Three-dimensional structure of 2-amino-3-ketobutyrate CoA ligase from Escherichia coli complexed with a PLP-substrate intermediate: Inferred reaction mechanism

Biochemistry

Volumn 40, Issue 17, 2001, Pages 5151-5160

  Schmidt, A ^a   Sivaraman, J ^a   Li, Y ^a   Larocque, R ^a   Barbosa, J A R G ^a   Smith, C ^a   Matte, A ^a   Schrag, J D ^a   Cygler, M ^a  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; DEHYDROGENATION; DIMERS; ELECTRON DENSITY MEASUREMENT; ESCHERICHIA COLI; METABOLISM; PURIFICATION;

THREE DIMENSIONAL STRUCTURES;

ENZYME KINETICS;

2 AMINO 3 KETOBUTYRATE COENZYME A LIGASE; 8 AMINO 7 OXONONANOATE SYNTHASE; ACETYL COENZYME A; ASPARTATE AMINOTRANSFERASE; BACTERIAL ENZYME; OXIDOREDUCTASE; PYRIDOXAL 5 PHOSPHATE; SCHIFF BASE; THREONINE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; CATALYSIS; COVALENT BOND; CRYSTALLIZATION; DEGRADATION; ENZYME ACTIVE SITE; ENZYME MECHANISM; ENZYME SPECIFICITY; ENZYME STRUCTURE; ENZYME SUBSTRATE COMPLEX; ESCHERICHIA COLI; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PURIFICATION;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA;

EID: 0035340280     PISSN: 00062960     EISSN: None     Source Type: Journal    
DOI: 10.1021/bi002204y     Document Type: Article

References (57)

1. The CCP4 suite: Programs for protein crystallography