Cytoplasmic expression, antibody production, and characterization of the novel zinc finger protein 637

Applied Microbiology and Biotechnology

Volumn 97, Issue 2, 2013, Pages 741-749

  Li, Kai ^a   Wei, Yuyan ^a   Zhang, Jie ^a   Li, Jun ^a   Gao, Bo ^a   Huang, Lugang ^a   Lin, Ping ^a   Wei, Yuquan ^a  

^a SICHUAN UNIVERSITY   (China)

Author keywords

Anti zfp637 specific antibody; Cytoplasmic expression; Oxidative stress; Recombinant protein; Zfp637

Indexed keywords

ANTIBODY PRODUCTION; BACTERIAL CULTURES; CELL LINES; CELL NUCLEUS; CYTOPLASMIC EXPRESSION; DNA-BINDING PROTEIN; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI BL21; EXPRESSION LEVELS; EXPRESSION SYSTEM; FUSION PROTEINS; GENE TRANSCRIPTIONS; GLUTATHIONE-S-TRANSFERASE; IMMUNOHISTOCHEMISTRY; NIH-3T3 CELLS; POLYCLONAL ANTIBODY; PROTEIN FAMILY; STRUCTURAL CHARACTERIZATION; TIME-DEPENDENT; WESTERN BLOTTING; ZFP637; ZINC FINGER MOTIFS; ZINC FINGER PROTEIN;

ANTIBODIES; CELL CULTURE; ESCHERICHIA COLI; NUCLEIC ACIDS; OXIDATIVE STRESS; PURIFICATION; TISSUE; TRANSCRIPTION; ZINC;

RECOMBINANT PROTEINS;

GLUTATHIONE S TRANSFERASE ZINC FINGER PROTEIN 637 ANTIBODY; GLUTATHIONE S TRANSFERASE ZINC FINGER PROTEIN 637 FUSION PROTEIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; MESSENGER RNA; PROTEIN ANTIBODY; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG; ZINC FINGER PROTEIN;

ANTIBODY; DNA; GENE EXPRESSION; MAMMAL; MICROBIAL COMMUNITY; OPTIMIZATION; PROTEIN; RNA; ZINC;

ANIMAL EXPERIMENT; ANTIBODY PRODUCTION; ARTICLE; BACTERIAL CELL; CELL NUCLEUS; CONTROLLED STUDY; CYTOPLASM; CYTOPLASMIC EXPRESSION; ENZYME LINKED IMMUNOSORBENT ASSAY; ESCHERICHIA COLI; GENETIC TRANSFECTION; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; IMMUNOREACTIVITY; MALE; MOUSE; NONHUMAN; OXIDATIVE STRESS; PROCESS OPTIMIZATION; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PURIFICATION; WESTERN BLOTTING;

ANIMALS; ANTIBODY FORMATION; CELL LINE; CYTOPLASM; DNA-BINDING PROTEINS; HUMANS; MALE; MICE; MICE, INBRED C57BL; NIH 3T3 CELLS; RECOMBINANT FUSION PROTEINS; ZINC FINGERS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; ORYCTOLAGUS CUNICULUS;

EID: 84873989922     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4235-5     Document Type: Article

References (33)

