A novel and efficient method for the immobilization of thermolysin using sodium chloride salting-in and consecutive microwave irradiation

Bioresource Technology

Volumn 115, Issue , 2012, Pages 158-163

  Chen, Feifei ^a   Zhang, Fangkai ^a   Du, Fangchuan ^a   Wang, Anming ^a   Gao, Weifang ^a   Wang, Qiuyan ^a   Yin, Xiaopu ^a   Xie, Tian ^a  

^a HANGZHOU NORMAL UNIVERSITY   (China)

Author keywords

Immobilization; Microwave irradiation; Organic solvent; Salting in; Thermolysin

Indexed keywords

CONVENTIONAL TECHNIQUES; FREE ENZYME; IMMOBILIZATION TECHNIQUE; IMMOBILIZED ENZYME; MESOPOROUS SUPPORT; MICROWAVE POWER; MICROWAVE-ASSISTED; NO REDUCTION; SALTING-IN; SOLVENT DENATURATION; SPECIFIC ACTIVITY; THERMOLYSIN;

ENZYME ACTIVITY; MICROWAVE IRRADIATION; ORGANIC SOLVENTS; RADIOACTIVE WASTE VITRIFICATION; SODIUM CHLORIDE;

ENZYME IMMOBILIZATION;

CASEIN; MACROGOL; ORGANIC SOLVENT; SODIUM CHLORIDE; THERMOLYSIN;

CONCENTRATION (COMPOSITION); EFFICIENCY MEASUREMENT; ENZYME ACTIVITY; IMMOBILIZATION; IRRADIANCE; MICROWAVE RADIATION; SODIUM CHLORIDE; SOLVENT;

ARTICLE; BIOCATALYSIS; CHEMICAL ANALYSIS; CONCENTRATION (PARAMETERS); DENATURATION; DIFFUSION COEFFICIENT; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME MECHANISM; ENZYME SUBSTRATE; INCUBATION TEMPERATURE; LOW TEMPERATURE; MICHAELIS MENTEN KINETICS; MICROWAVE IRRADIATION; PEPTIDE SYNTHESIS; PRIORITY JOURNAL; PROTEIN HYDROLYSIS; THERMOSTABILITY;

ACETATES; BACILLUS; BIOTECHNOLOGY; DIPEPTIDES; ENZYME STABILITY; ENZYMES, IMMOBILIZED; HYDROLYSIS; KINETICS; MICROWAVES; PENTANOLS; SODIUM CHLORIDE; SOLVENTS; SUBSTRATE SPECIFICITY; TEMPERATURE; THERMOLYSIN;

EID: 84861456748     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2011.11.059     Document Type: Article

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