tcdC genotypes associated with severe TcdC truncation in an epidemic clone and other strains of Clostridium difficile

Journal of Clinical Microbiology

Volumn 45, Issue 1, 2007, Pages 215-221

  Curry, Scott R ^a   Marsh, Jane W ^a   Muto, Carlene A ^a,b   O'Leary, Mary M ^a,b   Pasculle, A William ^a   Harrison, Lee H ^a,c  

^a UNIVERSITY OF PITTSBURGH SCHOOL OF MEDICINE   (United States)

^b PRESBYTERIAN UNIVERSITY HOSPITAL   (United States)

^c UNIVERSITY OF PITTSBURGH GRADUATE SCHOOL OF PUBLIC HEALTH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACID; BACTERIAL DNA; BACTERIAL PROTEIN; NUCLEOTIDE; PROTEIN TCDC; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GENE; BACTERIAL STRAIN; BACTERIUM ISOLATE; CLOSTRIDIUM DIFFICILE; EPIDEMIC; FRAMESHIFT MUTATION; GENE DELETION; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; SEQUENCE ANALYSIS; STOP CODON; TCDC GENE; TOXIN SYNTHESIS;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; BASE SEQUENCE; CLOSTRIDIUM DIFFICILE; DISEASE OUTBREAKS; ENTEROCOLITIS, PSEUDOMEMBRANOUS; GENOTYPE; HUMANS; MOLECULAR SEQUENCE DATA; PHYLOGENY; REPRESSOR PROTEINS; SEQUENCE ANALYSIS, DNA; SEQUENCE DELETION; VARIATION (GENETICS);

CLOSTRIDIUM DIFFICILE;

EID: 33846253977     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.01599-06     Document Type: Article

References (24)

