Lipase applications in oil hydrolysis with a case study on castor oil: A review

Critical Reviews in Biotechnology

Volumn 33, Issue 1, 2013, Pages 81-96

  Goswami, Debajyoti ^a   Basu, Jayanta Kumar ^a   De, Sirshendu ^a  

^a INDIAN INSTITUTE OF TECHNOLOGY   (India)

Author keywords

Castor oil; Hydrolysis; Lipase; Ricinoleic acid; Surfactant; Systematic optimization

Indexed keywords

ALCOHOLYSIS; BUFFER CONCENTRATIONS; CASTOR OIL; DEGREE OF HYDROLYSIS; DIFFERENT EFFECTS; ENZYME CONCENTRATIONS; ENZYME LOADING; ESTOLIDES; HYDROPHILIC SOLVENTS; LIPASE-CATALYZED; LIPOLASE; MEMBRANE REACTOR; OPERATING RANGES; OPTIMIZATION METHOD; OPTIMUM CONDITIONS; PROCESS VARIABLES; REVERSE MICELLES; RICINOLEIC ACID; SINGLE VARIABLE; SPAN 80; STATISTICAL OPTIMIZATION; SYSTEMATIC OPTIMIZATION; THERMAL INACTIVATION; TRIACYLGLYCEROLS;

ANIONIC SURFACTANTS; BIOREACTORS; CATALYSIS; GLYCEROL; HYDROLYSIS; METAL IONS; MONOUNSATURATED FATTY ACIDS; NONIONIC SURFACTANTS; OPTIMIZATION; ORGANIC SOLVENTS; SURFACE ACTIVE AGENTS; VEGETABLE OILS;

LIPASES;

ANION; BUFFER; CASTOR OIL; CATION; LINOLEIC ACID; METAL ION; NONIONIC SURFACTANT; ORGANIC SOLVENT; REAGENT; RICINOLEIC ACID; SORBITAN OLEATE; TRIACYLGLYCEROL LIPASE; TWITCHELL REAGENT; UNCLASSIFIED DRUG;

ASPERGILLUS NIGER; ASPERGILLUS ORYZAE; BACTERIUM; BIOCATALYSIS; CANDIDA; CATALYSIS; CHEMICAL STRUCTURE; ENZYME IMMOBILIZATION; FUNGUS; GALACTOMYCES GEOTRICHUM; HUMICOLA LANUGINOSE; HYDROPHILICITY; HYDROPHOBICITY; LACTOBACILLUS PLANTARUM; LIPID HYDROLYSIS; MEMBRANE REACTOR; MICELLE; NONHUMAN; PENICILLIUM; PHYSICAL CHEMISTRY; PLANT; PRESSURE; PRIORITY JOURNAL; PSEUDOMONAS; PSEUDOMONAS FLUORESCENS; PSEUDOMONAS GESSARDII; REVIEW; RHIZOPUS; TEMPERATURE; THERMAL ANALYSIS; THERMOMYCES LANUGINOSUS; ULTRASOUND;

BIOREACTORS; BIOTECHNOLOGY; CASTOR OIL; HYDROLYSIS; LIPASE; SURFACE-ACTIVE AGENTS;

EID: 84873172820     PISSN: 07388551     EISSN: 15497801     Source Type: Journal    
DOI: 10.3109/07388551.2012.672319     Document Type: Review

References (115)

