Characterisation of CD154+ T cells following ex vivo birch allergen stimulation defines a close relationship between T cell subsets in healthy volunteers

BMC Immunology

Volumn 14, Issue 1, 2013, Pages

  Smith, Karen A ^a   Gray, Nicola J ^b   Cheek, Elizabeth ^c   Saleh, Femi ^b   Lavender, Jo ^b   Frew, Anthony J ^b   Kern, Florian ^a   Tarzi, Michael D ^a  

^a UNIVERSITY OF SUSSEX   (United Kingdom)

^b ROYAL SUSSEX COUNTY HOSPITAL   (United Kingdom)

^c UNIVERSITY OF BRIGHTON   (United Kingdom)

Author keywords

Allergen specific T cell; Birch pollen allergy; Ex vivo phenotyping; Flow cytometry

Indexed keywords

ALLERGEN; CD27 ANTIGEN; CD40 LIGAND; CD45RA ANTIGEN; GAMMA INTERFERON; IMMUNOGLOBULIN E; IMMUNOGLOBULIN G4; INTERLEUKIN 10; INTERLEUKIN 4;

ADULT; ARTICLE; BIRCH; BLOOD SAMPLING; CLINICAL ARTICLE; CONTROLLED STUDY; EX VIVO STUDY; FEMALE; HUMAN; HUMAN CELL; MALE; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; POLLEN ALLERGY; PROTEIN EXPRESSION; REGULATORY T LYMPHOCYTE; T LYMPHOCYTE SUBPOPULATION; TH1 CELL; TH2 CELL;

ADULT; ALLERGENS; ANTIGENS, CD27; ANTIGENS, CD45; BETULA; CD40 LIGAND; CELL DIFFERENTIATION; CYTOKINES; EPITOPES; FEMALE; HEALTH; HUMANS; IMMUNE TOLERANCE; LYMPHOCYTE ACTIVATION; MALE; PHENOTYPE; POLLEN; RHINITIS, ALLERGIC, SEASONAL; SEASONS; T-LYMPHOCYTE SUBSETS; T-LYMPHOCYTES; T-LYMPHOCYTES, REGULATORY; TH1 CELLS; TH2 CELLS;

EID: 84875200519     PISSN: None     EISSN: 14712172     Source Type: Journal    
DOI: 10.1186/1471-2172-14-14     Document Type: Article

References (28)

