HOPE-preservation of paraffin-embedded sputum samples-A new way of bioprofiling in COPD

Respiratory Medicine

Volumn 107, Issue 4, 2013, Pages 587-595

  Pedersen, Frauke ^a,c   Marwitz, Sebastian ^b,c   Seehase, Sophie ^b,c   Kirsten, Anne Marie ^a,c   Zabel, Peter ^b,c   Vollmer, Ekkehard ^b,c   Rabe, Klaus F ^a,c   Magnussen, Helgo ^a,c   Watz, Henrik ^a,c   Goldmann, Torsten ^b,c  

^a KRANKENHAUS GROSSHANSDORF   (Germany)

^b RESEARCH CENTER BORSTEL   (Germany)

^c German Center for Lung Research   (Germany)

Author keywords

Biobanking; Immunocytochemistry; Induced sputum; Morphology; RNA; Transcriptome

Indexed keywords

BIOLOGICAL MARKER; CD68 ANTIGEN; CD8 ANTIGEN; GELATINASE B; INTERLEUKIN 17; INTERLEUKIN 8; MYELOPEROXIDASE; RNA; TRANSCRIPTOME;

ADULT; AGED; ARTICLE; CHRONIC OBSTRUCTIVE LUNG DISEASE; CLINICAL ARTICLE; CONTROLLED STUDY; DISEASE SEVERITY; FEMALE; HEPES GLUTAMIC ACID BUFFER MEDIATED ORGANIC SOLVENT PROTECTION EFFECT TECHNIQUE; HUMAN; IMMUNOCYTOCHEMISTRY; LABORATORY TEST; MALE; MICROARRAY ANALYSIS; PRIORITY JOURNAL; RNA TRANSCRIPTION; SMOKING; SPUTUM ANALYSIS; TRANSCRIPTOMICS;

AGED; BIOLOGICAL MARKERS; BIOLOGICAL SPECIMEN BANKS; CELL COUNT; FEMALE; FORCED EXPIRATORY VOLUME; GENE EXPRESSION PROFILING; HEPES; HUMANS; INFLAMMATION MEDIATORS; MALE; MIDDLE AGED; OLIGONUCLEOTIDE ARRAY SEQUENCE ANALYSIS; PARAFFIN EMBEDDING; PULMONARY DISEASE, CHRONIC OBSTRUCTIVE; SMOKING; SPUTUM; TISSUE FIXATION;

EID: 84875266210     PISSN: 09546111     EISSN: 15323064     Source Type: Journal    
DOI: 10.1016/j.rmed.2012.12.006     Document Type: Article

References (26)

