Flow cytometric analysis of cytokine expression in short-term allergen-stimulated T cells mirrors the phenotype of proliferating T cells in long-term cultures

Journal of Immunological Methods

Volumn 371, Issue 1-2, 2011, Pages 114-121

  Van Hemelen, D ^a   Oude Elberink, J N G ^a   Bohle, B ^b   Heimweg, J ^a   Nawijn, M C ^a   Van Oosterhout, A J M ^a  

^a UNIVERSITY MEDICAL CENTER GRONINGEN   (Netherlands)

^b MEDICAL UNIVERSITY OF VIENNA   (Austria)

Author keywords

Antigen specific TH cells; CD154; CFSE; Flow cytometry; Grass pollen allergy

Indexed keywords

ALLERGEN; CARBOXYFLUORESCEIN; CD40 LIGAND; CYTOKINE; GAMMA INTERFERON; GRASS POLLEN EXTRACT; INTERLEUKIN 4;

ADULT; ANTIGEN EXPRESSION; ARTICLE; CELL CLONE; CELL CULTURE; CELL LINE; CELL PROLIFERATION; CLINICAL ARTICLE; CONTROLLED STUDY; EX VIVO STUDY; FLOW CYTOMETRY; GRASS POLLEN; HELPER CELL; HUMAN; HUMAN CELL; MALE; PERIPHERAL BLOOD MONONUCLEAR CELL; PHENOTYPE; PHENOTYPIC VARIATION; PRIORITY JOURNAL; PROTEIN EXPRESSION; T LYMPHOCYTE;

ADULT; ALLERGENS; CD40 LIGAND; CELL CULTURE TECHNIQUES; CELL PROLIFERATION; CYTOKINES; FEMALE; FLOW CYTOMETRY; HUMANS; IMMUNOASSAY; IMMUNOPHENOTYPING; MALE; PHENOTYPE; POLLEN; RHINITIS, ALLERGIC, SEASONAL; T-LYMPHOCYTES, HELPER-INDUCER;

EID: 79960958674     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2011.06.019     Document Type: Article

References (27)