1. Abdou AM. 2003. Purification and partial characterization of psychrotrophic Serratia marcescens lipase. J Dairy Sci 86: 127-132. (Pubitemid 39067166)
2. Adhvaryu A, Sharma BK, Erhan SZ. 2005. Current developments of biodegradable grease. In: Erhan SZ, ed. Industrial Uss of Vegetable Oils. Champaign: AOCS Press, 14-30.
3. Akoh CC, Chang SW, Lee GC, Shaw JF. 2007. Enzymatic approach to biodiesel production. J Agric Food Chem 55: 8995-9005. (Pubitemid 350105547)
4. Al-Zuhair S, Hasan M, Ramachandran KB. 2003. Kinetics of the enzymatic hydrolysis of palm oil by lipase. Process Biochem 38: 1155-1163. (Pubitemid 36639644)
5. Barnes JF. 1992. Calcium sulfonate grease and method of manufacture. US Patent 5126062.
6. Bednarcyk NE, Erickson WL. 1973. Fatty acid: Synthesis and applications. New Jersey: Noyes Data Corporation.
7. Benjamin S, Pandey A. 1998. Candida rugosa lipases: Molecular biology and versatility in biotechnology. Yeast 14: 1069-1087. (Pubitemid 28412712)
8. Bhardwaj K, Raju A, Rajasekharan R. 2001. Identification, purification, and characterization of a thermally stable lipase from rice bran. A new member of the (phospho) lipase family. Plant Physiol 127: 1728-1738. (Pubitemid 34054213)
9. Braga A, Gomes N, Teixeira JA, Belo I. 2009. Effect of castor oil hydrolysis in the production of γdecalactone by the yeast Yarrowia lipolytica. In: Book of Abstracts of Microbiotec09 Meeting. Portugal: Departamento de Engenharia Biológica, Universidade do Minho 245.
10. Carvalho CM, Cabral JM. 2000. Reverse micelles as reaction media for lipases. Biochimie 82: 1063-1085.
11. de Sousa JS, Cavalcanti-Oliveira ED, Aranda DAG, Freire DMG. 2010. Application of lipase from the physic nut (Jatropha curcas L.) to a new hybrid (enzyme/chemical) hydroesterification process for biodiesel production. J Mol Catal B: Enzym 65: 133-137.
12. Deǧerli N, Akpinar MA. 2002. Partial purification of intestinal triglyceride lipase from cyprinion macrostomus heckle, 1843 and effect of pH on enzyme activity. Turk J Biol 26: 133-143.
13. Demir BS, Tükel SS. 2010. Purification and characterization of lipase from Spirulina platensis. J Mol Catal B: Enzym 64: 123-128.
14. Deng HT, Xu ZK, Dai ZW, Wu J, Seta P. 2005. Immobilization of Candida rugosa lipase on polypropylene microfiltration membrane modified by glycopolymer: Hydrolysis of olive oil in biphasic bioreactor. Enzyme Microb Technol 36: 996-1002. (Pubitemid 40598036)
15. Dheeman DS, Antony-Babu S, Frias JM, Henehan GTM. 2011. Purification and characterization of an extracellular lipase from a novel strain Penicillium sp. DS-39 (DSM 23773). J Mol Catal B: Enzym 72: 256-262.
16. Diamond MJ, Neddles HL, Elliger CA, Kertesz DJ, Frankel EN, Fuller G. 1971. Hydroxyaliphatic acids in branched copolymers with acyl chloride-terminated long side chains: A new shrink-resist treatment for wool. J Am Oil Chem Soc 48: 678-681.
17. Edwinoliver NG, Thirunavukarasu K, Purushothaman S, Rose C, Gowthaman MK, Kamini NR. 2009. Corn steep liquor as a nutrition adjunct for the production of Aspergillus niger lipase and hydrolysis of oils thereof. J Agric Food Chem 57: 10658-10663.
18. Er-Zheng S, Ying Z, Peng-Yong Y, Dong-Zhi W. 2010. Lipases in the castor bean seed of Chinese varieties: Activity comparison, purification and characterization. J Shanghai Univ (Engl Ed) 14: 137-144.
19. Foglia TA, Zones KC, Sonnet PE. 2000. Selectivity of lipases: Isolation of fatty acids from castor, coriander, and meadowfoam oils. Eur J Lipid Sci Technol 102: 612-617.
