One step purification-immobilization of fuculose-1-phosphate aldolase, a class II DHAP dependent aldolase, by using metal-chelate supports

Enzyme and Microbial Technology

Volumn 39, Issue 1, 2006, Pages 22-27

  Ardao, Inés ^a   Benaiges, M Dolors ^a   Caminal, Gloria ^a   Álvaro, Gregorio ^a  

^a UNIVERSITAT AUTÒNOMA DE BARCELONA   (Spain)

Author keywords

DHAP dependent aldolase; Enzyme immobilization; Fuculose 1 phosphate aldolase (FucA); Immobilized metal chelate affinity chromatography (IMAC); Metal enzyme; One step purification immobilization of enzymes

Indexed keywords

AFFINITY CHROMATOGRAPHY; CHELATION; ESCHERICHIA COLI; METALS; PURIFICATION;

DHAP DEPENDENT ALDOLASE; FUCULOSE-1-PHOSPHATE ALDOLASE (FUCA); IMMOBILIZED METAL-CHELATE AFFINITY CHROMATOGRAPHY (IMAC); METAL-ENZYME; ONE STEP PURIFICATION-IMMOBILIZATION OF ENZYMES;

ENZYME IMMOBILIZATION;

ALANINE DERIVATIVE; BUFFER; CELL EXTRACT; COBALT; DIHYDROXYACETONE PHOSPHATE; FRUCTOSE BISPHOSPHATE ALDOLASE; FUCULOSEPHOSPHATE ALDOLASE; METAL CHELATE; UNCLASSIFIED DRUG;

AFFINITY CHROMATOGRAPHY; ALDOL REACTION; ARTICLE; CATALYSIS; CATALYST; CONTROLLED STUDY; DENSITY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STABILITY; ESCHERICHIA COLI; NONHUMAN; SOLUBILITY; TEMPERATURE MEASUREMENT;

ESCHERICHIA COLI;

EID: 33646039791     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2005.09.001     Document Type: Article

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