Inhibitors of Streptococcus pneumoniae surface endonuclease EndA discovered by high-throughput screening using a PicoGreen fluorescence assay

Journal of Biomolecular Screening

Volumn 18, Issue 3, 2013, Pages 247-257

  Peterson, Eliza J R ^a   Kireev, Dmitri ^b   Moon, Andrea F ^c   Midon, Marika ^d   Janzen, William P ^b   Pingoud, Alfred ^d   Pedersen, Lars C ^c   Singleton, Scott F ^a,b  

^a UNIVERSITY OF NORTH CAROLINA SCHOOL OF MEDICINE   (United States)

^b University of North Carolina   (United States)

^c NATIONAL INSTITUTE OF ENVIRONMENTAL HEALTH SCIENCES   (United States)

^d JUSTUS LIEBIG UNIVERSITY   (Germany)

Author keywords

EndA; endonuclease; high throughput screening (HTS); PicoGreen; Streptococcus pneumoniae; virulence factor

Indexed keywords

DNA; DOUBLE STRANDED DNA; ENDONUCLEASE; ENDONUCLEASE A; FLUORESCENT DYE; UNCLASSIFIED DRUG; VIRULENCE FACTOR;

ARTICLE; CONTROLLED STUDY; ENZYME ACTIVITY; GENE TRANSFER; GENETIC VARIABILITY; HIGH THROUGHPUT SCREENING; HYDROLYSIS; IMMUNITY; NONHUMAN; PNEUMOCOCCAL INFECTION; PRIORITY JOURNAL; STREPTOCOCCUS PNEUMONIAE;

BACTERIAL PROTEINS; DNA; ENDODEOXYRIBONUCLEASES; ENZYME INHIBITORS; FLUORESCENCE; HIGH-THROUGHPUT SCREENING ASSAYS; MEMBRANE PROTEINS; MICROCOCCAL NUCLEASE; ORGANIC CHEMICALS; REPRODUCIBILITY OF RESULTS; STREPTOCOCCUS PNEUMONIAE; VIRULENCE FACTORS;

EID: 84873622537     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057112461153     Document Type: Article

References (34)

