Enhancement of the efficiency of secretion of heterologous lipase in Escherichia coli by directed evolution of the ABC transporter system

Applied and Environmental Microbiology

Volumn 71, Issue 7, 2005, Pages 3468-3474

  Eom, Gyeong Tae ^a   Song, Jae Kwang ^b   Ahn, Jung Hoon ^a   Seo, Yeon Soo ^a   Rhee, Joon Shick ^a  

^a Korea Advanced Institute of Science and Technology (KAIST)   (South Korea)

^b Korea Research Institute of Chemical Technology   (South Korea)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; BIOASSAY; CELL MEMBRANES; ESCHERICHIA COLI; MUTAGENESIS;

LIPASE; MICROTITER-PLATE BASED ASSAYS; MUTANTS; TRIBUTYRIN;

ENZYMES;

ABC TRANSPORTER; BACTERIAL PROTEIN; DNA FRAGMENT; MUTANT PROTEIN; PROTEINASE; RECOMBINANT PROTEIN; TRIACYLGLYCEROL LIPASE;

ENZYME ACTIVITY; METABOLISM;

ARTICLE; BACTERIUM CULTURE; ENZYME ASSAY; ENZYME RELEASE; ESCHERICHIA COLI; GENE; IMMUNOBLOTTING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PSEUDOMONAS FLUORESCENS; TLIA GENE; TLID GENE;

ATP-BINDING CASSETTE TRANSPORTERS; BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ESCHERICHIA COLI; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; HEAT; LIPASE; MUTATION; PSEUDOMONAS FLUORESCENS; RECOMBINANT PROTEINS;

ESCHERICHIA COLI; PSEUDOMONAS FLUORESCENS;

EID: 22144461805     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.71.7.3468-3474.2005     Document Type: Article

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