20. Gaur R, Khare SK. 2011. Statistical optimization of palm oil hydrolysis by Pseudomonas aeruginosa PseA lipase. Asia-Pac J Chem Eng 6: 147-153.
21. Ghosh M, Bhattacharyya DK. 1998. Enzymatic preparation of polyethylene glycol esters of castor oil fatty acids and their surface-active properties. J Surfactants Detergents 1: 503-505. (Pubitemid 128544904)
22. Glogauer A, Martini VP, Faoro H, Couto GH, Müller-Santos M, Monteiro RA, Mitchell DA, de Souza EM, Pedrosa FO, Krieger N. 2011. Identification and characterization of a new true lipase isolated through metagenomic approach. Microb Cell Fact 10: 54.
23. Goswami D, Basu JK, De S. 2009a. Optimization of process variables in castor oil hydrolysis by Candida rugosa lipase with buffer as dispersion medium. Biotechnol Bioprocess Eng 14: 220-224.
24. Goswami D, Sen R, Basu JK, De S. 2009b. Maximization of bioconversion of castor oil into ricinoleic acid by response surface methodology. Bioresour Technol 100: 4067-4073.
25. Goswami D, Sen R, Basu JK, De S. 2010. Surfactant enhanced ricinoleic acid production using Candida rugosa lipase. Bioresour Technol 101: 6-13.
26. Goto M, Hatanaka C, Haraguchi T. 2000a. Hydrolysis of castor oil by lipase immobilized on hollow fiber membrane bioreactor. Research Report of Kitakyushu Technical College 33: 151-155.
27. Goto M, Hatanaka C, Haraguchi T. 2000b. Selective production of ricinoleic acid by hydrolysis of castor oil using lipase immobilized in N-polyisopropylacrylamide gel. Kagaku Kogaku Ronbunshu 26: 402-405.
28. Gupta R, Gupta N, Rathi P. 2004. Bacterial lipases: An overview of production, purification and biochemical properties. Appl Microbiol Biotechnol 64: 763-781. (Pubitemid 38918139)
29. Gupta S, Ingole P, Singh K, Bhattacharya A. 2011. Comparative study of the hydrolysis of different oils by lipase-immobilized membranes. J Appl Polym Sci. DOI: 10.1002/app.35400.
30. Habulin M, Knez Z. 2002. High-pressure enzymatic hydrolysis0 of oil. Eur J Lipid Sci Technol 104: 381-386.
31. Hochkoeppler A, Palmieri S. 1990. A spectrophotometric lipase activity assay by coupled lipase-oxygenase in reversed micelles. Biocatal Biotransform 3: 357-365.
32. Ibrahim CO, Hayashi M, Nagai S. 1987. Purification and some properties of a thermostable lipase from Humicola lanuginosa No. 3. Agric Biol Chem 51: 37-45.
33. Ishchuk YL, Dugina LN, Krasnokutskaya ME, Godun BA. 1986. Influence of composition of technical 12-hydroxystearic acid on properties of anhydrous calcium greases. Chem Technol Fuels Oils 22: 402-405. (Pubitemid 17623045)
34. Ishchuk YL, Ishchuk LP, Maskaev AK, Kotok AA. 1974. Influence of adding oleic and ricinoleic acids to 12-hydroxystearic acid on the structure and properties of lithium greases. Chem Technol Fuels Oils 10: 833-835.
35. Iwai M, Tsujisaka Y, Fukumoto J. 1964. Studies on lipase, II. Hydrolytic and esterifying actions of crystalline lipase of Aspergillus niger. J Gen Appl Microbiol 10: 13-22.
36. Iwamatsu H, Akiyama M, Miyamoto Y, Okamura S. 2008. Lithium grease composition sealed in a small motor bearing to reduce noise. US Patent Application 20080305973.
37. Jachmanián I, Mukherjee KD. 1995. Germinating rapeseed as biocatalyst: Hydrolysis of oils containing common and unusual fatty acids. J Agric Food Chem 43: 2997-3000.
38. Jaeger KE, Reetz MT. 1998. Microbial lipases form versatile tools for biotechnology. Trends Biotechnol 16: 396-403. (Pubitemid 28410428)
39. Jensen RG. 1974. Characteristics of the lipase from the mold, Geotrichum candidum: A review. Lipids 9: 149-157.
40. Jeon GJ, Yang JW, Hur BK. 1999. Hydrolysis of castor oil with lipases and organic solvents. Korean J Biotechnol Bioeng 14: 696-701.
