Heparinase treatment of RNA before quantitative real-time RT-PCR

BioTechniques

Volumn 35, Issue 6, 2003, Pages 1140-1144

  Johnson, Mary Lynn ^a   Navanukraw, Chainarong ^a   Grazul Bilska, Anna T ^a   Reynolds, Lawrence P ^a   Redmer, Dale A ^a  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ENZYME INHIBITOR; HEPARIN LYASE; RIBONUCLEASE; RIBONUCLEASE INHIBITOR; RNA; RNASIN; UNCLASSIFIED DRUG;

ANALYTIC METHOD; ARTICLE; DIAGNOSTIC KIT; MOLONEY LEUKEMIA ONCOVIRUS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ISOLATION;

MOLONEY MURINE LEUKEMIA VIRUS; ONCOVIRINAE;

EID: 0344737838     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/03356bm03     Document Type: Article

References (6)

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2. Izraeli, S., C. Pfleiderer, and T. Lion. 1991. Detection of gene expression by PCR amplification of RNA derived from frozen heparinized whole blood. Nucleic Acids Res. 19:6051.
3. Imai, H., O. Yamada, S. Morita, S. Suehiro, and T. Kurimura. 1992. Detection of HIV-1 RNA in heparinized plasma of HIV-1 seropositive individuals. J. Virol. Methods 36:181-184.
4. Jung, R., C. Lubcke, C. Wagener, and M. Neumaier. 1997. Reversal of RT-PCR inhibition observed in heparinized clinical specimens. BioTechniques 23:24-28.
5. Beutler, E., T. Gelbart, and W. Kuhl. 1990. Interference of heparin with the polymerase chain reaction. BioTechniques 9:166.
6. Bai, X., S. Fischer, S. Keshavjee, and M. Liu. 2000. Heparin interference with reverse transcriptase polymerase chain reaction of RNA extracted from lungs after ischemia-reperfusion. Transpl. Int. 10:146-150.