Estimation of the deamidation rates of major deamidation sites in a fab fragment of mouse IgG1-κ by capillary isoelectric focusing of mutated fab fragments

Analytical Chemistry

Volumn 85, Issue 3, 2013, Pages 1705-1710

  Shimura, Kiyohito ^a   Hoshino, Makoto ^b   Kamiya, Keiichiro ^b   Enomoto, Manabu ^b   Hisada, Sunao ^c   Matsumoto, Hiroyuki ^c   Novotny, Mark ^d   Kasai, Ken Ichi ^b  

^a FUKUSHIMA MEDICAL UNIVERSITY   (Japan)

^b TEIKYO UNIVERSITY   (Japan)

^c CENTRAL RESEARCH LABORATORY   (Japan)

^d J CRAIG VENTER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIGEN BINDING; ASN RESIDUES; CAPILLARY ISOELECTRIC FOCUSING; DEAMIDATION; FAB FRAGMENTS; PEPTIDE FRAGMENTS; PEPTIDE MAPPING; PHOSPHATE BUFFERS; QUANTITATIVE METHOD; SITE DIRECTED MUTAGENESIS; TRYPTIC PEPTIDES;

AMIDES; AMINO ACIDS; ELECTROPHORESIS; MAMMALS; MAPPING; MASS SPECTROMETRY; NUMBERING SYSTEMS; PEPTIDES; RATE CONSTANTS; RECOMBINANT PROTEINS;

CHEMICAL REACTIONS;

IMMUNOGLOBULIN F(AB) FRAGMENT; IMMUNOGLOBULIN KAPPA CHAIN;

ANIMAL; ARTICLE; CAPILLARY ELECTROPHORESIS; GENETICS; ISOELECTRIC FOCUSING; METABOLISM; METHODOLOGY; MOUSE; MUTATION; PHYSIOLOGY;

ANIMALS; ELECTROPHORESIS, CAPILLARY; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN KAPPA-CHAINS; ISOELECTRIC FOCUSING; MICE; MUTATION;

EID: 84873332443     PISSN: 00032700     EISSN: 15206882     Source Type: Journal    
DOI: 10.1021/ac3033992     Document Type: Article

References (15)

1. Robinson, N. E. and Robinson, A. B. Molecular Clocks: Deamidation of Asparaginyl and Glutaminyl Residues in Peptides and Proteins; Althouse Press: Cave Junction, OR, 2004.
2. Aswad, D. W. Ann. N.Y. Acad. Sci. 1990, 613, 26-36
3. Gaza-Bulseco, G.; Li, B.; Bulseco, A.; Liu, H. C. Anal. Chem. 2008, 80, 9491-9498
4. Mimura, Y.; Nakamura, K.; Tanaka, T.; Fujimoto, M. Electrophoresis 1998, 19, 767-775
5. Perkins, M.; Theiler, R.; Lunte, S.; Jeschke, M. Pharm. Res. 2000, 17, 1110-1117
6. Kroon, D. J.; Baldwin-Ferro, A.; Lalan, P. Pharm. Res. 1992, 9, 1386-1393
7. Shimura, K.; Hoshino, M.; Kamiya, K.; Katoh, K.; Hisada, S.; Matsumoto, H.; Kasai, K. Electrophoresis 2002, 23, 909-917
8. Krebber, A.; Bornhauser, S.; Burmester, J.; Honegger, A.; Willuda, J.; Bosshard, H. R.; Pluckthun, A. J. Immunol. Methods 1997, 201, 35-55
9. Ho, S. N.; Hunt, H. D.; Horton, R. M.; Pullen, J. K.; Pease, L. R. Gene 1989, 77, 51-59
10. Wan, H.; Ohman, M.; Blomberg, L. G. J. Chromatogr. A 2001, 924, 59-70.
11. Robinson, N. E.; Robinson, A. B. Proc. Natl. Acad. Sci. U.S.A. 2001, 98, 944-949
12. Shimura, K.; Kamiya, K.; Matsumoto, H.; Kasai, K. Anal. Chem. 2002, 74, 1046-1053
13. Mack, S.; Cruzado-Park, I.; Chapman, J.; Ratnayake, C.; Vigh, G. Electrophoresis 2009, 30, 4049-4058
14. Robinson, N. E.; Robinson, A. B. In Molecular Clocks: Deamidation of Asparaginyl and Glutaminyl Residues in Peptides and Proteins; Althouse Press: Cave Junction, OR, 2004, pp 82-83.
15. Robinson, N. E.; Robinson, A. B. In Molecular Clocks: Deamidation of Asparaginyl and Glutaminyl Residues in Peptides and Proteins; Althouse Press: Cave Junction, OR, 2004, pp 103-110.