1. Akdis M., Trautmann A., Klunker S., Daigle I., Kucuksezer U.C., Deglmann W., Disch R., Blaser K., Akdis C.A. T helper (Th) 2 predominance in atopic diseases is due to preferential apoptosis of circulating memory/effector Th1 cells. FASEB J. 2003, 17:1026.
2. Akdis M., Verhagen J., Taylor A., Karamloo F., Karagiannidis C., Crameri R., Thunberg S., Deniz G., Valenta R., Fiebig H., Kegel C., Disch R., Schmidt-Weber C.B., Blaser K., Akdis C.A. Immune responses in healthy and allergic individuals are characterized by a fine balance between allergen-specific T regulatory 1 and T helper 2 cells. J. Exp. Med. 2004, 199:1567.
3. Bird J.J., Brown D.R., Mullen A.C., Moskowitz N.H., Mahowald M.A., Sider J.R., Gajewski T.F., Wang C.R., Reiner S.L. Helper T cell differentiation is controlled by the cell cycle. Immunity 1998, 9:229.
4. Bohle B., Zwolfer B., Fischer G.F., Seppala U., Kinaciyan T., Bolwig C., Spangfort M.D., Ebner C. Characterization of the human T cell response to antigen 5 from Vespula vulgaris (Ves v 5). Clin. Exp. Allergy 2005, 35:367.
5. Bonvalet M., Wambre E., Moussu H., Horiot S., Kwok W.W., Louise A., Ebo D., Hoarau C., Van Overtvelt L., Baron-Bodo V., Moingeon P. Comparison between major histocompatibility complex class II tetramer staining and surface expression of activation markers for the detection of allergen-specific CD4(+) T cells. Clin. Exp. Allergy 2011, 41:821.
6. Bullens D.M., Van Den Keybus C., Dilissen E., Kasran A., Ceuppens J.L. Allergen-specific T cells from birch-pollen-allergic patients and healthy controls differ in T helper 2 cytokine and in interleukin-10 production. Clin. Exp. Allergy 2004, 34:879.
7. Campbell J.D., Buchmann P., Kesting S., Cunningham C.R., Coffman R.L., Hessel E.M. Allergen-specific T cell responses to immunotherapy monitored by CD154 and intracellular cytokine expression. Clin Exp Allergy 2010, 40:1025.
8. Casares S., Bona C.A., Brumeanu T.D. Enzymatically mediated engineering of multivalent MHC class II-peptide chimeras. Protein Eng. 2001, 14:195.
9. de St Groth Fazekas, Smith A.L., Koh W.P., Girgis L., Cook M.C., Bertolino P. Carboxyfluorescein diacetate succinimidyl ester and the virgin lymphocyte: a marriage made in heaven. Immunol. Cell Biol. 1999, 77:530.
10. de Waal Malefyt R., Yssel H., de Vries J.E. Direct effects of IL-10 on subsets of human CD4+ T cell clones and resting T cells. Specific inhibition of IL-2 production and proliferation. J. Immunol. 1993, 150:4754.
11. Ebner C., Schenk S., Najafian N., Siemann U., Steiner R., Fischer G.W., Hoffmann K., Szepfalusi Z., Scheiner O., Kraft D. Nonallergic individuals recognize the same T cell epitopes of Bet v 1, the major birch pollen allergen, as atopic patients. J. Immunol. 1995, 154:1932.
12. Frentsch M., Arbach O., Kirchhoff D., Moewes B., Worm M., Rothe M., Scheffold A., Thiel A. Direct access to CD4+ T cells specific for defined antigens according to CD154 expression. Nat. Med. 2005, 11:1118.
13. Gett A.V., Hodgkin P.D. Cell division regulates the T cell cytokine repertoire, revealing a mechanism underlying immune class regulation. Proc. Natl. Acad. Sci. U. S. A. 1998, 95:9488.
14. Givan A.L., Fisher J.L., Waugh M., Ernstoff M.S., Wallace P.K. A flow cytometric method to estimate the precursor frequencies of cells proliferating in response to specific antigens. J. Immunol. Methods 1999, 230:99.
15. Hackett C.J., Sharma O.K. Frontiers in peptide-MHC class II multimer technology. Nat. Immunol. 2002, 3:887.
16. Houck D.W., Loken M.R. Simultaneous analysis of cell surface antigens, bromodeoxyuridine incorporation and DNA content. Cytometry 1985, 6:531.
17. Lindstedt M., Schiott A., Bengtsson A., Larsson K., Korsgren M., Greiff L., Borrebaeck C.A. Genomic and functional delineation of dendritic cells and memory T cells derived from grass pollen-allergic patients and healthy individuals. Int. Immunol. 2005, 17:401.
18. Magnan A.O., Mely L.G., Camilla C.A., Badier M.M., Montero-Julian F.A., Guillot C.M., Casano B.B., Prato S.J., Fert V., Bongrand P., Vervloet D. Assessment of the Th1/Th2 paradigm in whole blood in atopy and asthma. Increased IFN-gamma-producing CD8(+) T cells in asthma. Am. J. Respir. Crit. Care Med. 2000, 161:1790.
19. Moverare R., Elfman L., Stalenheim G., Bjornsson E. Study of the Th1/Th2 balance, including IL-10 production, in cultures of peripheral blood mononuclear cells from birch-pollen-allergic patients. Allergy 2000, 55:171.
20. Munier C.M., Zaunders J.J., Ip S., Cooper D.A., Kelleher A.D. A culture amplified multi-parametric intracellular cytokine assay (CAMP-ICC) for enhanced detection of antigen specific T-cell responses. J. Immunol. Methods 2009, 345:1.
21. Rimaniol A.C., Garcia G., Till S.J., Capel F., Gras G., Balabanian K., Emilie D., Humbert M. Evaluation of CD4+ T cells proliferating to grass pollen in seasonal allergic subjects by flow cytometry. Clin. Exp. Immunol. 2003, 132:76.
22. Romagnani S. The role of lymphocytes in allergic disease. J. Allergy Clin. Immunol. 2000, 105:399.
23. Thiel A., Scheffold A., Radbruch A. Antigen-specific cytometry-new tools arrived!. Clin. Immunol. 2004, 111:155.
24. Van Neerven R.J., Van de Pol M.M., Van der Zee J.S., Stiekema F.E., De Boer M., Kapsenberg M.L. Requirement of CD28-CD86 costimulation for allergen-specific T cell proliferation and cytokine expression. Clin. Exp. Allergy 1998, 28:808.
25. Van Overtvelt L., Wambre E., Maillere B., von Hofe E., Louise A., Balazuc A.M., Bohle B., Ebo D., Leboulaire C., Garcia G., Moingeon P. Assessment of Bet v 1-specific CD4+ T cell responses in allergic and nonallergic individuals using MHC class II peptide tetramers. J. Immunol. 2008, 180:4514.
26. Wambre E., Van Overtvelt L., Maillere B., Humphreys R., von Hofe E., Ferhat L., Ebo D., Moingeon P. Single cell assessment of allergen-specific T cell responses with MHC class II peptide tetramers: methodological aspects. Int. Arch. Allergy Immunol. 2008, 146:99.
27. Wierenga E.A., Snoek M., Jansen H.M., Bos J.D., van Lier R.A., Kapsenberg M.L. Human atopen-specific types 1 and 2 T helper cell clones. J. Immunol. 1991, 147:2942.