41. Johnson, RW, Fritz E, ed. 1989. Fatty acids in industry: Processes, derivatives, applications. New York: Marcel Dekker Inc.
42. Kallyanpur MR, Subrahmanyam VVR, Kane JG. 1967a. Fat splitting. III. Twitchell splitting of castor oil and hydrogenated castor oil. Indian J Technol 5: 20-22.
43. Kallyanpur MR, Subrahmanyam VVR, Kane JG. 1967b. Studies in fat splitting. IV. High pressure splitting of hydrogenated castor oil. Indian J Technol 5: 63-65.
44. Kirk RE, Othmer DF. 2004. Kirk-Othmer encyclopedia of chemical technology. Vol. 5. New York: John Wiley and Sons.
45. Kishino S, Ogawa J, Ando A, Omura Y, Shimizu S. 2002. Ricinoleic acid and castor oil as substrates for conjugated linoleic acid production by washed cells of Lactobacillus plantarum. Biosci Biotechnol Biochem 66: 2283-2286. (Pubitemid 39251242)
46. Kishino S, Ogawa J, Yokozeki K, Shimizu S. 2009. Microbial production of conjugated fatty acids. Lipid Technol 21: 177-181.
47. Knezevic ZD, Siler-Marinkovic SS, Mojovic LV. 1998. Kinetics of lipasecatalyzed hydrolysis of palm oil in lecithin/izooctane reversed micelles. Appl Microbiol Biotechnol 49: 267-271. (Pubitemid 28172639)
48. Kosugi Y, Takahashi K, Lopez C. 1995. Large-scale immobilization of lipase from Pseudomonas fluorescens biotype I and an application for sardine oil hydrolysis. J Am Oil Chem Soc 72: 1281-1285.
49. Krishnamurti N. 1980. Vinyl monomers from 12-hydroxy stearic acid. Pigm Resin Technol 9: 15-17.
50. Kuchel PW. 1997. Schaum's outline of theory and problems of biochemistry. New York: McGraw-Hill Professional.
51. Kulkarni SR. 2001. Studies in Enzyme modification. Ph.D. Thesis. Mumbai: Mumbai University.
52. Kulkarni N. 2002. Studies of Lipase Enzyme from Pseudomonas fluorescens NS2W. Ph.D. Thesis. Pune: University of Pune.
53. Kulkarni MG, Sawant SB. 2003. Some physical properties of castor oil esters and hydrogenated castor oil esters. Eur J Lipid Sci Technol 105: 214-218. (Pubitemid 36623258)
54. Kulkarni SR, Pandit AB. 2005. Enzymatic hydrolysis of castor oil: An approach for rate enhancement and enzyme economy. Indian J Biotechnol 4: 241-245. (Pubitemid 40780292)
55. Kuo TM, Gardner HW, ed. 2002. Lipid biotechnology. New York: Marcel Dekker Inc.
56. Lakshminarayana G, Subbarao R, Sastry YSR, Rao TC, Kale V, Gangadhar A. 1984a. High pressure splitting of castor oil. J Am Oil Chem Soc 61: 1204-1206. (Pubitemid 14601408)
57. Lakshminarayana G, Paulose MM, Kumari BN. 1984b. Characteristics and composition of newer varieties of Indian castor seed and oil. J Am Oil Chem Soc 61: 1871-1872. (Pubitemid 15479637)
58. Lavayre J, Baratti J. 1982. Preparation and properties of immobilized lipases. Biotechnol Bioeng 24: 1007-1013.
59. Lee DW, Kim HW, Lee KW, Kim BC, Choe EA, Lee HS, Kim DS, Pyun YR. 2001. Purification and characterization of two distinct thermostable lipases from the gram-positive thermophilic bacterium Bacillus thermoleovorans ID-1. Enzyme Microb Technol 29: 363-371.
60. Li H, Zhang X. 2005. Characterization of thermostable lipase from thermophilic Geobacillus sp. TW1. Protein Expr Purif 42: 153-159. (Pubitemid 40793405)
61. Lindner RA, Dudek E. 1995. External lubricant and stabilizer compositions for rigid vinyl polymers. US Patent 5426144.
62. Lopez F, Cinelli G, Ambrosonea L, Colafemmina G, Ceglie A, Palazzo G. 2004. Role of the cosurfactant in water-in-oil microemulsion: Interfacial properties tune the enzymatic activity of lipase. Colloids Surf A 237: 49-59. (Pubitemid 38543233)
63. Macedo G, Lopes CB, Fleuri LF. 2009. Hydrolysis optimization of castor oil by lipozyme. New Biotechnol 25: S150.