1. Van der Poll T., Opal S. M.. Pathogenesis, treatment, and prevention of pneumococcal pneumonia. Lancet. 2009 ; 374: 1543-1556
2. Paterson G. K., Orihuela C. J.. Pneumococci: Immunology of the Innate Host Response. Respirology. 2010 ; 15: 1057-1063
3. Jonsson S., Musher D. M., Chapman A., Goree A., Lawrence E. C.. hagocytosis and Killing of Common Bacterial Pathogens of the Lung by Human Alveolar Macrophages. J. Infect. Dis. 1985 ; 152: 4-13 (Pubitemid 15038828)
4. Mitchell A. M., Mitchell T. J.. Streptococcus pneumoniae: Virulence Factors and Variation. Clin. Microbiol. Infect. 2010 ; 16: 411-418
5. Lacks S.. Mutants of Diplococcus pneumoniae that Lack Deoxyribonucleases and Other Activities Possibly Pertinent to Genetic Transformation. J. Bacteriol. 1970 ; 101: 373-383
6. Lacks S., Greenberg B., Neuberger M.. Role of a Deoxyribonuclease in Genetic Transformation of Diplococcus pneumoniae. Proc. Natl. Acad. Sci. U. S. A. 1974 ; 71: 2305-2309
7. Lacks S., Greenberg B., Neuberger M.. Identification of a Deoxyribonuclease Implicated in Genetic Transformation of Diplococcus pneumoniae. J. Bacteriol. 1975 ; 123: 222-232
8. Puyet A., Greenberg B., Lacks S. A.. Genetic and Structural Characterization of endA: A Membrane-Bound Nuclease Required for Transformation of Streptococcus pneumoniae. J. Mol. Biol. 1990 ; 213: 727-738 (Pubitemid 20213212)
9. Claverys J., Martin B., Polard P.. The Genetic Transformation Machinery: Composition, Localization, and Mechanism. FEMS Microbiol. Rev. 2009 ; 33: 643-656
10. Hiller N. L., Janto B., Hogg J. S., Boissy R., Yu S., Powell E., Keefe R., Ehrlich N. E., Shen K., Hayes J., Barbadora K., Klimke W., Dernovoy D., Tatusova T., Parkhill J., Bentley S. D., Post J. C., Ehrlich G. D., Hu F. Z.. Comparative Genomic Analyses of Seventeen Streptococcus pneumoniae Strains: Insights into the Pneumococcal Supragenome. J. Bacteriol. 2007 ; 189: 8186-8195 (Pubitemid 350178930)
11. Ehrlich G. D., Hiller N. L., Hu F. Z.. What Makes Pathogens Pathogenic. Genome Biol. 2008 ; 9: 225
12. Brinkmann V., Reichard U., Goosmann C., Fauler B., Uhlemann Y., Weiss D. S., Weinrauch Y., Zychlinsky A.. Neutrophil Extracellular Traps Kill Bacteria. Science. 2004 ; 303: 1532-1535 (Pubitemid 38314426)
13. Von Kockritz-Blickwede M., Nizet V.. Innate Immunity Turned inside-out: Antimicrobial Defense by Phagocyte Extracellular Traps. J. Mol. Med. 2009 ; 87: 775-783
14. Wartha F., Beiter K., Albiger B., Fernebro J., Zychlinsky A., Normark S., Henriques-Normark B.. Capsule and D- Alanylated Lipoteichoic Acids Protect Streptococcus pneumoniae against Neutrophil Extracellular Traps. Cell. Microbiol. 2007 ; 9: 1162-1171 (Pubitemid 46547136)
15. Beiter K., Wartha F., Albiger B., Normark S., Zychlinsky A., Henriques-Normark B.. An Endonuclease Allows Streptococcus pneumoniae to Escape from Neutrophil Extracellular Traps. Curr. Biol. 2006 ; 16: 401-407 (Pubitemid 43259351)
16. Tolun G., Myers R. S.. A Real-Time DNase Assay (ReDA) Based on PicoGreen Fluorescence. Nucleic Acids Res. 2003 ; 31: e111
17. Moon A. F., Midon M., Meiss G., Pingoud A., London R. E., Pedersen L. C.. Structural Insights into Catalytic and Substrate Binding Mechanisms of the Strategic EndA Nuclease from Streptococcus pneumoniae. Nucleic Acids Res. 2011 ; 39: 2943-2953
18. Lipinski C. A.. Lead- and Drug-like Compounds: The Rule-of-Five Revolution. Drug Discovery Today. 2004 ; 1: 337-341 (Pubitemid 40186335)
19. Zhang Y., Wang Y., Liu B.. Peptide-Mediated Energy Transfer between an Anionic Water-Soluble Conjugated Polymer and Texas Red Labeled DNA for Protease and Nuclease Activity Study. Anal. Chem. 2009 ; 81: 3731-3737
20. Xu X., Han M. S., Mirkin C. A.. A Gold-Nanoparticle-Based Real-Time Colorimetric Screening Method for Endonuclease Activity and Inhibition. Angew. Chem. Int. Ed Engl. 2007 ; 46: 3468-3470 (Pubitemid 46973432)
21. Dragan A. I., Casas-Finet J. R., Bishop E. S., Strouse R. J., Schenerman M. A., Geddes C. D.. Characterization of PicoGreen Interaction with dsDNA and the Origin of Its Fluorescence Enhancement upon Binding. Biophys. J. 2010 ; 99: 3010-3019
22. Midon M., Schaefer P., Pingoud A., Ghosh M., Moon A. F., Cuneo M. J., London R. E., Meiss G.. Mutational and Biochemical Analysis of the DNA-Entry Nuclease EndA from Streptococcus pneumoniae. Nucleic Acids Res. 2011 ; 39: 623-634
23. Midon M., Gimadutdinow O., Meiss G., Friedhoff P., Pingoud A.. Chemical Rescue of Active Site Mutants of S. pneumoniae Surface Endonuclease EndA and Other Nucleases of the HNH Family by Imidazole. Chembiochem. 2012 ; 13: 713-721
24. Yokochi T., Robertson K. D.. Dimethyl Sulfoxide Stimulates the Catalytic Activity of De Novo DNA Methyltransferase 3a (Dnmt3a) In Vitro. Bioorg. Chem. 2004 ; 32: 234-243 (Pubitemid 38798266)
25. Feng B. Y., Shoichet B. K.. A Detergent-Based Assay for the Detection of Promiscuous Inhibitors. Nature Protocols. 2006 ; 1: 550-553
26. Zhang J. H., Chung T. D., Oldenburg K. R.. A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. J. Biomol. Screen. 1999 ; 4: 67-73 (Pubitemid 29278954)
27. Wang Y., Xiao J., Suzek T. O., Zhang J., Wang J., Zhou Z., Han L., Karapetyan K., Dracheva S., Shoemaker B. A., Bolton E., Gindulyte A., Bryant S. H.. PubChem's BioAssay Database. Nucleic Acids Res. 2012 ; 40: D400 - D412
28. McGovern S. L., Caselli E., Grigorieff N., Shoichet B. K.. A Common Mechanism Underlying Promiscuous Inhibitors from Virtual and High-Throughput Screening. J. Med. Chem. 2002 ; 45: 1712-1722 (Pubitemid 34293537)
29. Peterson E. J., Janzen W. P., Kireev D., Singleton S. F.. High-Throughput Screening for RecA Inhibitors Using a Transcreener Adenosine 5′-o-Diphosphate Assay. Assay Drug Dev. Technol. 2012 ; 10: 260-268
30. Perola E.. Minimizing False Positives in Kinase Virtual Screens. Proteins. 2006 ; 64: 422-435 (Pubitemid 43993650)
31. Baell J. B., Holloway G. A.. New Substructure Filters for Removal of Pan Assay Interference Compounds (PAINS) from Screening Libraries and for Their Exclusion in Bioassays. J. Med. Chem. 2010 ; 53: 2719-2740
32. Jadhav A., Ferreira R. S., Klumpp C., Mott B. T., Austin C. P., Inglese J., Thomas C. J., Maloney D. J., Shoichet B. K., Simeonov A.. Quantitative Analyses of Aggregation, Autofluorescence, and Reactivity Artifacts in a Screen for Inhibitors of a Thiol Protease. J. Med. Chem. 2010 ; 53: 37-51
33. Buchanan J. T., Simpson A. J., Aziz R. K., Liu G. Y., Kristian S. A., Kotb M., Feramisco J., Nizet V.. DNase Expression Allows the Pathogen Group A Streptococcus to Escape Killing in Neutrophil Extracellular Traps. Curr. Biol. 2006 ; 16: 396-400 (Pubitemid 43259350)
34. Berends E. T., Horswill A. R., Haste N. M., Monestier M., Nizet V., von Kockritz-Blickwede M.. Nuclease Expression by Staphylococcus aureus Facilitates Escape from Neutrophil Extracellular Traps. J. Innate Immun. 2010 ; 2: 576-586