1. Beyer TA, Xu W, Teupser D, Auf dem Keller U, Bugnon P, Hildt E, Thiery J, Kan YW, Werner S (2008) Impaired liver regeneration in Nrf2 knockout mice: role of ROS-mediated insulin/IGF-1 resistance. EMBO J 27:212-223. doi: 10.1038/sj.emboj.7601950
2. Cheng CH, Lee WC (2010) Protein solubility and differential proteomic profiling of recombinant Escherichia coli overexpressing double-tagged fusion proteins. Microb Cell Fact 9:63. doi: 10.1186/1475-2859-9-63
3. Davis GD, Elisee C, Newham DM, Harrison RG (1999) New fusion protein systems designed to give soluble expression in Escherichia coli. Biotechnol Bioeng 65:382-388. doi:10.1002/(SICI)1097-0290(19991120)65:4<382::AID- BIT2>3.0.CO;2-I
4. Dergousova NI, Amerik AY, Volynskaya AM, Rumsh LD (1996) HIV-I protease. Cloning, expression, and purification. Appl Biochem Biotechnol 61:97-107. doi: 10.1007/BF02785692
5. Deschênes F, Massip L, Garand C, Lebel M (2005) In vivo misregulation of genes involved in apoptosis, development and oxidative stress in mice lacking both functional Werner syndrome protein and poly(ADP-ribose) polymerase-1. Hum Mol Genet 14:3293-3308. doi: 10.1093/hmg/ddi362
6. Dümmler A, Lawrence A, de Marco A (2005) Simplified screening for the detection of soluble fusion constructs expressed in E. coli using a modular set of vectors. Microb Cell Fact 4:34. doi: 10.1186/1475-2859-4-34
7. Frangioni JV, Neel BG (1993) Solubilization and purification of enzymatically active glutathione S-transferase (pGEX) fusion proteins. Anal Biochem 210:179-187. doi: 10.1006/abio.1993.1170
8. Harper S, Speicher DW (2011) Purification of proteins fused to glutathione S-transferase. Methods Mol Biol 681:259-280. doi: 10.1007/978-1-60761-913-0-14
9. Hartman J, Daram P, Frizzell RA, Rado T, Benos DJ, Sorscher EJ (1992) Affinity purification of insoluble recombinant fusion proteins containing glutathione-S-transferase. Biotechnol Bioeng 39:828-832. doi: 10.1002/bit.260390805
10. Higashi Y, Asanuma M, Miyazaki I, Haque ME, Fujita N, Tanaka K, Ogawa N (2002) The p53-activated gene, PAG608, requires a zinc finger domain for nuclear localization and oxidative stress-induced apoptosis. J Biol Chem 277:42224-42232. doi: 10.1074/jbc.M203594200
11. Ideno A, Furutani M, Iwabuchi T, Iida T, Iba Y, Kurosawa Y, Sakuraba H, Ohshima T, Kawarabayashi Y, Maruyama T (2004) Expression of foreign proteins in Escherichia coli by fusing with an archaeal FK506 binding protein. Appl Microbiol Biotechnol 64:99-105. doi: 10.1007/s00253-003-1459-4
12. Kapust RB, Waugh DS (1999) Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 8:1668-1674. doi: 10.1110/ps.8.8.1668
13. Kiefhaber T, Rudolph R, Kohler HH, Buchner J (1991) Protein aggregation in vitro and in vivo: a quantitative model of the kinetic competition between folding and aggregation. Biotechnology (N Y) 9:825-829. doi: 10.1038/nbt0991-825
14. Kovaleva V, Krynytskyy H, Gout I, Gout R (2011) Recombinant expression, affinity purification and functional characterization of Scots pine defensin 1. Appl Microbiol Biotechnol 89:1093-1101. doi: 10.1007/s00253-010-2935-2
15. LaVallie ER, DiBlasio EA, Kovacic S, Grant KL, Schendel PF, McCoy JM (1993) A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm. Bio Technol 11:187-193. doi: 10.1038/nbt0293-187
16. Li K, Ren JJ, Zhang J, Liu K, Qi YY, Wang XJ, Xiao HY, Lin P (2009) Construction of eukaryotic expression plasmid for a novel zinc finger protein gene Zfp637 and its effect on breast carcinoma cell EMT6. Sichuan Da Xue Xue Bao Yi Xue Ban 40:575-578, 603
17. Li K, Zhang J, Ren JJ, Wang Q, Yang KY, Xiong ZJ, Mao YQ, Qi YY, Chen XW, Lan F, Wang XJ, Xiao HY, Lin P, Wei YQ (2010) A novel zinc finger protein Zfp637 behaves as a repressive regulator in myogenic cellular differentiation. J Cell Biochem 110:352-362. doi: 10.1002/jcb.22546
