Convenient one-step purification and immobilization of lipase using a genetically encoded aldehyde tag

Biochemical Engineering Journal

Volumn 73, Issue , 2013, Pages 86-92

  Wang, Anming ^a,b   Du, Fangchuan ^a   Wang, Fen ^a   Shen, Yingqiang ^a   Gao, Weifang ^a   Zhang, Pengfei ^a  

^a HANGZHOU NORMAL UNIVERSITY   (China)

^b ZHEJIANG UNIVERSITY OF TECHNOLOGY   (China)

Author keywords

Aldehyde tag; Bioseparations; Enzyme activity; Immobilized enyzme; Lipase; Thermo stability

Indexed keywords

ACTIVATING AGENTS; ACTIVE SITE; ALDEHYDE-TAG; BIO SEPARATION; COVALENT IMMOBILIZATION; COVALENT LINKAGE; CROSSLINKER; ENZYME PURIFICATION; GLUTARALDEHYDES; HAZARDOUS CHEMICALS; IMMOBILIZATION METHOD; IMMOBILIZED ENYZME; IMMOBILIZED LIPASE; ONE-STEP PURIFICATION; ONE-STEP PURIFICATION AND IMMOBILIZATIONS; RECOMBINANT ENZYMES; RECOMBINANT LIPASE; SPECIFIC ACTIVITY; THERMO-STABILITY;

ALDEHYDES; ENZYME ACTIVITY; LIPASES; ORGANIC COMPOUNDS; PURIFICATION;

ENZYME IMMOBILIZATION;

ALDEHYDE TAGGED LIPASE; RECOMBINANT ENZYME; UNCLASSIFIED DRUG;

ARTICLE; BIOCATALYSIS; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME SYNTHESIS; GEOBACILLUS; NUCLEOTIDE SEQUENCE; PLASMID; PRIORITY JOURNAL; PROTEIN ENGINEERING; PROTEIN EXPRESSION; PROTEIN HYDROLYSIS; THERMOSTABILITY; TRANSGENICS;

EID: 84874791965     PISSN: 1369703X     EISSN: 1873295X     Source Type: Journal    
DOI: 10.1016/j.bej.2013.02.003     Document Type: Article

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