Quantitative three-dimensional analysis of the intracellular trafficking of plasmid DNA transfected by a nonviral gene delivery system using confocal laser scanning microscopy

Molecular Therapy

Volumn 9, Issue 3, 2004, Pages 443-451

  Akita, H ^a,b   Ito, R ^a,b   Khalil, I A ^a,b   Futaki, S ^c   Harashima, H ^a,b  

^a HOKKAIDO UNIVERSITY   (Japan)

^b JAPAN SCIENCE AND TECHNOLOGY AGENCY   (Japan)

^c KYOTO UNIVERSITY   (Japan)

Author keywords

Gene delivery; Intracellular pharmacokinetics; Nonviral; Quantification; Three dimensional

Indexed keywords

4 [5 (4 METHYL 1 PIPERAZINYL)[2,5' BI 1H BENZIMIDAZOL] 2' YL]PHENOL; ARGININE DERIVATIVE; DYE; LIPOFECTAMINE; PLASMID DNA; RHODAMINE; STEARIC ACID;

ARTICLE; CELL LABELING; CELL NUCLEUS; CELL TRANSPORT; CONTROLLED STUDY; CYTOPLASM; DNA DETERMINATION; ENDOSOME; GENE DELIVERY SYSTEM; GENE EXPRESSION; GENE TARGETING; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; IMAGE ANALYSIS; LYSOSOME; NONVIRAL GENE DELIVERY SYSTEM; PROCESS OPTIMIZATION; QUANTITATIVE ANALYSIS; THREE DIMENSIONAL IMAGING; TRANSGENE;

EID: 1642443376     PISSN: 15250016     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.ymthe.2004.01.005     Document Type: Article

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