Diagnosis of gastric syphilis by direct immunofluorescence staining and real-time PCR testing

Journal of Clinical Microbiology

Volumn 44, Issue 9, 2006, Pages 3452-3456

  Chen, Cheng Yen ^a   Chi, Kai Hua ^a   George, Robert W ^a   Cox, David L ^a   Srivastava, Amitabh ^b   Rui Silva, Mário ^c   Carneiro, Fátima ^d   Lauwers, Gregory Y ^b   Ballard, Ronald C ^a  

^a CENTERS FOR DISEASE CONTROL AND PREVENTION   (United States)

^b MASSACHUSETTS GENERAL HOSPITAL   (United States)

^c CENTRO HOSPITALAR E UNIVERSITÁRIO DE COIMBRA   (Portugal)

^d University of Porto   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

BACTERIAL DNA; EVANS BLUE; FLUORESCEIN ISOTHIOCYANATE; MONOCLONAL ANTIBODY;

ADULT; ARTICLE; BACTERIUM IDENTIFICATION; CASE REPORT; CHRONIC DISEASE; CONTROLLED STUDY; DNA SEQUENCE; DYSPEPSIA; GASTRITIS; HUMAN; HUMAN TISSUE; IMMUNOFLUORESCENCE TEST; INFLAMMATION; MALE; NONHUMAN; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; SERODIAGNOSIS; SPIROCHETE; STOMACH DISEASE; STOMACH MUCOSA; STOMACH SYPHILIS; SYPHILIS; TREPONEMA;

ADULT; DNA, BACTERIAL; FLUORESCENT ANTIBODY TECHNIQUE, DIRECT; GASTRIC MUCOSA; HUMANS; MALE; POLYMERASE CHAIN REACTION; STAINING AND LABELING; STOMACH DISEASES; SYPHILIS; TREPONEMA PALLIDUM;

EID: 33748765277     PISSN: 00951137     EISSN: None     Source Type: Journal    
DOI: 10.1128/JCM.00721-06     Document Type: Article

References (10)

1. Antal, G. M., S. A. Lukehart, and A. Z. Meheus. 2002. The endemic treponematoses. Microbes Infect. 4:83-94.
2. Guerrero, A. F., T. M. Straight, J. Eastone, and K. Spooner. 2005. Gastric syphilis in an HIV-infected patient. AIDS Patient Care STDs 19:281-285.
3. Ikebe, M., T. Oiwa, M. Mori, H. Kuwan, K. Sugimachi, and T. Yao. 1994. Gastric syphilis: case report and review of the literature. Radiat. Med. 12:171-175.
4. Inagaki, H., T. kawai, M. Miyata, S. Nagaya, H. Tateyama, and T. Eimoto. 1996. Gastric syphilis: polymerase chain reaction detection of treponemal DNA in pseudolymphomatous lesions. Hum. Pathol. 27:761-765.
5. Ito, F., E. F. Hunter, R. W. George, V. Pope, and S. A. Larsen. 1992. Specific immunofluorescent staining of pathogenic treponemes with a monoclonal antibody. J. Clin. Microbiol. 30:831-838.
6. Liu, H., B. Rodes, C.-Y. Chen, and B. Steiner. 2001. New tests for syphilis: rational design of a PCR method for detection of Treponema pallidum in clinical specimens using unique regions of the DNA polymerase I gene. J. Clin. Microbiol. 39:1941-1946.
7. Magnarelli, L., J. F. Anderson, and R. C. Johnson. 1987. Cross-reactivity in serological tests for Lyme disease and other spirochetal infections. J. Infect. Dis. 156:183-188.
8. Norris, S., and S. Sell. 1996. Role of polymerase chain reaction in the diagnosis of gastric syphilis. Hum. Pathol. 27:749-750.
9. Orle, K. A., C. A. Gates, D. H. Martin, B. A. Body, and J. B. Weiss. 1996. Simultaneous PCR detection of Haemophilus ducreyi, Treponema pallidum, and herpes simplex virus type 1 and 2 from genital ulcers. J. Clin. Microbiol. 34:49-54.
10. Winters, H. A., V. Notar-Francesco, K. Bromberg, S. A. Rawstom, J. Vetrano, V. Prego, J. Kuan, and J.-P. Raufman. 1992. Gastric syphilis: five recent cases and a review of the literature. Ann. Intern. Med. 116:314-319.