Searching for microbial protein over-expression in a complex matrix using automated high throughput MS-based proteomics tools

Journal of Biotechnology

Volumn 164, Issue 1, 2013, Pages 112-120

  Akeroyd, Michiel ^a   Olsthoorn, Maurien ^a   Gerritsma, Jort ^a   Gutker Vermaas, Diana ^a   Ekkelkamp, Laurens ^a   van Rij, Tjeerd ^a   Klaassen, Paul ^a   Plugge, Wim ^a   Smit, Ed ^a   Strupat, Kerstin ^b   Wenzel, Thibaut ^a   van Tilborg, Marcel ^a   van der Hoeven, Rob ^a  

^a DSM BIOTECHNOLOGY CENTER   (Netherlands)

^b THERMO FISHER SCIENTIFIC   (Germany)

Author keywords

Expression libraries; High throughput; MALDI Orbitrap; MS screening; Protein over expression; UHPLC

Indexed keywords

HIGH THROUGHPUT; HIGH THROUGHPUT SCREENING; MALDI-ORBITRAP; OVER-EXPRESSION; PEPTIDE MASS FINGERPRINTING; PROTEIN IDENTIFICATION; PROTEIN PRECIPITATION; UHPLC;

BIODIVERSITY; CLONING; ENZYMES; LIBRARIES; MASS SPECTROMETRY; MOLECULAR BIOLOGY; PRINCIPAL COMPONENT ANALYSIS; SCREENING; THROUGHPUT;

PROTEINS;

BOVINE SERUM ALBUMIN; MICROORGANISM PROTEIN; TRYPSIN;

ARTICLE; FINGER DERMATOGLYPHICS; HIGH THROUGHPUT SCREENING; MASS SPECTROMETRY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MICROTITER PLATE ASSAY; MOLECULAR WEIGHT; NONHUMAN; PRECIPITATION; PRINCIPAL COMPONENT ANALYSIS; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEOMICS; TANDEM MASS SPECTROMETRY; ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY;

ASPERGILLUS NIGER; BACILLUS SUBTILIS; BACTERIAL PROTEINS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; DATABASES, PROTEIN; FERMENTATION; FUNGAL PROTEINS; HIGH-THROUGHPUT SCREENING ASSAYS; PEPTIDE MAPPING; PROTEOMICS; SERUM ALBUMIN, BOVINE; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; TANDEM MASS SPECTROMETRY; TRICHLOROACETIC ACID; TRYPSIN;

BACTERIA (MICROORGANISMS); BOVINAE; FUNGI;

EID: 84875084784     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2012.11.015     Document Type: Article

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