A systematic study in CIEF: Defining and optimizing experimental parameters critical to method reproducibility and robustness

Electrophoresis

Volumn 30, Issue 23, 2009, Pages 4049-4058

  Mack, Scott ^a   Cruzado Park, Ingrid ^a   Chapman, Jeff ^a   Ratnayake, Chitra ^a   Vigh, Gyula ^b  

^a BECKMAN COULTER INC   (United States)

^b TEXAS A AND M UNIVERSITY   (United States)

Author keywords

CE; IEF; mAbs; Therapeutic protein characterization

Indexed keywords

AMINO ACIDS; PROTEINS; SODIUM HYDROXIDE;

AMPHOLYTES; CARRIER AMPHOLYTES; CE; IEF; ISOFORMS; MAB; PROTEIN CHARACTERIZATION; SYSTEMATIC STUDY; THERAPEUTIC PROTEIN; THERAPEUTIC PROTEIN CHARACTERIZATION;

UREA;

DIGLYCINE; MONOCLONAL ANTIBODY; PHOSPHORIC ACID; SODIUM HYDROXIDE;

ACCURACY; ANALYTIC METHOD; CONFERENCE PAPER; ELECTROSPRAY MASS SPECTROMETRY; FULL COLUMN IMAGING CIEF ANALYSIS; MOBILIZATION; PH; PRECIPITATION; PROCESS OPTIMIZATION; PROTEIN AGGREGATION; PROTEIN ANALYSIS; REPRODUCIBILITY; SEPARATION TECHNIQUE; SOLUBILITY;

EID: 72849122502     PISSN: 01730835     EISSN: 15222683     Source Type: Journal    
DOI: 10.1002/elps.200800690     Document Type: Conference Paper

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