Recombinant expression, affinity purification and functional characterization of Scots pine defensin 1

Applied Microbiology and Biotechnology

Volumn 89, Issue 4, 2011, Pages 1093-1101

  Kovaleva, Valentina ^a   Krynytskyy, Hryhoriy ^a   Gout, Ivan ^b,c   Gout, Roman ^a  

^a UKRAINIAN NATIONAL FORESTRY UNIVERSITY   (Ukraine)

^b INSTITUTE OF MOLECULAR BIOLOGY AND GENETICS   (Ukraine)

^c UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

Antifungal activity; Antimicrobial peptides; PsDef1 specific antibodies; Recombinant PsDef1; Scots pine defensin

Indexed keywords

ANTI-FUNGAL ACTIVITY; ANTIMICROBIAL PEPTIDE; PSDEF1 SPECIFIC ANTIBODIES; RECOMBINANT PSDEF1; SCOTS PINE;

AMINO ACIDS; ANTIBODIES; CLONING; FUNGI; MASS SPECTROMETRY; MICROORGANISMS; PEPTIDES; PURIFICATION;

FORESTRY;

AMINO ACID; BLOOD CLOTTING FACTOR 10A; DEFENSIN; GLUTATHIONE TRANSFERASE; HYBRID PROTEIN; PINUS SYLVESTRIS DEFENSIN 1; POLYCLONAL ANTIBODY; RECOMBINANT PROTEIN; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ANTIMICROBIAL ACTIVITY; CONIFEROUS TREE; DISEASE RESISTANCE; FUNGUS; GENE EXPRESSION; MASS SPECTROMETRY; PEPTIDE; PHYTOCHEMISTRY; PLANT DEFENSE; PROTEIN; SEEDLING;

ANTIFUNGAL ACTIVITY; ARTICLE; FUNGUS; GERMINATION; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; OPEN READING FRAME; PROTEIN ANALYSIS; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SCOTS PINE; SEQUENCE ANALYSIS;

ANTI-INFECTIVE AGENTS; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; DEFENSINS; FUNGI; GENE EXPRESSION; MICROBIAL SENSITIVITY TESTS; MOLECULAR WEIGHT; PINUS; PLANT PROTEINS; PROTEIN SORTING SIGNALS; RECOMBINANT FUSION PROTEINS;

FUNGI; HETEROBASIDION ANNOSUM; PINUS SYLVESTRIS;

EID: 79952509829     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2935-2     Document Type: Article

References (37)

