Detection of the A2058G and A2059G 23S rRNA gene point mutations associated with azithromycin resistance in Treponema pallidum by use of a TaqMan real-time multiplex PCR assay

Journal of Clinical Microbiology

Volumn 51, Issue 3, 2013, Pages 908-913

  Chen, Cheng Yen ^a   Chi, Kai Hua ^a   Pillay, Allan ^a   Nachamkin, Eli ^a   Su, John R ^a   Ballard, Ronald C ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ADENINE; AZITHROMYCIN; GUANINE; RNA 23S;

ANALYZER; ANTIBIOTIC RESISTANCE; ANTIBIOTIC SENSITIVITY; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; GENOTYPE; MULTIPLEX POLYMERASE CHAIN REACTION; MUTATIONAL ANALYSIS; NONHUMAN; POINT MUTATION; PREVALENCE; PRIORITY JOURNAL; PYROSEQUENCING; REAL TIME MULTIPLEX POLYMERASE CHAIN REACTION; TREPONEMA PALLIDUM; UNITED STATES;

ANTI-BACTERIAL AGENTS; AZITHROMYCIN; DRUG RESISTANCE, BACTERIAL; GENES, RRNA; GENOTYPE; HUMANS; MICROBIAL SENSITIVITY TESTS; MULTIPLEX POLYMERASE CHAIN REACTION; POINT MUTATION; REAL-TIME POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 23S; TREPONEMA PALLIDUM; UNITED STATES;

EID: 84874023127     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.02770-12     Document Type: Article

References (27)