1. Bartlett, J. G., T. W. Chang, M. Gurwith, S. L. Gorbach, and A. B. Onderdonk. 1978. Antibiotic-associated pseudomembranous colitis due to toxin-producing clostridia. N. Engl. J. Med. 298:531-534.
2. Bartlett, J. G., N. Moon, T. W. Chang, N. Taylor, and A. B. Onderdonk. 1978. Role of Clostridium difficile in antibiotic-associated pseudomembranous colitis. Gastroenterology 75:778-782.
3. Dallal, R. M., B. G. Harbrecht, A. J. Boujoukas, C. A. Sirio, L. M. Farkas, K. K. Lee, and R. L. Simmons. 2002. Fulminant Clostridium difficile: an underappreciated and increasing cause of death and complications. Ann. Surg. 235:363-372.
4. Govind, R., G. Vediyappan, R. D. Rolfe, and J. A. Fralick. 2006. Evidence that Clostridium difficile TcdC is a membrane-associated protein. J. Bacteriol. 188:3716-3720.
5. Hammond, G. A., and J. L. Johnson. 1995. The toxigenic element of Clostridium difficile strain VPI 10463. Microb. Pathog. 19:203-213.
6. Hammond, G. A., D. M. Lyerly, and J. L. Johnson. 1997. Transcriptional analysis of the toxigenic element of Clostridium difficile. Microb. Pathog. 22:143-154.
7. Hundsberger, T., V. Braun, M. Weidmann, P. Leukel, M. Sauerborn, and C. von Eichel-Streiber. 1997. Transcription analysis of the genes tcdA-E of the pathogenicity locus of Clostridium difficile. Eur. J. Biochem. 244:735-742.
8. Loo, V. G., L. Poirier, M. A. Miller, M. Oughton, M. D. Libman, S. Michaud, A. M. Bourgault, T. Nguyen, C. Frenette, M. Kelly, A. Vibien, P. Brassard, S. Fenn, K. Dewar, T. J. Hudson, R. Horn, P. Rene, Y. Monczak, and A. Dascal. 2005. A predominantly clonal multi-institutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N. Engl. J. Med. 353:2442-2449.
9. Lyerly, D. M., H. C. Krivan, and T. D. Wilkins. 1988. Clostridium difficile: its disease and toxins. Clin. Microbiol. Rev. 1:1-18.
10. MacCannell, D. R., T. J. Louie, D. B. Gregson, M. Laverdiere, A. C. Labbe, F. Laing, and S. Henwick. 2006. Molecular analysis of Clostridium difficile PCR ribotype 027 isolates from Eastern and Western Canada. J. Clin. Microbiol. 44:2147-2152.
11. Marsh, J. W., M. O'Leary, M., K. A. Shutt, A. W. Pasculle, S. Johnson, D. N. Gerding, C. A. Muto, and L. H. Harrison. 2006. Multilocus variable-number tandem-repeat analysis for investigation of Clostridium difficile transmission in hospitals. J. Clin. Microbiol. 44:2558-2566.
12. McDonald, L. C., G. E. Killgore, A. Thompson, R. C. Owens, Jr., S. V. Kazakova, S. P. Sambol, S. Johnson, and D. N. Gerding. 2005. An epidemic, toxin gene-variant strain of Clostridium difficile. N. Engl. J. Med. 353:2433-2441.
13. Mundy, L. S., C. J. Shanholtzer, K. E. Willard, D. N. Gerding, and L. R. Peterson. 1995. Laboratory detection of Clostridium difficile: a comparison of media and incubation systems. Am. J. Clin. Pathol. 103:52-56.
14. Musher, D. M., S. Aslam, N. Logan, S. Nallacheru, I. Bhaila, F. Borchert, and R. J. Hamill. 2005. Relatively poor outcome after treatment of Clostridium difficile colitis with metronidazole. Clin. Infect. Dis. 40:1586-1590.
15. Muto, C. A., M. Pokrywka, K. Shutt, A. B. Mendelsohn, K. Nouri, K. Posey, T. Roberts, K. Croyle, S. Krystofiak, S. Patel-Brown, A. W. Pasculle, D. L. Paterson, M. Saul, and L. H. Harrison. 2005. A large outbreak of Clostridium difficile-associated disease with an unexpected proportion of deaths and colectomies at a teaching hospital following increased fluoroquinolone use. Infect. Control Hosp. Epidemiol. 26:273-280.
16. Pepin, J., M. E. Alary, L. Valiquette, E. Raiche, J. Ruel, K. Fulop, D. Godin, and C. Bourassa. 2005. Increasing risk of relapse after treatment of Clostridium difficile colitis in Quebec, Canada. Clin. Infect. Dis. 40:1591-1597.
17. Pepin, J., L. Valiquette, M. E. Alary, P. Villemure, A. Pelletier, K. Forget, K. Pepin, and D. Chouinard. 2004. Clostridium difficile-associated diarrhea in a region of Quebec from 1991 to 2003: a changing pattern of disease severity. CMAJ 171:466-472.
18. Pepin, J., L. Valiquette, and B. Cossette. 2005. Mortality attributable to nosocomial Clostridium difficile-associated disease during an epidemic caused by a hypervirulent strain in Quebec. CMAJ 173:1037-1042.
19. Rupnik, M. 2001. How to detect Clostridium difficile variant strains in a routine laboratory. Clin. Microbiol. Infect. 7:417-420.
20. Rupnik, M., V. Avesani, M. Jane, C. von Eichel-Streiber, and M. Delmee. 1998. A novel toxinotyping scheme and correlation of toxinotypes with serogroups of Clostridium difficile isolates. J. Clin. Microbiol. 36:2240-2247.
21. Rupnik, M., J. S. Brazier, B. I. Duerden, M. Grabnar, and S. L. Stubbs. 2001. Comparison of toxinotyping and PCR ribotyping of Clostridium difficile strains and description of novel toxinotypes. Microbiology 147:439-447.
22. Soehn, F., A. Wagenknecht-Wiesner, P. Leukel, M. Kohl, M. Weidmann, C. von Eichel-Streiber, and V. Braun. 1998. Genetic rearrangements in the pathogenicity locus of Clostridium difficile strain 8864: implications for transcription, expression, and enzymatic activity of toxins A and B. Mol. Gen. Genet. 258:222-232.
23. Spigaglia, P., and P. Mastrantonio. 2002. Molecular analysis of the pathogenicity locus and polymorphism in the putative negative regulator of toxin production (TcdC) among Clostridium difficile clinical isolates. J. Clin. Microbiol. 40:3470-3475.
24. Warny, M., J. Pepin, A. Fang, G. Killgore, A. Thompson, J. Brazier, E. Frost, and L. C. McDonald. 2005. Toxin production by an emerging strain of Clostridium difficile associated with outbreaks of severe disease in North America and Europe. Lancet 366:1079-1084.