Effects of the length of a glycine linker connecting the N- and C-termini of a circularly permuted dihydrofolate reductase

Protein Engineering

Volumn 11, Issue 8, 1998, Pages 707-713

  Iwakura, Masahiro ^a   Nakamura, Tsutomu ^a  

^a NATL INST BIOSCI HUM TECHNOL   (Japan)

Author keywords

Circular permutation; Dihydrofolate reductase; Glycine linker; M20 loop; Protein stability

Indexed keywords

DIHYDROFOLATE REDUCTASE; DIHYDROFOLIC ACID; GLYCINE; MUTANT PROTEIN; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE; UREA;

AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CIRCULAR DICHROISM; CONTROLLED STUDY; ENZYME CONFORMATION; ENZYME DENATURATION; ENZYME KINETICS; ENZYME STABILITY; ENZYME STRUCTURE; ESCHERICHIA COLI; GEL FILTRATION CHROMATOGRAPHY; NONHUMAN; PRIORITY JOURNAL; STRUCTURE ACTIVITY RELATION;

BASE SEQUENCE; CHROMATOGRAPHY, GEL; CIRCULAR DICHROISM; ENZYME STABILITY; GLYCINE; LEUCINE; METHIONINE; MOLECULAR SEQUENCE DATA; MUTATION; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; PROTEIN DENATURATION; RECOMBINANT PROTEINS; TETRAHYDROFOLATE DEHYDROGENASE; UREA;

ESCHERICHIA COLI;

EID: 0031815941     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

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