Abundance, distribution, mobility and oligomeric state of M2 muscarinic acetylcholine receptors in live cardiac muscle

Journal of Molecular and Cellular Cardiology

Volumn 57, Issue 1, 2013, Pages 129-136

  Nenasheva, Tatiana A ^a   Neary, Marianne ^a   Mashanov, Gregory I ^a   Birdsall, Nigel J M ^a   Breckenridge, Ross A ^a   Molloy, Justin E ^a  

^a NATIONAL INSTITUTE FOR MEDICAL RESEARCH   (United Kingdom)

Author keywords

Acetylcholine receptor; Cardiomyocyte; Muscarinic; Single molecule; TIRF

Indexed keywords

ACETYLCHOLINE; G PROTEIN COUPLED INWARDLY RECTIFYING POTASSIUM CHANNEL; MUSCARINIC M2 RECEPTOR;

ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ISOLATION; CHEMICAL LABELING; CONCENTRATION RESPONSE; CONFOCAL MICROSCOPY; CONTROLLED STUDY; EMBRYO; FEMALE; FLUORESCENCE MICROSCOPY; HEART MUSCLE; HEART MUSCLE CELL; LIGAND BINDING; MONTE CARLO METHOD; NONHUMAN; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; STOICHIOMETRY; TEMPERATURE DEPENDENCE;

ANIMALS; CARBOCYANINES; CHO CELLS; CRICETINAE; FLUORESCENT DYES; MICE; MICROSCOPY, FLUORESCENCE; MYOCARDIUM; MYOCYTES, CARDIAC; ORGAN SPECIFICITY; PRIMARY CELL CULTURE; PROTEIN STRUCTURE, QUATERNARY; PROTEIN TRANSPORT; RECEPTOR, MUSCARINIC M2; STAINING AND LABELING;

MURINAE; MUS;

EID: 84874183639     PISSN: 00222828     EISSN: 10958584     Source Type: Journal    
DOI: 10.1016/j.yjmcc.2013.01.009     Document Type: Article

References (36)