1. Almeida MS, Cabral KM, Kurtenbach E, Almeida FC, Valente AP (2002) Solution structure of Pisum sativum defensin 1 by high resolution NMR: plant defensins, identical backbone with different mechanisms of action. J Mol Biol 315:749-757. doi:10.1006/jmbi.2001.5252 (Pubitemid 34729323)
2. Asiegbu FO (2000) Adhesion and development of the root rot fungus (Heterobasidion annosum) on conifer tissues: effects of spore and host surface constituents. FEMS Microbiol Ecol 33:101-110. doi:10.1111/j.1574-6941.2000. tb00732.x
3. Benko-Iseppon AM, Galdino SL, Calsa TJ, Kido EA, Tossi A, Belarmino LC, Crovella S (2010) Overview on plant antimicrobial peptides. Curr Prot Pept Sci 11:181-188. doi:10.2174/138920310791112075
4. Bloch CJ, Richardson MA (1991) A new family of small (5 kDa) protein inhibitors of insect α-amylases from seeds of sorghum (Sorghum bicolor (L.) Moench) have sequence homologies with wheat ã-purothionins. FEBS 279:101-104. doi:10.1016/0014-5793(91)80261-Z
5. Broekaert WF, Terras F, Cammue B, Vandereyden J (1990) An automated quantative assay for fungal growth inhibition. FEMS Microbiol Lett 69:55-60. doi:0.1111/j.1574-6968.1990.tb04174.x
6. Broekaert WF, Cammue B, DeBolle M, Thevissen K, DeSamblanx G, Osborn R (1997) Antimicrobial peptides from plants. Crit Rev Plant Sci 16:297-323. doi:10.1080/713608148 (Pubitemid 127468279)
7. Carvalho AO, Gomes VM (2009) Plant defensins - prospects for the functional and biotechnological properties. Peptides 30:1007-1020. doi:10.1016/j.peptides.2009.01.018
8. Da-Hui L, Gui-Liang J, Ying-Tao Z, Tie-Min A (2007) Bacterial expression of a Trichosanthes kirilowii defensin (TDEF1) and its antifungal activity on Fusarium oxysporum. Appl Microbiol Biotech 74:146-151. doi:10.1007/s00253-006- 0631-z (Pubitemid 46183479)
9. De Samblanx GW, Goderis I, Thevissen K, Raemaekers R, Fant F, Borremans F, Acland DP, Osborn R, Pateli S, Broekaert WF (1997) Mutational analysis of plant defensin from radish (Raphanus sativus L.) reveals two adjacent sites important for antifungal activity. J Biol Chem 272:1171-1179. doi:10.1074/jbc.272.2.1171 (Pubitemid 27034614)
10. Fant F, Vranken W, Broekaert WF, Borremans F (1998) Determination of the three dimensional solution structure of Raphanus sativus antifungal protein 1 by 1 H NMR. J Mol Biol 279:257-270. doi:10.1006/jmbi.1998.1767 (Pubitemid 28252144)
11. Fossdal CG, Nagy NE, Sharma P, Länneborg A (2003) The putative gymnosperm plant defensin polypeptide (SPI1) accumulates after seed germination, is not readily released, and the SPI1 levels are reduced in Pythium dimorphum-infected spruce roots. Plant Mol Biol 52:291-302. doi:10.1023/A: 1023915230129 (Pubitemid 36761645)
12. Gao AG, Hakimi SM, Mittanck CA, Wu Y, Woerner BM, Stark DM, Shah DM, Liang J, Rommens CM (2000) Fungal pathogen protection in potato by expression of a plant defensin peptide. Nat Biotechnol 18:1307-1310. doi:10.1038/82436
13. Garcia-Olmedo F, Molina A, Alamillo JM, Rodriguez-Palenzuela P (1998) Plant defense peptides. Biopolymers 47:479-491. doi:10.1186/gb-2007-8-9-225
14. Kaomek M, Mizuno K, Fujimura T, Sriyotha P, Cairns JR (2003) Cloning, expression, and characterization of an antifungal chitinase from Leucaena leucocephala de Wit. Biosci Biotechnol Biochem 67:667-676. doi:10.1271/bbb.67. 667 (Pubitemid 39251893)
15. Kovaleva V, Gout R (2007) Isolation of the proteins with antifungal activity from Pinus sylvestris L. seedlings. Physiol Biochem Cultiv Plants 39:114-120
16. Kovaleva V, Kiyamova R, Cramer R, Krynytskyy H, Gout I, Filonenko V, Gout R (2009) Purification and molecular cloning of antimicrobial peptides from Scots pine seedlings. Peptides 30:2136-2143. doi:10.1016/j.peptides.2009.08.007
17. Kristensen A, Brunstedt J, Nielsen J, Mikkelsen J, Roepstorff P, Nielsen K (1999) Processing, disulfide pattern, and biological activity of a sugar beet defensin, AX2, expressed in Pichia pastoris. Protein Expr Purif 16:377-387. doi:10.1006/prep.1999.1085 (Pubitemid 29375391)
18. Kushmerick C, Castro MS, Cruz JS, Bloch CJ, Beiraio PSL (1998) Functional and structural features of γ-zeathionins, a new class of sodium channel blockers. FEBS Lett 440:302-306. doi:10.1016/S0014-5793(98)01480-X (Pubitemid 29052303)
19. Lam YW, Wang HX, Ng TB (2000) A robust cysteine-deficient chitinase-like antifungal protein from inner shoots of the edible chive Allium tuberosum. Biochem Biophys Res Commun 279:74-80. doi:10.1006/bbrc.2000.3821
