Characterization of the metabolic burden on Escherichia coli DH1 cells imposed by the presence of a plasmid containing a gene therapy sequence

Biotechnology and Bioengineering

Volumn 88, Issue 7, 2004, Pages 909-915

  Rozkov, A ^a,c   Avignone Rossa, C A ^a   Ertl, P F ^b   Jones, P ^b   O'Kennedy, R D ^b   Smith, J J ^b   Dale, J W ^a   Bushell, Michael E ^a  

^a UNIVERSITY OF SURREY   (United Kingdom)

^b GLAXOSMITHKLINE   (United Kingdom)

^c ASTRAZENECA R AND D   (Sweden)

Author keywords

Escherichia coli; Metabolic burden; Plasmid

Indexed keywords

BIOMASS; BIOREACTORS; CELL CULTURE; CELLS; DNA; GENES; METABOLISM;

AERATION; AGITATION RATES; METABOLIC FLUX ANALYSIS (MFA); PLASMIDS;

ESCHERICHIA COLI;

CELL PROTEIN; HEPATITIS B ANTIGEN;

AERATION; AGITATION; ARTICLE; BACTERIAL CELL; BACTERIAL METABOLISM; BACTERIAL STRAIN; BIOLOGICAL MODEL; BIOREACTOR; CELL RESPIRATION; CITRIC ACID CYCLE; CONTROLLED STUDY; CYTOMEGALOVIRUS; ESCHERICHIA COLI; GENE SEQUENCE; GENE THERAPY; GENE THERAPY SEQUENCE; GROWTH RATE; IMMUNOTHERAPY; KAN GENE; MARKER GENE; METABOLIC FLUX ANALYSIS MODEL; MICROBIAL BIOMASS; NONHUMAN; PLASMID; PROMOTER REGION; SYNTHESIS;

BASE SEQUENCE; COMPUTER SIMULATION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENE THERAPY; HEPATITIS B ANTIGENS; KINETICS; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; PLASMIDS;

CUCUMBER MOSAIC VIRUS; ESCHERICHIA COLI;

EID: 10844293485     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/bit.20327     Document Type: Article

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