1. 2 muscarinic acetylcholine receptor bound to an antagonist. Nature 2012, 482:547-551.
2. Corey S., Krapivinsky G., Krapivinsky L., Clapham D.E. Number and stoichiometry of subunits in the native atrial G-protein-gated K(+) channel, I(KACh). J Biol Chem 1998, 273:5271-5278.
3. + channel is important for channel activation. Neuron 1995, 15:1133-1143.
4. + channels. Eur J Biochem 2000, 267:5830-5836.
5. Dhein S., Van Koppen C.J., Brodde O.E. Muscarinic receptors in the mammalian heart. Pharmacol Res 2001, 44:161-182.
6. Nishida M., MacKinnon R. Structural basis of inward rectification: cytoplasmic pore of the G protein-gated inward rectifier GIRK1 at 1.8 angstrom resolution. Cell 2002, 111:957-965.
7. Osterrieder W., Yang Q.F., Trautwein W. The time course of the muscarinic response to ionophoretic acetylcholine application to the S-A node of the rabbit heart. Pflugers Arch - Eur J Physiol 1981, 389:283-291.
8. Pott L., Pusch H. Kinetic-model for the muscarinic action of acetylcholine. Pflugers Arch - Eur J Physiol 1979, 383:75-77.
9. Krejci A., Tucek S. Quantitation of mRNAs for M-1 to M-5 subtypes of muscarinic receptors in rat heart and brain cortex. Mol Pharmacol 2002, 61:1267-1272.
10. 2 muscarinic receptors in rat ventricles with antipeptide antibodies. J Histochem Cytochem 1994, 42:337-343.
11. i protein-mediated muscarinic receptor adenylyl-cyclase system. Mol Cell Biochem 1995, 147:161-168.
12. Wang H.Z., Han H., Zhang L.M., Shi H., Schram G., Nattel S., et al. Expression of multiple subtypes of muscarinic receptors and cellular distribution in the human heart. Mol Pharmacol 2001, 59:1029-1036.
13. Jositsch G., Papadakis T., Haberberger R.V., Wolff M., Wess J., Kummer W. Suitability of muscarinic acetylcholine receptor antibodies for immunohistochemistry evaluated on tissue sections of receptor gene-deficient mice. Naunyn Schmiedebergs Arch Pharmacol 2009, 379:389-395.
14. Axelrod D. Total internal-reflection fluorescence microscopy. Methods Cell Biol 1989, 30:245-270.
15. 1 muscarinic receptor dimers seen by total internal reflection fluorescence imaging of single molecules. Proc Natl Acad Sci U S A 2010, 107:2693-2698.
16. Claycomb W.C., Lanson N.A., Stallworth B.S., Egeland D.B., Delcarpio J.B., Bahinski A., et al. HL-1 cells: a cardiac muscle cell line that contracts and retains phenotypic characteristics of the adult cardiomyocyte. Proc Natl Acad Sci U S A 1998, 95:2979-2984.
17. + current activated via muscarinic receptors comparable to that in mouse atrial myocytes. Pflugers Arch - Eur J Physiol 2010, 460:99-108.
18. 4 receptors: receptor subtype selectivity via cooperativity rather than affinity. Mol Pharmacol 2004, 65:257-266.
19. Mashanov G.I., Tacon D., Knight A.E., Peckham M., Molloy J.E. Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy. Methods 2003, 29:142-152.
20. Mashanov G.I., Molloy J.E. Automatic detection of single fluorophores in live cells. Biophys J 2007, 92:2199-2211.
21. Hulme E.C., Birdsall N.J.M., Buckley N.J. Muscarinic receptor subtypes. Annu Rev Pharmacol Toxicol 1990, 30:633-673.
22. Buckley N.J., Hulme E.C., Birdsall N.J.M. Use of clonal cell-lines in the analysis of neurotransmitter receptor mechanisms and function. Biochim Biophys Acta 1990, 1055:43-53.
23. 2 muscarinic acetylcholine receptor knockout mice. Proc Natl Acad Sci U S A 1999, 96:1692-1697.
24. 3 muscarinic receptor function and sensitivity to atrial arrhythmia in the RGS2-deficient mouse. Am J Physiol 2010, 298:H554-H561.
25. 4 receptor knockout mice: muscarinic receptor function in cardiac and smooth muscle in vitro. J Pharmacol Exp Ther 2000, 292:877-885.
26. Qian H., Sheetz M.P., Elson E.L. Single-particle tracking - analysis of diffusion and flow in 2-dimensional systems. Biophys J 1991, 60:910-921.
27. Ianoul A., Grant D.D., Rouleau Y., Bani-Yaghoub M., Johnston L.J., Pezacki J.P. Imaging nanometer domains of beta-adrenergic receptor complexes on the surface of cardiac myocytes. Nat Chem Biol 2005, 1:196-202.
28. Goin J.C., Nathanson N.M. Quantitative analysis of muscarinic acetylcholine receptor homo- and heterodimerization in live cells - regulation of receptor down-regulation by heterodimerization. J Biol Chem 2006, 281:5416-5425.
29. Ito Y., Kato A., Fujino T., Okura T., Yoshida K., Nanri M., et al. Muscarinic receptor binding and plasma drug concentration after the oral administration of propiverine in mice. Low Urin Tract Symptoms 2010, 2:43-49.
30. Harrell M.D., Harbi S., Hoffman J.F., Zavadil J., Coetzee W.A. Large-scale analysis of ion channel gene expression in the mouse heart during perinatal development. Physiol Genomics 2007, 28:273-283.
31. Trautwein W., Osterrieder W., Noma A. Potassium channels and the muscarinic receptor in the sino-atrial node of the heart. Drug receptors and their effectors 1981, 5-22. MacMillan. N.J.M. Birdsall (Ed.).
32. Hartzell H.C., Kuffler S.W., Stickgold R., Yoshikami D. Synaptic excitation and inhibition resulting from direct action of acetylcholine on 2 types of chemoreceptors on individual amphibian parasympathetic neurons. J Physiol 1977, 271:817-846.
33. Hillsmith I., Purves R.D. Synaptic delay in heart - ionophoretic study. J Physiol 1978, 279:31-54.
34. Nargeot J., Lester H.A., Birdsall N.J.M., Stockton J., Wassermann N.H., Erlanger B.F. A photoisomerizable muscarinic antagonist - studies of binding and of conductance relaxations in frog-heart. J Gen Physiol 1982, 79:657-678.
35. Raveh A., Riven I., Reuveny E. Elucidation of the gating of the GIRK channel using a spectroscopic approach. J Physiol (Lond) 2009, 587:5331-5335.
36. Riven I., Iwanir S., Reuveny E. GIRK channel activation involves a local rearrangement of a preformed G protein channel complex. Neuron 2006, 51:561-573.