaB activation by curcumin leads to inhibition of expression of cyclo-oxygenase-2 and matrix metalloproteinase-9 in human articular chondrocytes: Implications for the treatment of osteoarthritis. Biochem. Pharmacol. 73 (9): 1434-1445. 10.1016/j.bcp.2007.01.005. 17291458.
40. Tang L. Lin Z. Li Y.M. 2006. Effects of different magnitudes of mechanical strain on osteoblasts in vitro. Biochem. Biophys. Res. Commun. 344 (1): 122-128. 10.1016/j.bbrc.2006.03.123. 16603128.
41. Vorum H. Østergaard M. Hensechke P. Enghild J.J. Riazati M. Rice G.E. 2004. Proteomic analysis of hyperoxia-induced responses in the human choriocarcinoma cell line JEG-3. Proteomics, 4 (3): 861-867. 10.1002/pmic.200300639. 14997506.
42. Watanabe Y. Ohshima H. Mizuno K. Sekiguchi C. Fukunaga M. Kohri K. et al. 2004. Intravenous pamidronate prevents femoral bone loss and renal stone formation during 90-day bed rest. J. Bone Miner. Res. 19 (11): 1771-1778. 10.1359/JBMR.040811. 15476576.
43. Wei J. Sheng X. Feng D. McGrath B. Cavener D.R. 2008. PERK is essential for neonatal skeletal development to regulate osteoblast proliferation and differentiation. J. Cell. Physiol. 217 (3): 693-707. 10.1002/jcp.21543. 18683826.
44. Yang Y. Lu N. Zhou J. Chen Z.N. Zhu P. 2008. Cyclophilin A up-regulates MMP-9 expression and adhesion of monocytes/macrophages via CD147 signalling pathway in rheumatoid arthritis. Rheumatology (Oxford), 47 (9): 1299-1310. 10.1093/rheumatology/ken225. 18567920.
45. Zhang W.B. Wang L. 2009. Label-free quantitative proteome analysis of skeletal tissues under mechanical load. J. Cell. Biochem. 108 (3): 600-611. 10.1002/jcb.22291. 19670388.
46. Zhao X. Chao Y. Chen P. Tang Y. Liu D. Su P. Cui X. 2011. hRpn13, a newly identified component of the 19S particle, regulates proliferation, differentiation, and function in the human osteoblast-like cell line MG63: Role of hRpn13 in osteoblasts. J. Physiol. Biochem. 10.1007/s13105-011-0126-y. 22057889.