The HOPE-technique permits Northern blot and microarray analyses in paraffin-embedded tissues

Pathology Research and Practice

Volumn 200, Issue 7-8, 2004, Pages 511-515

  Goldmann, Torsten ^a   Flohr, Aljoscha M ^b   Escobar, Hugo Murua ^b,c   Gerstmayer, Bernhard ^d   Janssen, Uwe ^d   Bosio, Andreas ^d   Loeschke, Siegfried ^b   Vollmer, Ekkehard ^a   Bullerdiek, Jörn ^b  

^a RESEARCH CENTER BORSTEL   (Germany)

^b UNIVERSITY OF BREMEN   (Germany)

^c UNIVERSITY OF VETERINARY MEDICINE HANNOVER   (Germany)

^d Memorec Biotec GmbH   (Germany)

Author keywords

Breast cancer; HOPE; Microarray; Northern blot; RNA

Indexed keywords

ACETONE; BUFFER; DNA; FORMALDEHYDE; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HIGH MOBILITY GROUP B1 PROTEIN; PARAFFIN; RNA;

ARTICLE; BREAST CANCER; CANCER GRADING; CONTROLLED STUDY; DNA MICROARRAY; HISTOLOGY; HUMAN; HUMAN TISSUE; HYBRIDIZATION; MOLECULAR BIOLOGY; NORTHERN BLOTTING; QUALITATIVE ANALYSIS;

EID: 10644219520     PISSN: 03440338     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.prp.2004.04.008     Document Type: Article

References (14)

1. A. Bosio, C. Knorr, U. Janssen, S. Gebel, H.J. Haussmann, T. Muller, Kinetics of gene expression profiling in Swiss 3T3 cells exposed to aqueous extracts of cigarette smoke, Carcinogenesis 23 (2002) 741-748.
2. P.A. Clarke, R. te Poele, R. Wooster, P. Workman, Gene expression microarray analysis in cancer biology, pharmacology, and drug development: progress and potential, Biochem. Pharmacol. 62 (2001) 1311-1336.
3. D. Droemann, T. Goldmann, D. Branscheid, R. Clark, K. Dalhoff, P. Zabel, E. Vollmer, Toll-like receptor 2 is expressed by alveolar epithelial cells type II and macrophages in the human lung, Histochem. Cell Biol. 119 (2003) 103-108.
4. J. Eberwine, Amplification of mRNA populations using aRNA generated from immobilized oligo(dT)-T7 primed cDNA, Biotechniques 20 (1996) 584-591.
5. A.P. Feinberg, B. Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity, Anal. Biochem. 132 (1983) 6-13.
6. A.M. Flohr, P. Rogalla, M. Meiboom, L. Borrmann, M. Krohn, B. Thode-Halle, J. Bullerdiek, Variation of HMGB1 expression in breast cancer, Anticancer Res. 21 (2001) 3881-3885.
7. T. Goldmann, K.H. Wiedorn, H. Kuhl, J. Olert, D. Branscheid, D. Pechkovsky, G. Zissel, J. Galle, J. Muller-Quernheim, E. Vollmer, Assessment of transcriptional gene activity in situ by application of HOPE-fixed, paraffin-embedded tissues, Pathol. Res. Pract. 198 (2002) 91-95.
8. T. Goldmann, E. Vollmer, J. Gerdes, What's cooking? Detection of important biomarkers in HOPE-fixed paraffin embedded tissues eliminates the need for antigen retrieval, Am. J. Pathol. 163 (2003) 2638-2640.
9. F. Lewis, N.J. Maughan, V. Smith, K. Hillan, P. Quirke, Unlocking the archive-gene expression in paraffin-embedded tissue, J. Pathol. 195 (2001) 66-71.
10. J. Olert, K.H. Wiedorn, T. Goldmann, H. Kühl, Y. Mehraein, H. Scherthan, F. Niketeghad, E. Vollmer, A.M. Müller, J. Müller-Navia, HOPE-fixation: a novel fixing method and paraffin embedding technique for human soft tissues, Pathol. Res. Pract. 197 (2001) 823-826.
11. S. Tomiuk, K. Hofmann, Microarray probe selection strategies, Brief Bioinform. 2 (2001) 329-340.
12. O. Umland, A.J. Ulmer, E. Vollmer, T. Goldmann, HOPE fixation of cytospin preparations of human cells for in situ hybridization and immunocytochemistry, J. Histochem. Cytochem. 51 (2003) 977-980.
13. L. Wen, J.K. Huang, B.H. Johnson, G.R. Reeck, A human placental cDNA clone that encodes nonhistone chromosomal protein HMG-1, Nucl. Acids Res. 17 (1989) 1197-1214.
14. K.H. Wiedorn, J. Olert, R.A. Stacy, T. Goldmann, H. Kuhl, J. Matthus, E. Vollmer, A. Bosse, HOPE - a new fixing technique enables preservation and extraction of high molecular weight DNA and RNA of >20kb from paraffin-embedded tissues. Hepes-Glutamic acid buffer mediated Organic solvent Protection Effect, Pathol. Res. Pract. 198 (2002) 735-740.