HOPE fixation of cytospin preparations of human cells for in situ hybridization and immunocytochemistry

Journal of Histochemistry and Cytochemistry

Volumn 51, Issue 7, 2003, Pages 977-980

  Umland, Oliver ^a   Ulmer, Artur J ^a   Vollmer, Ekkehard ^a   Goldmann, Torsten ^a  

^a RESEARCH CENTER BORSTEL   (Germany)

Author keywords

Carnoy's solution; Cultured cells; Cytokines; Cytospins; HOPE fixation; ICC; ISH; MRNA; Paraformaldehyde

Indexed keywords

4 (2 HYDROXYETHYL) 1 PIPERAZINEETHANESULFONIC ACID; CYTOKINE; CYTOSPIN; GLUTAMIC ACID; MESSENGER RNA; ORGANIC SOLVENT; UNCLASSIFIED DRUG;

ARTICLE; CELL CULTURE; GENE EXPRESSION; HEMATOPOIETIC STEM CELL; HOPE FIXATION; HUMAN; HUMAN CELL; IMMUNOCYTOCHEMISTRY; IMMUNOHISTOCHEMISTRY; IN SITU HYBRIDIZATION; LUNG PARENCHYMA; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; TISSUE FIXATION;

EID: 0038648672     PISSN: 00221554     EISSN: None     Source Type: Journal    
DOI: 10.1177/002215540305100714     Document Type: Article

References (9)

1. Goldmann T, Suter L, Ribbert D, Otto F (1999) The expression of proteolytic enzymes at the dermal invading front of primary cutaneous melanoma predicts metastasis. Pathol Res Pract 195:171-175
2. Goldmann T, Wiedorn KH, Kuhl H, Olert J, Branscheid D, Pechkovsky D, Zissel G, et al. (2002) Assessment of transcriptional gene activity in situ by application of HOPE-fixed, paraffin-embedded tissues. Pathol Res Pract 198:91-95
3. Kanbe N, Kurosawa M, Miyachi Y, Kanbe M, Kempuraj D, Tachimoto H, Saito H (1998) Carnoy's fixative reduces the number of chymase-positive cells in immunocytochemical staining of cordblood-derived human cultured mast cells. Allergy 53:981-985
4. Kovacs W, Stangl H, Volkl A, Schad A, Dariush FH, Baumgart E (2001) Localization of mRNAs encoding peroxisomal proteins in cell culture by non-radioactive in situ hybridization. Comparison of rat and human hepatoma cells and their responses to two divergent hypolipidemic drugs. Histochem Cell Biol 115:499-508
5. Olert J, Wiedorn KH, Goldmann T, Kuhl H, Mehraein Y, Scherthan H, Niketeghad F, et al. (2001) HOPE fixation: a novel fixing method and paraffin-embedding technique for human soft tissues. Pathol Res Pract 197:823-826
6. Pechkovsky DV, Zissel G, Goldmann T, Einhaus M, Taube C, Magnussen H, Schlaak M, et al. (2002a) Pattern of NOS2 and NOS3 mRNA expression in human A549 cells and primary cultured AEC II. Am J Physiol 282:L684-692
7. Pechkovsky DV, Zissel G, Stamme C, Goldmann T, Ari JH, Einhaus M, Taube C, et al. (2002b) Human alveolar epithelial cells induce nitric oxide synthase-2 expression in alveolar macrophages. Eur Respir J 19:672-683
8. Roche Molecular Biochemicals (1996) Nonradioactive In Situ Hybridization Application Manual. 2nd ed. Mannheim, Germany, Roche Diagnostics, Boehringer Mannheim GmbH
9. Uhlig U, Haitsma JJ, Goldmann T, Poelma DL, Lachmann B, Uhlig S (2002) Ventilation-induced activation of the mitogen activated proteinkinase pathway. Eur Resp J 20:946-956