Performance of clostridium difficile toxin enzyme immunoassay and nucleic acid amplification tests stratified by patient disease severity

Journal of Clinical Microbiology

Volumn 51, Issue 3, 2013, Pages 869-873

  Humphries, Romney M ^a   Uslan, Daniel Z ^a   Rubin, Zachary ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CLOSTRIDIUM DIFFICILE TOXIN A; CLOSTRIDIUM DIFFICILE TOXIN B; IMMUNOSUPPRESSIVE AGENT; NUCLEIC ACID;

ADULT; AGED; ARTICLE; BACTERIUM CULTURE; CLOSTRIDIUM DIFFICILE INFECTION; CONTROLLED STUDY; DIARRHEA; DISEASE SEVERITY; ENZYME IMMUNOASSAY; FEMALE; HOSPITAL PATIENT; HUMAN; LABORATORY TEST; MAJOR CLINICAL STUDY; MALE; MEDICAL RECORD; NONHUMAN; NUCLEIC ACID AMPLIFICATION; PRIORITY JOURNAL; PROSPECTIVE STUDY; RETROSPECTIVE STUDY; SENSITIVITY AND SPECIFICITY;

ADULT; AGED; AGED, 80 AND OVER; BACTERIAL TOXINS; BACTERIOLOGICAL TECHNIQUES; CLOSTRIDIUM DIFFICILE; CLOSTRIDIUM INFECTIONS; DIAGNOSTIC TESTS, ROUTINE; DIARRHEA; FEMALE; HUMANS; IMMUNOENZYME TECHNIQUES; MALE; MIDDLE AGED; NUCLEIC ACID AMPLIFICATION TECHNIQUES; PROSPECTIVE STUDIES; SENSITIVITY AND SPECIFICITY; SEVERITY OF ILLNESS INDEX; UNITED STATES;

EID: 84874041424     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02970-12     Document Type: Article

References (25)