64. Magne FC, Mod RR, Skau EL. 1961. Fatty acid morpholides as plasticizers for vinyl chloride resins. II. the morpholides of selectively hydrogenated and of epoxidized fatty acids. J Am Oil Chem Soc 38: 291-293.
65. Manoj KM, Swaminathan T. 1997. Ester hydrolyses in reversed micelles using lipase. Bioprocess Eng 17: 185-188.
66. Mukherjee KD, Kiewitt I. 1996. Enrichment of very long chain monounsaturated fatty acids by lipase-catalysed hydrolysis and transesterification. Appl Microbiol Biotechnol 44: 557-562. (Pubitemid 26053293)
67. Murty VR, Bhat J, Muniswaran PK. 2004. Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane. Biotechnol Lett 26: 563-567. (Pubitemid 38463724)
68. Ogunniyi DS. 2006. Castor oil: A vital industrial raw material. Bioresour Technol 97: 1086-1091.
69. Okumura S, Iwai M, Tsujisaka Y. 1983. Synthesis of estolides during hydrolysis of castor oil by Geotrichum candidum lipase. Yukagaku 32: 271-273.
70. Ory RL, St Angelo AJ, Altschul AM. 1960. Castor bean lipase: Action on its endogenous substrate. J Lipid Res 1: 208-213.
71. Ory RL, St Angelo AJ, Altschul AM. 1962. The acid lipase of castor bean. properties and substrate specificity. J Lipid Res 3: 99-105.
72. Ozcan HM, Sagiroglu A. 2009. Production of ricinoleic acid from castor oil by immobilised lipases. Prep Biochem Biotechnol 39: 170-182.
73. Padmini P, Rakshit SK, Baradarajan A. 1993. Lipase catalysed hydrolysis of ricebran oil by free and immobilised enzyme system in batch stirred reactor. Bioprocess Eng 9: 103-106. (Pubitemid 23156463)
74. Palomo JM, Fuentes M, Fernández-Lorente G, Mateo C, Guisan JM, Fernández-Lafuente R. 2003. General trend of lipase to selfassemble giving bimolecular aggregates greatly modifies the enzyme functionality. Biomacromolecules 4: 1-6. (Pubitemid 36575615)
75. Parmar S, Hammond EG. 1994. Hydrolysis of fats and oils with moist oat caryopses. J Am Oil Chem Soc 71: 881-886.
76. Piazza GJ, Farrell HM, Jr. 1991. Generation of ricinoleic acid from castor oil using the lipase from ground oat (Avena Sativa L.) seeds as a catalyst. Biotechnol Lett 13: 179-184.
77. Potumarthi R, Subhakar Ch, Pavani A, Jetty A. 2008. Evaluation of various parameters of calcium-alginate immobilization method for enhanced alkaline protease production by Bacillus licheniformis NCIM-2042 using statistical methods. Bioresour Technol 99: 1776-1786.
78. Pugazhenthi G, Kumar A. 2004. Enzyme membrane reactor for hydrolysis of olive oil using lipase immobilized on modified PMMA composite membrane. J Membr Sci 228: 187-197. (Pubitemid 38064371)
79. Puthli MS, Rathod VK, Pandit AB. 2006. Enzymatic hydrolysis of castor oil: Process intensification studies. Biochem Eng J 31: 31-41. (Pubitemid 44224171)
80. Queiroz JA, Tomaz CT, Cabral JM. 2001. Hydrophobic interaction chromatography of proteins. J Biotechnol 87: 143-159. (Pubitemid 32241097)
81. Ramani K, Boopathy R, Mandal AB, Sekaran G. 2011. Preparation of acidic lipase immobilized surface-modified mesoporous activated carbon catalyst and thereof for the hydrolysis of lipids. Catal Commun 14: 82-88.
82. Ramani K, Kennedy LJ, Ramakrishnan M, Sekaran G. 2010. Purification, characterization and application of acidic lipase from Pseudomonas gessardii using beef tallow as a substrate for fats and oil hydrolysis. Process Biochem 45: 1683-1691.
83. Rao KVSA, Paulose MM, Lakshminarayana G. 1990. In situ lipolysis of castor oil in homogenised castor seeds. Biotechnol Lett 12: 377-380. (Pubitemid 20329098)