18. Nojiri H, Shimizu T, Funakoshi M, Yamaguchi O, Zhou H, Kawakami S, Ohta Y, Sami M, Tachibana T, Ishikawa H, Kurosawa H, Kahn RC, Otsu K, Shirasawa T (2006) Oxidative stress causes heart failure with impaired mitochondrial respiration. J Biol Chem 281:33789-33801. doi: 10.1074/jbc.M602118200
19. Nygren P-A, Stahl S, Uhlén M (1994) Engineering proteins to facilitate bioprocessing. Trends Biotechnol 12:184-188. doi: 10.1016/0167-7799(94)90080-9
20. Park HM, Kim GY, Nam MK, Seong GH, Han C, Chung KC, Kang S, Rhim H (2009) The serine protease HtrA2/Omi cleaves Parkin and irreversibly inactivates its E3 ubiquitin ligase activity. Biochem Biophys Res Commun 387:537-542. doi: 10.1016/j.bbrc.2009.07.079
21. Rizhsky L, Davletova S, Liang H, Mittler R (2004) The zinc finger protein Zat12 is required for cytosolic ascorbate peroxidase 1 expression during oxidative stress in Arabidopsis. J Biol Chem 279:11736-11743. doi: 10.1074/jbc.M313350200
22. Ryu H, Lee J, Zaman K, Kubilis J, Ferrante RJ, Ross BD, Neve R, Ratan RR (2003) Sp1 and Sp3 are oxidative stress-inducible, antideath transcription factors in cortical neurons. J Neurosci 23:3597-3606
23. Schultz T, Martinez L, de Marco A (2006) The evaluation of the factors that cause aggregation during recombinant expression in E. coli is simplified by the employment of an aggregation-sensitive reporter. Microb Cell Fact 5:28. doi: 10.1186/1475-2859-5-28
24. Seong YM, Choi JY, Park HJ, Kim KJ, Ahn SG, Seong GH, Kim IK, Kang S, Rhim H (2004) Autocatalytic processing of HtrA2/Omi is essential for induction of caspase-dependent cell death through antagonizing XIAP. J Biol Chem 279:37588-37596. doi: 10.1074/jbc.M401408200
25. Smith DB, Johnson KS (1988) Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase. Gene 67:31-40. doi: 10.1016/0378-1119(88)90005-4
26. Smith DB, Davern KM, Board PG, Tiu WU, Garcia EG, Mitchell GF (1986) Mr 26,000 antigen of Schistosoma japonicum recognized by resistant WEHI 129/J mice is a parasite glutathione S-transferase. Proc Natl Acad Sci U S A 83:8703-8707. doi: 10.1073/pnas.83.22.8703
27. Sørensen HP, Mortensen KK (2005) Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microb Cell Fact 4:1. doi: 10.1186/1475-2859-4-1
28. Tait AR, Straus SK (2011) Overexpression and purification of U24 from human herpesvirus type-6 in E. coli: unconventional use of oxidizing environments with a maltose binding protein-hexahistine dual tag to enhance membrane protein yield. Microb Cell Fact 10:51. doi: 10.1186/1475-2859-10-51
29. Villaverde A, Carrió MM (2003) Protein aggregation in recombinant bacteria: biological role of inclusion bodies. Biotechnol Lett 25:1385-1395. doi: 10.1023/A:1025024104862
30. Volontè F, Piubelli L, Pollegioni L (2011) Optimizing HIV-1 protease production in Escherichia coli as fusion protein. Microb Cell Fact 10:53. doi: 10.1186/1475-2859-10-53
31. Xu J, Li W, Wu J, Zhang Y, Zhu Z, Liu J, Hu Z (2006) Stability of plasmid and expression of a recombinant gonadotropin-releasing hormone (GnRH) vaccine in Escherichia coli. Appl Microbiol Biotechnol 73:780-788. doi: 10.1007/s00253-006-0547-7
32. Ye T, Lin Z, Lei H (2008) High-level expression and characterization of an anti-VEGF165 single-chain variable fragment (scFv) by small ubiquitin-related modifier fusion in Escherichia coli. Appl Microbiol Biotechnol 81:311-317. doi: 10.1007/s00253-008-1655-3
33. Younce CW, Kolattukudy PE (2010) MCP-1 causes cardiomyoblast death via autophagy resulting from ER stress caused by oxidative stress generated by inducing a novel zinc-finger protein, MCPIP. Biochem J 426:43-53. doi: 10.1042/BJ20090976