Evaluation of carrier-mediated siRNA delivery: Lessons for the design of a stem-loop qPCR-based approach for quantification of intracellular full-length siRNA

Journal of Controlled Release

Volumn 166, Issue 3, 2013, Pages 220-226

  Colombo, Stefano ^a   Nielsen, Hanne Mørck ^a   Foged, Camilla ^a  

^a UNIVERSITY OF COPENHAGEN   (Denmark)

Author keywords

Drug delivery; Nanomedicine; qPCR; siRNA; Stem loop qPCR

Indexed keywords

ANALYTICAL CHALLENGE; ANALYTICAL PROTOCOLS; CARRIER-MEDIATED; DELIVERY VEHICLE; ENHANCED GREEN FLUORESCENT PROTEIN; IN-VITRO; ITS APPLICATIONS; QPCR; QUANTITATIVE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA INTERFERENCE; SIRNA; SIRNA DELIVERY; SMALL INTERFERING RNA (SIRNA); STEM-LOOP;

CELL CULTURE; DRUG DELIVERY; MEDICAL NANOTECHNOLOGY; POLYMERASE CHAIN REACTION;

RNA;

DOUBLE STRANDED RNA; ENHANCED GREEN FLUORESCENT PROTEIN; PRIMER RNA; SMALL INTERFERING RNA; SMALL NUCLEOLAR RNA;

ARTICLE; CELL MEMBRANE; CLINICAL PROTOCOL; CONTROLLED STUDY; DIMERIZATION; DRUG DELIVERY SYSTEM; ELECTROSPRAY MASS SPECTROMETRY; FLOW CYTOMETRY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; HYDROPHOBICITY; IN VITRO STUDY; LUNG NON SMALL CELL CANCER; NUCLEOTIDE SEQUENCE; PRACTICE GUIDELINE; PRIORITY JOURNAL; PROCESS OPTIMIZATION; PROTEIN EXPRESSION; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA FOLDING; RNA INTERFERENCE; RNA ISOLATION; SURFACE CHARGE; VALIDITY;

CELL CULTURE TECHNIQUES; CELL LINE, TUMOR; DRUG CARRIERS; FLOW CYTOMETRY; GENE SILENCING; GREEN FLUORESCENT PROTEINS; HUMANS; LIPOSOMES; LUCIFERASES, FIREFLY; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 84873446865     PISSN: 01683659     EISSN: 18734995     Source Type: Journal    
DOI: 10.1016/j.jconrel.2013.01.006     Document Type: Article

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