Stepwise conversion of a binding protein to a fluorescent switch: Application to Thermoanaerobacter tengcongensis ribose binding protein

Biochemistry

Volumn 52, Issue 4, 2013, Pages 600-612

  Ha, Jeung Hoi ^a   Shinsky, Stephen A ^a   Loh, Stewart N ^a  

^a State University of New York Upstate Medical University: College of Medicine   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING PROTEINS; CARBOXY-TERMINAL SEGMENT; CIRCULAR PERMUTATION; CONFORMATIONAL CHANGE; CONFORMATIONAL SHIFTS; FLUORESCENT SWITCHES; KINETIC BARRIER; MOLECULAR SWITCHES; PROTEIN ENGINEERING; REFOLDING; RIBOSE-BINDING PROTEINS; SWITCHING MECHANISM; WILD TYPES;

CONFORMATIONS; FLUORESCENCE; FLUOROPHORES; GENETIC ENGINEERING; MOLECULES;

PROTEINS;

AMINO ACID; BINDING PROTEIN; RIBOSE; RIBOSE BINDING PROTEIN; UNCLASSIFIED DRUG;

ALTERNATE FRAME FOLDING; ARTICLE; CONFORMATION; CONTROLLED STUDY; FLUORESCENCE; KINETICS; NONHUMAN; PRIORITY JOURNAL; PROTEIN ENGINEERING; RIBOSWITCH; THERMOANAEROBACTER; THERMOANAEROBACTER TENGCONGENSIS; THERMODYNAMICS;

AMINO ACID SUBSTITUTION; BACTERIAL PROTEINS; BIOSENSING TECHNIQUES; FLUORESCENCE RESONANCE ENERGY TRANSFER; FLUORESCENT DYES; KINETICS; LUMINESCENT PROTEINS; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PERIPLASMIC BINDING PROTEINS; PROTEIN ENGINEERING; PROTEIN STABILITY; PROTEIN STRUCTURE, QUATERNARY; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; PROTEOLYSIS; RIBOSE; THERMOANAEROBACTER; TRANSITION TEMPERATURE; TRYPSIN;

EID: 84873867172     PISSN: 00062960     EISSN: 15204995     Source Type: Journal    
DOI: 10.1021/bi301105u     Document Type: Article

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