1. H. Magnussen, O. Holz, P.J. Sterk, and F.E. Hargreave Noninvasive methods to measure airway inflammation: future considerations Eur Respir J 16 2000 1175 1179
2. B. Nicholas, and R. Djukanovic Induced sputum: a window to lung pathology Biochem Soc Trans 37 2009 868 872
3. S.D. Sagel, B.D. Wagner, M.M. Anthony, P. Emmett, and E.T. Zernanick Sputum biomarkers of inflammation and lung function decline in children with cystic fibrosis Am J Respir Crit Care Med 2012 10.1164/rccm.201203-0507OC
4. I.D. Pavord, P.J. Sterk, F.E. Hargreave, J.C. Kips, M.D. Inman, R. Louis, M.M. Pizzichini, E.H. Bel, I. Pin, D.C. Grootendorst, K. Parameswaran, and R. Djukanovic Clinical applications of assessment of airway inflammation using induced sputum Eur Respir J Suppl 37 2002 40s 43s
5. D.C. Grootendorst, S.A. Gauw, R.M. Verhoosel, P.J. Sterk, J.J. Hospers, D. Bredenbroker, T.D. Bethke, P.S. Hiemstra, and K.F. Rabe Reduction in sputum neutrophil and eosinophil numbers by the PDE4 inhibitor roflumilast in patients with COPD Thorax 62 12 2007 1081 1087
6. D. Singh, S.M. Fox, R. Tal-Singer, J. Plumb, S. Bates, P. Broad, J.H. Riley, and B. Celli Induced sputum genes associated with spirometric and radiological disease severity in COPD ex-smokers Thorax 66 6 2011 489 495
7. G.R. Van Pottelberge, P. Mestdagh, K.R. Bracke, O. Thas, Y.M. Durme, G.F. Joos, J. Vandesompele, and G.G. Brusselle MicroRNA expression in induced sputum of smokers and patients with chronic obstructive pulmonary disease Am J Respir Crit Care Med 183 7 2011 898 906
8. P. Prince, M. Bertrand, M.E. Boulay, M.C. Bernier, and L.P. Boulet Optimization of the conditions for preservation of induced sputum: influence of freezing on cellular analysis Chest 128 2005 980 985
9. J. Olert, K.H. Wiedorn, T. Goldmann, H. Kuhl, Y. Mehraein, H. Scherthan, F. Niketeghad, E. Vollmer, A.M. Müller, and J. Müller-Navia HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues Pathol Res Pract 197 2001 823 826
10. J. St-Laurent, M.E. Boulay, P. Prince, E. Bissonnette, and L.P. Boulet Comparison of cell fixation methods of induced sputum specimens: an immunocytochemical analysis J Immunol Meth 308 2006 36 42
11. T. Goldmann, K.H. Wiedorn, H. Kuhl, J. Olert, D. Branscheid, D. Pechkovsky, G. Zissel, J. Galle, J. Müller-Quernheim, and E. Vollmer Assessment of transcriptional gene activity in situ by application of HOPE-fixed, paraffin-embedded tissues Pathol Res Pract 198 2002 91 95
12. T. Goldmann, A.M. Flohr, H. Murua Escobar, B. Gerstmayer, U. Janssen, A. Bosio, S. Loeschke, E. Vollmer, and J. Bullerdiek The HOPE-technique permits Northern blot and microarray analyses in paraffin-embedded tissues Pathol Res Pract 200 2004 511 515
13. U. Uhlig, S. Uhlig, D. Branscheid, P. Zabel, E. Vollmer, and T. Goldmann HOPE technique enables Western blot analysis from paraffin-embedded tissues Pathol Res Pract 200 2004 469 472
14. O. Umland, A.J. Ulmer, E. Vollmer, and T. Goldmann HOPE fixation of cytospin preparations of human cells for in situ hybridization and immunocytochemistry J Histochem Cytochem 51 2003 977 980
15. T. Goldmann, E. Vollmer, and J. Gerdes What's cooking? detection of important biomarkers in HOPE-fixed, paraffin-embedded tissues eliminates the need for antigen retrieval Am J Pathol 163 2003 2638 2640
16. S. Marwitz, M. Abdullah, C. Vock, J.S. Fine, S. Visvanathan, K.I. Gaede, H.P. Hauber, P. Zabel, and T. Goldmann HOPE-BAL: improved molecular diagnostics by application of a novel technique for fixation and paraffin embedding J Histochem Cytochem 59 2011 601 614
17. O. Holz, R.A. Jorres, S. Koschyk, P. Speckin, L. Welker, and H. Magnussen Changes in sputum composition during sputum induction in healthy and asthmatic subjects Clin Exp Allergy 28 1998 284 292
18. J. Witchell, D. Varshney, T. Gajjar, A. Wangoo, and M. Goyal RNA isolation and quantitative PCR from HOPE- and formalin-fixed bovine lymph node tissues Pathol Res Pract 204 2008 105 111
19. T. Goldmann, D. Drömann, M. Marzouki, U. Schimmel, K. Debel, D. Branscheid, T. Zeiser, J. Rupp, J. Gerdes, P. Zabel, and E. Vollmer Tissue microarrays from HOPE-fixed specimens allow for enhanced high throughput molecular analyses in paraffin-embedded material Pathol Res Pract 201 2005 599 602
20. T. Goldmann, R. Burgemeister, U. Sauer, S. Loeschke, D.S. Lang, D. Branscheid, P. Zabel, and E. Vollmer Enhanced molecular analyses by combination of the HOPE-technique and laser microdissection Diagn Pathol 1 2006 2
21. G.C. Riise, S. Ahlstedt, S. Larsson, I. Enander, I. Jones, P. Larsson, and B. Andersson Bronchial inflammation in chronic bronchitis assessed by measurement of cell products in bronchial lavage fluid Thorax 50 1995 360 365
22. E. Pizzichini, M.M. Pizzichini, A. Efthimiadis, S. Evans, M.M. Morris, D. Squillace, G.J. Gleich, J. Dolovich, and F.E. Hargreave Indices of airway inflammation in induced sputum: reproducibility and validity of cell and fluid-phase measurements Am J Respir Crit Care Med 154 1996 308 317
23. P.G. Gibson, N. Saltos, and K. Fakes Acute anti-inflammatory effects of inhaled budesonide in asthma: a randomized controlled trial Am J Respir Crit Care Med 163 2001 32 36
24. O. Holz, J. Kips, and H. Magnussen Update on sputum methodology Eur Respir J 16 2000 355 359
25. T. Popov, R. Gottschalk, R. Kolendowicz, J. Dolovich, P. Powers, and F.E. Hargreave The evaluation of a cell dispersion method of sputum examination Clin Exp Allergy 24 1994 778 783
26. D. Darling, M. Tavassoli, M.H. Linskens, and F. Farzaneh DMSO induced modulation of c-myc steady-state RNA levels in a variety of different cell lines Oncogene 4 1989 175 179