1. Wambre E, Delong J, James E, LaFond R, Robinson D, Kwok W. Differentiation stage determines pathological and protective allergen-specific CD4+ T cell outcomes during specific immunotherapy. J Allergy Clin Immunol 2011, 129(2):544-551. 551.e1-7, 3268867, 21975176.
2. Akdis M, Verhagen J, Taylor A, Karamloo F, Karagiannidis C, Crameri R. Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J Exp Med 2004, 199(11):1567-1575. 10.1084/jem.20032058, 2211782, 15173208.
3. Thomas WR. CD4 allergen tetramers. Clin Exp Allergy 2011, 41(6):769-772. 10.1111/j.1365-2222.2011.03751.x, 21561492.
4. Chattopadhyay PK, Yu J, Roederer M. Live-cell assay to detect antigen-specific CD4(+) T-cell responses by CD154 expression. Nat Protoc 2006, 1(1):1-6.
5. Frentsch M, Arbach O, Kirchhoff D, Moewes B, Worm M, Rothe M. Direct access to CD4(+) T cells specific for defined antigens according to CD154 expression. Nat Med 2005, 11(10):1118-1124. 10.1038/nm1292, 16186818.
6. Van Hemelen D, Oude Elberink J, Bohle B, Heimweg J, Nawijn M, Van Oosterhout A. Flow cytometric analysis of cytokine expression in short-term allergen-stimulated T cells mirrors the phenotype of proliferating T cells in long-term cultures. J Immunol Methods 2011, 371:114-121. 10.1016/j.jim.2011.06.019, 21741978.
7. Campbell J, Buchmann P, Cunningham C, Kesting S, Coffman R, Hessel E. Allergen-specific T cell responses to immunotherapy monitored by CD154 and intracellular cytokine expression. Clin Exp Allergy 2010, 40(7):1025-1035. 10.1111/j.1365-2222.2010.03505.x, 20412135.
8. Wambre E, Bonvalet M, Bodo V, Maillere B, Leclert G, Moussu H. Distinct characteristics of seasonal (Bet v 1) vs. perennial (Der p 1) allergen-specific CD4+ T cell responses. Clin Exp Allergy 2010, 41:192-203.
9. Bateman E, Ardern-Jones M, Ogg G. Persistent central memory phenotype of circulating Fel d 1 peptide/DRB1*0101 tetramer-binding CD4+ T cells. J Allergy Clin Immunol 2006, 118(6):1350-1356. 10.1016/j.jaci.2006.07.040, 17137868.
10. Bonvalet M, Wambre E, Moussu H, Horiot S, Kwok W, Louise A. Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen-specific CD4(+) T cells. Clin Exp Allergy 2011, 41(6):821-829. 10.1111/j.1365-2222.2011.03708.x, 21418343.
11. Crack L, Chan H, McPherson T, Ogg G. Phenotypic analysis of perennial airborne allergen-specific CD4+ T cells in atopic and non-atopic individuals. Clin Exp Allergy 2011, 41:1555-1567. 10.1111/j.1365-2222.2011.03819.x, 21729183.
12. Platts-Mills T, Vaughan J, Squillace S, Woodfolk J, Sporik R. Sensitisation, asthma, and a modified Th2 response in children exposed to cat allergen: a population-based cross-sectional study. Lancet 2001, 357:752-756. 10.1016/S0140-6736(00)04168-4, 11253969.
13. Reefer AJ, Carneiro RM, Custis NJ, Platts-Mills TA, Sung SS, Hammer J. A role for IL10-mediated HLA-DR7-restricted T cell-dependent events in development of the modified Th2 response to cat allergen. J Immunol 2004, 172:2763-2772.
14. Meiler F, Zumkehr J, Klunker S, Ruckert B, Akdis C, Akdis M. In vivo switch to IL10-secreting T regulatory cells in high dose allergen exposure. J Exp Med 2008, 205(12):2887-2898. 10.1084/jem.20080193, 2585856, 19001136.
15. Francis JN, Till SJ, Durham SR. Induction of IL-10 + CD4 + CD25+ T cells by grass pollen immunotherapy. J Allergy Clin Immunol 2003, 111(6):1255-1261. 10.1067/mai.2003.1570, 12789226.
16. Jutel M, Akdis M, Budak F, Aebischer-Casaulta C, Wrzyszcz M, Blaser K. IL-10 and TGF-beta cooperate in the regulatory T cell response to mucosal allergens in normal immunity and specific immunotherapy. Eur J Immunol 2003, 33(5):1205-1214. 10.1002/eji.200322919, 12731045.
17. Campbell J, Buckland K, McMillan S, Kearley J, Oldfield W, Stern L. Peptide immunotherapy in allergic asthma generates IL10-dependent immunological tolerance associated with linked epitope suppression. J Exp Med 2009, 206(7):1535-1547. 10.1084/jem.20082901, 2715096, 19528258.
18. Roncarolo MG, Gregori S. The validity of Foxp3 to define human and mouse regulatory T cells: is Foxp3 a bona fide marker for human regulatory T cells?. Eur J Immunol 2008, 38:901-937. 10.1002/eji.200890012, 18395855.
19. Levings MK, Roncarolo MG. Phenotypic and functional differences between human CD4 + CD25+ and type 1 regulatory T cells. Curr Top Microbiol Immunol 2005, 293:303-326. 10.1007/3-540-27702-1_14, 15981486.
20. Mobs C, Slotosch C, Loffler H, Jakob T, Hertl M, Pfutzner W. Birch pollen immunotherapy leads to differential induction of regulatory T cells and delayed helper T cell immune deviation. J Immunol 2010, 184:2194-2203. 10.4049/jimmunol.0901379, 20048125.
21. Hawrylowicz C, O'Garra A. Potential role of interleukin-10-secreting regulatory T cells in allergy and asthma. Nat Rev Immunol 2005, 5(4):271-283. 10.1038/nri1589, 15775993.
22. Arif S, Tree T, Astill T, Tremble J, Bishop A, Dayan C. Autoreactive T cell responses show proinflammatory polarisation in diabetes but a regulatory phenotype in health. J Clin Invest 2004, 113(3):451-463. 324541, 14755342.
23. Tree T, Lawson J, Edwards H, Skowera A, Arif S, Roep B. Naturally arising human CD4 T cells that recognise islet autoantigens and secrete interleukin-10 regulate proinflammatory T cell responses via linked suppression. Diabetes 2010, 59(6):1451-1460. 10.2337/db09-0503, 2874706, 20299476.
24. Satoguina J, Weyand E, Larbi J, Hoerauf A. T regulatory 1 cells induce IgG4 production by B cells: Role of IL10. J Immunol 2005, 174:4718-4726.
25. Shamji M, Durham S. Mechanisms of immunotherapy to aeroallergens. Clin Exp Allergy 2011, 41(9):1235-1246. 10.1111/j.1365-2222.2011.03804.x, 21762223.
26. Van Overtwelt L, Wambre E, Maillere B, von Hofe E, Louise A, Balazuc A. Assessment of Bet v 1-specific CD4+ T cell responses in allergic and nonallergic individuals using MHC Class II peptide tetramers. J Immunol 2008, 180:4514-4522.
27. Berard M, Tough D. Qualitative differences between naive and memory T cells. Immunology 2002, 106:127-138. 10.1046/j.1365-2567.2002.01447.x, 1782715, 12047742.
28. Geginat J, Lanzavecchia A, Sallusto F. Proliferation and differentiation potential of human CD8+ memory T cell-subsets in response to antigen or homeostatic cytokines. Blood 2003, 101:4260-4266. 10.1182/blood-2002-11-3577, 12576317.