Aminolaevulinic acid synthase of rhodobacter capsulatus: High-resolution kinetic investigation of the structural basis for substrate binding and catalysis

Biochemical Journal

Volumn 451, Issue 2, 2013, Pages 205-216

  Kaufholz, Anna Lena ^a   Hunter, Gregory A ^b   Ferreira, Gloria C ^b   Lendrihas, Thomas ^b   Hering, Vanessa ^a   Layer, Gunhild ^a   Jahn, Martina ^a   Jahn, Dieter ^a  

^a TECHNICAL UNIVERSITY OF BRAUNSCHWEIG   (Germany)

^b UNIVERSITY OF SOUTH FLORIDA   (United States)

Author keywords

5 aminolaevulinic acid synthase (ALAS); Haem biosynthesis; Quinonoid intermediate formation; Steady state kinetics; Stopped flow spectroscopy

Indexed keywords

ALANINE; AMINO ACID; AMINOLAEVULINIC ACID SYNTHASE; AMINOLEVULINIC ACID; BACTERIAL ENZYME; GENOMIC DNA; QUINONE DERIVATIVE; STRUCTURAL PROTEIN; SUCCINYL COENZYME A; SYNTHETASE; UNCLASSIFIED DRUG;

ABSORPTION SPECTROSCOPY; AMINO ACID SUBSTITUTION; ARTICLE; CONFORMATIONAL TRANSITION; CRYSTAL STRUCTURE; DECARBOXYLATION; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; HEME SYNTHESIS; HYDROGEN BOND; HYDROPHOBICITY; MOLECULAR RECOGNITION; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN PURIFICATION; PROTON TRANSPORT; RHODOBACTER CAPSULATUS; SITE DIRECTED MUTAGENESIS; STEADY STATE; TURNOVER TIME;

5-AMINOLEVULINATE SYNTHETASE; ACYL COENZYME A; ARGININE; BACTERIAL PROTEINS; CATALYSIS; CATALYTIC DOMAIN; GLYCINE; HYDROGEN-ION CONCENTRATION; KINETICS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; RHODOBACTER CAPSULATUS; SUBSTRATE SPECIFICITY; THREONINE;

RHODOBACTER CAPSULATUS;

EID: 84875628518     PISSN: 02646021     EISSN: 14708728     Source Type: Journal    
DOI: 10.1042/BJ20121041     Document Type: Article

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