High yield production of a soluble bifidobacterial β-galactosidase (BbgIV) in E. Coli DH5α with improved catalytic efficiency for the synthesis of prebiotic galactooligosaccharides

Journal of Agricultural and Food Chemistry

Volumn 61, Issue 9, 2013, Pages 2213-2223

  Osman, Ali ^a   Tzortzis, George ^b   Rastall, Robert A ^a   Charalampopoulos, Dimitris ^a  

^a UNIVERSITY OF READING   (United Kingdom)

^b Clasado Ltd   (United Kingdom)

Author keywords

galactosidase; Bifidobacterium; dissolved oxygen; prebiotic galactooligosaccharides; protein expression; specific activity

Indexed keywords

BIFIDOBACTERIUM; GALACTO-OLIGOSACCHARIDES; GALACTOSIDASES; PROTEIN EXPRESSIONS; SPECIFIC ACTIVITY;

DISSOLVED OXYGEN; ENZYMES; ESCHERICHIA COLI; PROTEINS;

CATALYST ACTIVITY;

BETA GALACTOSIDASE; GALACTOSE; LACTOSE; OLIGOSACCHARIDE; PREBIOTIC AGENT; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM TRANSFORMATION; BIFIDOBACTERIUM; BIOCATALYSIS; BIOREACTOR; BIOSYNTHESIS; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; METABOLISM;

BETA-GALACTOSIDASE; BIFIDOBACTERIUM; BIOCATALYSIS; BIOREACTORS; ESCHERICHIA COLI; GALACTOSE; LACTOSE; OLIGOSACCHARIDES; PREBIOTICS; RECOMBINANT PROTEINS; TRANSFORMATION, BACTERIAL;

EID: 84874863505     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf304792g     Document Type: Article

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