1. Workowski KA, Berman S; Centers for Disease Control and Prevention (CDC). 2010. Sexually transmitted diseases treatment guidelines, 2010. MMWR Recomm. Rep. 59(RR-12):1-110
2. Hook EW, III, Stephens J, Ennis DM. 1999. Azithromycin compared with penicillin G benzathine for treatment of incubating syphilis. Ann. Intern. Med. 131:434-437
3. Hook EW, III, Martin DH, Stephens J, Smith BS, Smith K. 2002. A randomized, comparative pilot study of azithromycin versus benzathine penicillin G for treatment of early syphilis. Sex. Transm. Dis. 29:486-490
4. Stoner BP. 2007. Current controversies in the management of adult syphilis. Clin. Infect. Dis. 44:S130-S146
5. Vester B, Douthwaite S. 2001. Macrolide resistance conferred by base substitutions in 23S rRNA. Antimicrob. Agents Chemother. 45:1-12
6. Pfister P, Jenni S, Poehisgaard Thomas A, Douthwaite S, Ban N, Bottger EC. 2004. The structural basis of macrolide-ribosome binding assessed using mutagenesis of 23S rRNA positions 2058 and 2059. J. Mol. Biol. 342:1569-1581
7. Lukehart SA, Godornes C, Molini BJ, Sonnett P, Hopkins S, Mulcahy F, Engelman J, Mitchell SJ, Rompalo AM, Marra CM, Klausner JD. 2004. Macrolide resistance in Treponema pallidum in the United States and Ireland. N. Engl. J. Med. 351:154-158
8. Mitchell SJ, Engelman J, Kent CK, Lukehart SA, Godornes C, Klausner JD. 2006. Azithromycin-resistant syphilis infection: San Francisco, California, 2000-2004. Clin. Infect. Dis. 42:337-345
9. Stamm LV, Bergen HL. 2000. A point mutation associated with bacterial macrolide resistance is present in both 23S rRNA genes of an erythromycin-resistant Treponema pallidum clinical isolate. Antimicrob. Agents Chemother. 44:806-807
10. Matejkova P, Flasarova M, Zakoucka H, Borek M, Kremenova S, Arenberger P, Woznicova V, Weistock GM, Smajs D. 2009. Macrolide treatment failure in a case of secondary syphilis: a novel A2059G mutation in 23S rRNA gene of Treponema pallidum subsp. pallidum. J. Med. Microbiol. 58:832-836
11. Martin IE, Gu W, Yang Y, Tsang RSW. 2009. Macrolide resistance and molecular types of Treponema pallidum causing primary syphilis in Shanghai, China. Clin. Infect. Dis. 49:515-521
12. Martin IE, Tsang RSW, Sutherland K, Tilley P, Read R, Anderson B, Roy C, Singh AE. 2009. Molecular characterization of syphilis in patients in Canada: azithromycin resistance and detection of Treponema pallidum DNA in whole-blood samples versus ulcerative swabs. J. Clin. Microbiol. 47:1668-1673
13. Morshed MG, Jones HD. 2006. Treponema pallidum macrolide resistance in BC. CMAJ 174:349. (Letter.)
14. Muldoon EG, Walsh A, Crowley B, Mulcahy F. 2012. Treponema pallidum azithromycin resistance in Dublin, Ireland. Sex. Transm. Dis. 39: 784-786
15. Tipple C, McClure M, Taylor GP. 2011. High prevalence of macrolide resistance in Treponema pallidum strains in a London centre. Sex. Transm. Infect. 87:486-488
16. Katz KA, Klausner JD. 2008. Azithromycin resistance in Treponema pallidum. Curr. Opin. Infect. Dis. 21:83-91
17. Katz KA, Pillay A, Ahrens K, Kohn RP, Hermanstyne K, Bernstein KT, Ballard RC, Klausner JD. 2010. Molecular epidemiology of syphilis-San Francisco, 2004-2007. Sex. Transm. Dis. 37:660-663
18. Marra CM, Colina AP, Godornes C, Tantalo LC, Puray M, Centurion-Lara A, Lukehart SA. 2006. Antibiotic selection may contribute to increases in macrolide-resistant Treponema pallidum. J. Infect. Dis. 194: 1771-1773
19. Kiddugavu MG, Kiwanuka N, Wawer MJ, Serwadda D, Sewankambo NK, Wabwire-Mangen F, Makumbi F, Li X, Reynolds SJ, Quinn TC, Gray RH, Rakai Study Group. 2005. Effectiveness of syphilis treatment using azithromycin and/or benzathine penicillin in Rakai, Uganda. Sex. Transm. Dis. 32:1-6
20. Müller EE, Paz-Bailey G, Lewis DA. 2012. Macrolide resistance testing and molecular subtyping of Treponema pallidum strains from southern Africa. Sex. Transm. Infect. 88:470-474
21. Riedner G, Rusizoka M, Todd J, Maboko L, Hoelscher M, Mmbando D, Samky E, Lyamuya E, Mabey D, Grosskurth H, Hayes R. 2005. Singledose azithromycin versus penicillin G benzathine for the treatment of early syphilis. N. Engl. J. Med. 353:1236-1244
22. Van Damme K, Behets F, Ravelomanana N, Godornes C, Khan M, Randrianasolo B, Rabenja NY, Lukehar S, Cohen M, Hook E. 2009. Evaluation of azithromycin resistance in Treponema pallidum specimens from Madagascar. Sex. Transm. Dis. 36:775-776
23. Wu H, Chang SY, Lee NY, Huang WC, Wu BR, Yang CJ, Liang SH, Lee CH, Ko WC, Lin HH, Chen YH, Liu WC, Su YC, Hsieh CY, Wu PY, Hung CC. 2012. Evaluation of macrolide resistance and enhanced molecular typing of Treponema pallidum in patients with syphilis in Taiwan: a prospective multicenter study. J. Clin. Microbiol. 50:2299-2304
24. Pandori MW, Godornes C, Castro L, Engelman J, Siedner M, Lukehart S, Klausner J. 2007. Detection of azithromycin resistance in Treponema pallidum by real-time PCR. Antimicrob. Agents Chemother. 51:3425-3430
25. Chen CY, Chi KH, George RW, Cox DL, Srivastava A, Silva MR, Carneiro F, Lauwers GY, Ballard RC. 2006. Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing. J. Clin. Microbiol. 44:3452-3456
26. A2058G Prevalence Workgroup. 2012. Prevalence of the 23S rRNA A2058G point mutation and molecular subtypes in Treponema pallidum in the United States, 2007 to 2009. Sex. Transm. Dis. 39:794-798
27. Grimes M, Sahi SK, Godornes C, Tantalo LC, Roberts N, Bostick D, Marra CM, Lukehart SA. 2012. Two mutations associated with macrolide resistance in Treponema pallidum: increasing prevalence and correlation with molecular strain type in Seattle, Washington. Sex. Transm. Dis. 39: 954-958.