Changing the substrate specificity of P450cam towards diphenylmethane by semi-rational enzyme engineering

Protein Engineering, Design and Selection

Volumn 24, Issue 5, 2011, Pages 439-446

  Hoffmann, Gregor ^a   Bönsch, Kathrin ^a   Greiner Stöffele, Thomas ^a,b   Ballschmiter, Meike ^a  

^a UNIVERSITY OF LEIPZIG   (Germany)

^b c LEcta GmbH   (Germany)

Author keywords

cluster screening; diphenylmethane; focused library; P450cam; protein engineering

Indexed keywords

CLUSTER SCREENING; DIPHENYLMETHANE; FOCUSED LIBRARY; P450CAM; PROTEIN ENGINEERING;

AMINO ACIDS; BIOCHEMICAL ENGINEERING; GENETIC ENGINEERING; MUTAGENESIS;

PROTEINS;

4 BENZYLPHENOL; BACTERIAL ENZYME; CAMPHOR 5 MONOOXYGENASE; CAMPHOR DERIVATIVE; DEXTRO CAMPHOR; DIPHENYLMETHANE; ENZYME; MUTANT PROTEIN; OXIDOREDUCTASE; PEPTIDE LIBRARY; POLYHISTIDINE TAG; PUTIDAREDOXIN; PUTIDAREDOXIN REDUCTASE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CRYSTAL STRUCTURE; DEPLETION; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME SPECIFICITY; ESCHERICHIA COLI; HYDROXYLATION; IN VITRO STUDY; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN LOCALIZATION; PROTEIN PURIFICATION; PSEUDOMONAS PUTIDA; SITE DIRECTED MUTAGENESIS; WILD TYPE;

BENZHYDRYL COMPOUNDS; CAMPHOR 5-MONOOXYGENASE; CATALYTIC DOMAIN; DRUG DISCOVERY; HYDROXYLATION; MODELS, MOLECULAR; MUTAGENESIS; MUTATION; PROTEIN ENGINEERING; PSEUDOMONAS PUTIDA; STEREOISOMERISM; SUBSTRATE SPECIFICITY;

DRYOBALANOPS;

EID: 79955432494     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzq119     Document Type: Article

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