1. Zilberberg M, Tillotson GS, Kollef M, Shorr A. 2011. Update on Clostridium difficile hospitalizations in the US through 2008: the epidemic continues, abstr 2169. CHEST 2011: Am. College Chest Physicians Annu. Meet., Honolulu, HI
2. Cohen SH, Gerding DN, Johnson S, Kelly CP, Loo VG, McDonald LC, Pepin J, Wilcox MH. 2010. Clinical practice guidelines for Clostridium difficile infection in adults: 2010 update by the Society for Healthcare Epidemiology of America (SHEA) and the Infectious Diseases Society of America (IDSA). Infect. Control Hosp. Epidemiol. 31:431-455
3. Boyanton BL, Jr, Sural P, Loomis CR, Pesta C, Gonzalez-Krellwitz L, Robinson-Dunn B, Riska P. 2012. Loop-mediated isothermal amplification compared to real-time PCR and enzyme immunoassay for toxigenic Clostridium difficile detection. J. Clin. Microbiol. 50:640-645
4. Doing KM, Hintz MS. 2012. Prospective evaluation of the Meridian Illumigene loop-mediated amplification assay and the Gen. Probe Pro-Gastro Cd polymerase chain reaction assay for the direct detection of toxigenic Clostridium difficile from fecal samples. Diagn. Microbiol. Infect. Dis. 72:8-13
5. Knetsch CW, Bakker D, de Boer RF, Sanders I, Hofs S, Kooistra-Smid AM, Corver J, Eastwood K, Wilcox MH, Kuijper EJ. 2011. Comparison of real-time PCR techniques to cytotoxigenic culture methods for diagnosing Clostridium difficile infection. J. Clin. Microbiol. 49:227-231
6. Lalande V, Barrault L, Wadel S, Eckert C, Petit JC, Barbut F. 2011. Evaluation of a loop-mediated isothermal amplification assay for diagnosis of Clostridium difficile infections. J. Clin. Microbiol. 49:2714-2716
7. Novak-Weekley SM, Marlowe EM, Miller JM, Cumpio J, Nomura JH, Vance PH, Weissfeld A. 2010. Clostridium difficile testing in the clinical laboratory by use of multiple testing algorithms. J. Clin. Microbiol. 48: 889-893
8. Planche TD, Davies KA, Coen PG, Crook D, Shetty N, Wren M, Wilcox MH. 2012. Clinical validation of Clostridium difficile infection (CDI) diagnostics importance of toxin detection, abstr D-160. Abstr. 52nd Intersci. Conf. Antimicrob. Agents Chemother., San Francisco, CA
9. Zar FA, Bakkanagari SR, Moorthi KM, Davis MB. 2007. A comparison of vancomycin and metronidazole for the treatment of Clostridium difficile-associated diarrhea, stratified by disease severity. Clin. Infect. Dis. 45:302-307
10. Curry SR, Marsh JW, Muto CA, O'Leary MM, Pasculle AW, Harrison LH. 2007. tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile. J. Clin. Microbiol. 45:215-221
11. Akerlund T, Svenungsson B, Lagergren A, Burman LG. 2006. Correlation of disease severity with fecal toxin levels in patients with Clostridium difficile-associated diarrhea and distribution of PCR ribotypes and toxin yields in vitro of corresponding isolates. J. Clin. Microbiol. 44:353-358
12. Miller M, Gravel D, Mulvey M, Taylor G, Boyd D, Simor A, Gardam M, McGeer A, Hutchinson J, Moore D, Kelly S. 2010. Health careassociated Clostridium difficile infection in Canada: patient age and infecting strain type are highly predictive of severe outcome and mortality. Clin. Infect. Dis. 50:194-201
13. de Jong E, de Jong AS, Bartels CJ, van der Rijt-van den Biggelaar C, Melchers WJ, Sturm PD. 2012. Clinical and laboratory evaluation of a real-time PCR for Clostridium difficile toxin A and B genes. Eur. J. Clin. Microbiol. Infect. Dis. 31:2219-2225
14. Davies KA, Planche T, Coen P, Crook D, Shetty N, Wren M, Wilcox MH. 2012. The largest ever study to define a testing algorithm to optimise the laboratory diagnosis of C. difficile infection, abstr LB 2817. Abstr. 22nd European Congress of Clinical Microbiology and Infectious Diseases, London, United Kingdom
15. Vohra P, Poxton IR. 2011. Comparison of toxin and spore production in clinically relevant strains of Clostridium difficile. Microbiology 157:1343-1353
16. Tenover FC, Novak-Weekley S, Woods CW, Peterson LR, Davis T, Schreckenberger P, Fang FC, Dascal A, Gerding DN, Nomura JH, Goering RV, Akerlund T, Weissfeld AS, Baron EJ, Wong E, Marlowe EM, Whitmore J, Persing DH. 2010. Impact of strain type on detection of toxigenic Clostridium difficile: comparison of molecular diagnostic and enzyme immunoassay approaches. J. Clin. Microbiol. 48:3719-3724
17. Aichinger E, Schleck CD, Harmsen WS, Nyre LM, Patel R. 2008. Nonutility of repeat laboratory testing for detection of Clostridium difficile by use of PCR or enzyme immunoassay. J. Clin. Microbiol. 46:3795-3797
18. Luo RF, Banaei N. 2010. Is repeat PCR needed for diagnosis of Clostridium difficile infection? J. Clin. Microbiol. 48:3738-3741
19. Mohan SS, McDermott BP, Parchuri S, Cunha BA. 2006. Lack of value of repeat stool testing for Clostridium difficile toxin. Am. J. Med. 119: 356.e7-e8
20. Kato H, Yokoyama T, Arakawa Y. 2005. Rapid and simple method for detecting the toxin B gene of Clostridium difficile in stool specimens by loop-mediated isothermal amplification. J. Clin. Microbiol. 43:6108-6112
21. Noren T, Alriksson I, Andersson J, Akerlund T, Unemo M. 2011. Rapid and sensitive loop-mediated isothermal amplification test for Clostridium difficile detection challenges cytotoxin B cell test and culture as gold standard. J. Clin. Microbiol. 49:710-711
22. Pancholi P, Kelly C, Raczkowski M, Balada-Llasat JM. 2012. Detection of toxigenic Clostridium difficile: comparison of the cell culture neutralization, Xpert C. difficile, Xpert C. difficile/Epi, and Illumigene C. difficile assays. J. Clin. Microbiol. 50:1331-1335
23. Longtin Y, Trottier S, Brochu G, Paquet-Bolduc B, Garenc C, Loungnarath V, Beaulieu C, Goulet D, Longtin J. 2013. Impact of the type of diagnostic assay on Clostridium difficile infection and complication rates in a mandatory reporting program. Clin. Infect. Dis. 56:67-73
24. Babady NE, Stiles J, Ruggiero P, Khosa P, Huang D, Shuptar S, Kamboj M, Kiehn TE. 2010. Evaluation of the Cepheid Xpert Clostridium difficile Epi assay for diagnosis of Clostridium difficile infection and typing of the NAP1 strain at a cancer hospital. J. Clin. Microbiol. 48:4519-4524
25. Johnson SW, Kanatani M, Humphries RM, Uslan DZ. 2012. Clinical impact of switching conventional enzyme immunoassay with nucleic acid amplification test for suspected Clostridium difficile-associated diarrhea. Am. J. Infect. Control doi 10.1016/j.ajic.2012.1004.1329.