20. Lay FT, Anderson MA (2005) Defensins - components of the innate immune system in plants. Curr Prot Pept Sci 6:85-101. doi:10.2174/1389203053027575 (Pubitemid 40259804)
21. Li G, Asiegbu FO (2004) Use of Scots pine seedling roots as an experimental model to investigate gene expression during interaction with the conifer pathogen Heterobasidion annosum (P-type). J Plant Res 117:155-162. doi:10.1007/s10265-003-0140-4 (Pubitemid 38642037)
22. Lobo DS, Pereira IB, Fragel-Madeira L, Medeiros LN, Cabral LM, Faria J, Bellio M, Campos RC, Linden R, Kurtenbach E (2007) Antifungal Pisum sativum defensin 1 interacts with Neurospora crassa cyclin F related to the cell cycle. Biochemistry 46:987-996. doi:10.1021/bi061441j (Pubitemid 46184986)
23. Mendez E, Rocher A, Calero M, Girbes T, Citores L, Soriano F (1996) Primary structure of ω-hordothionin, a member of a novel family of thionins from barley endosperm, and its inhibition of protein synthesis in eukaryotic and prokaryotic cell-free systems. Eur J Biochem 239:67-73. doi:10.1111/j.1432-1033.1996.0067u.x (Pubitemid 26228141)
24. Mirouze M, Sels J, Richard O, Czernic P, Loubet S, Jacquier A, Franciois I, Cammue BPA, Lebrun M, Berthomieu P, Marques L (2006) A putative novel role for plant defensins: a defensin from the zinc hyper-accumulating plant, Arabidopsis halleri, confers zinc tolerance. Plant J 47:329-342. doi:10.1111/j.1365-313X.2006.02788.x (Pubitemid 44036403)
25. Osborn RW, Desamblanx GW, Thevissen K, Goderis I, Torrekenes S, vanLeuven F, Attenborough S, Rees SB, Broekaert WF (1995) Isolation and characterization of plant defensins from seeds of Asteracea, Fabaceae, Hippocastanaceae and Saxifragaceae. FEBS Lett 368:257-262. doi:10.1016/0014-5793(95)00666-W
26. Osbourn AE (1999) Antimicrobial phytoprotectants and fungal pathogens: a commentary. Fungal Genet Biol 26:163-168. doi:10.1006/fgbi.1999.1133
27. Pelegrini PB, Lay FT, Murad AM, Anderson MA, Franco OL (2008) Novel insights on the mechanism of action of α-amylase inhibitors from the plant defensin family. Proteins 73:719-729. doi:10.1002/prot.22086 (Pubitemid 352788653)
28. Pervieux I, Bourassa M, Laurans F, Hamelin R, Seguin A (2004) A spruce defensin showing strong antifungal activity and increased transcript accumulation after wounding and jasmonate treatments. Physiol Mol Plant Pathol 64:331-341. doi:10.1016/j.pmpp.2004.09.008 (Pubitemid 40191617)
29. Schägger H, von Jagow G (1987) Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1-100 kDalton. Anal Biochem 166:368-379. doi:10.1016/0003-2697(87) 90587-2 (Pubitemid 18004907)
30. Spelbrink RG, Dilmac N, Allen A, Smith TJ, Shah DM, Hockerman GH (2004) Differential antifungal and calcium channel-blocking activity among structurally related plant defensins. Plant Physiol 135:2055-2067. doi:10.1104/pp.104.040873 (Pubitemid 39182785)
31. Thevissen K, Ferket KK, Francois IE, Cammue BP (2003) Interactions of antifungal plant defensins with fungal membrane components. Peptides 24:1705-1712. doi:10.1016/j.peptides.2003.09.014 (Pubitemid 38198077)
32. Thevissen K, Warnecke DC, Francois IE, Leipelt M, Heinz E, Ott C, Zahringer U, Thomma BP, Ferket KK, Cammue BP (2004) Defensins from insects and plants interact with fungal glucosylceramides. J Biol Chem 279:3900-3905. doi:10.1074/jbc.M311165200 (Pubitemid 38198972)
33. Thomma BP, Cammue BP, Thevissen K (2002) Plant defensins. Planta 216:193-202. doi:10.1007/s00425-002-0902-6 (Pubitemid 36761777)
34. van der Weerden NL, Lay FT, Anderson MA (2008) The plant defensin, NaD1, enters the cytoplasm of Fusarium oxysporum hyphae. J Biol Chem 283:14445-14452. doi:10.1074/jbc.M709867200
35. van Loon LC, Rep M, Pieterse CMJ (2006) Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44:135-162. doi:10.1146/annurev.phyto.44.070505.143425 (Pubitemid 44435701)
36. Wang Y, Nowak G, Culley D, Hadwiger LA, Fristensky B (1999) Constitutive expression of a pea defense gene DRR206 confers resistance to blackleg (Leptosphaeria maculans) disease in transgenic canola (Brassica napus). Mol Plant Microb Interact 12:410-418. doi:10.1094/MPMI.1999.12.5.410 (Pubitemid 29194711)
37. Zelicourt A, Letousey P, Thoiron S, Campion C, Simoneau P, Elmorjani K, Marion D, Simier P, Delavault P (2007) Ha-DEF1, a sunflower defensin, induces cell death in Orobanche parasitic plants. Planta 226:591-600. doi:10.1007/s00425-007-0507-1 (Pubitemid 47038008)