84. Rathi P, Bradoo S, Saxena RK, Gupta R. 2000. A hyper-thermostable, alkaline lipase from Pseudomonas sp. with the property of thermal activation. Biotechnol Lett 22: 495-498. (Pubitemid 30210589)
85. Rathod VK, Pandit AB. 2009. Effect of various additives on enzymatic hydrolysis of castor oil. Biochem Eng J 47: 93-99.
86. Rathod VK, Pandit AB. 2010. Enzymatic hydrolysis of oil in a spray column. J Mol Catal B: Enzym 67: 1-9.
87. Reis P, Holmberg K, Miller R, Leser ME, Raab T, Watzke HJ. 2009. Lipase reaction at interfaces as self-limiting processes. Competes Rendus Chimie 12: 163-170.
88. Rios GM, Belleville MP, Paolucci D, Sanchez J. 2004. Progress in enzymatic membrane reactors-a review. J Membr Sci 242: 189-196. (Pubitemid 39440386)
89. Sarada R, Joseph R. 1992. Purification and properties of lipase from the anaerobe Propionibacterium acidipropionici. J Am Oil Chem Soc 69: 974-977.
90. Saxena RK, Sheoran A, Giri B, Davidson WS. 2003a. Purification strategies for microbial lipases. J Microbiol Methods 52: 1-18. (Pubitemid 35232751)
91. Saxena RK, Davidson WS, Sheoran A, Giri B. 2003b. Purification and characterization of an alkaline thermostable lipase from Aspergillus carneus. Process Biochem 39: 239-247. (Pubitemid 38033686)
92. Schäfer T, Kirk O, Borchert TV, Fuglsang CC, Pedersen S, Salmon S, Olsen HS, Deinhammer R, Lund H. 2005. Enzymes for technical applications. In: Fahnestock, SR, Steinbüchel A, eds. Biopolymers. Weinheim: Wiley VCH, 377-437.
93. Schwitzer MK. 1997. Recent developments in castor oil products. In: Mani VVS, Shitole AD, eds. Fats, Oleochemicals and Surfactants: Challenges in the 21st Century. New Delhi: Oxford and IBH Publishing Co. Pvt. Ltd., 113-117.
94. Shamel MM, Ramachandran KB, Hasan M, Al-Zuhair S. 2007. Hydrolysis of palm and olive oils by immobilized lipase using hollow-fibre reactor. Biochem Eng J 34: 228-235. (Pubitemid 46486717)
95. Sharma R, Chisti Y, Banerjee UC. 2001. Production, purification, characterization, and applications of lipases. Biotechnol Adv 19: 627-662. (Pubitemid 34212866)
96. Sharon C, Furugoh S, Yamakido T, Ogawa HI, Kato Y. 1998a. Purification and characterization of a lipase from Pseudomonas aeruginosa KKA-5 and its role in castor oil hydrolysis. J Ind Microbiol Biotechnol 20: 304-307. (Pubitemid 28369942)
97. Sharon C, Nakazato M, Ogawa HI, Kato Y. 1998b. Lipase-induced hydrolysis of castor oil: Effect of various metals. J Ind Microbiol Biotechnol 21: 292-295. (Pubitemid 29116754)
98. Shu CH, Xu CJ, Lin GC. 2006. Purification and partial characterization of a lipase from Antrodia cinnamomea. Process Biochem 41: 734-738.
99. Shu ZY, Yang JK, Yan YJ. 2007. [Purification and characterization of a lipase from Aspergillus niger F044]. Sheng Wu Gong Cheng Xue Bao 23: 96-100.
100. Shuler ML, Kargi F. 2001. Bioprocess engineering: Basic concepts. 2nd ed., New Jersey: Prentice Hall.
101. Syed MN, Iqbal S, Bano S, Khan AB, Ali-ul-Qader S, Azhar A. 2010. Purification and characterization of 60 kD lipase linked with chaperonin from Pseudomonas aeruginosa BN-1. Afr J Biotechnol 9: 7724-7732.
102. Tagiri M, Endo Y, Fujimoto K, Sujuki, T. 1992. Hydrolysis of triglycerides of branched chain fatty acids and castor oil derivatives by pancreatic lipase. Biosci Biotechnol Biochem 56: 1490-1491.
103. Tan T, Zhang M, Xu J, Zhang J. 2004. Optimization of culture conditions and properties of lipase from Penicillium camembertii Thom PG-3. Process Biochem 39: 1495-1502. (Pubitemid 38871479)
104. Teomim D, Nyska A, Domb AJ. 1999. Ricinoleic acid-based biopolymers. J Biomed Mater Res 45: 258-267. (Pubitemid 29165942)
105. Vakhlu J, Kour A. 2006. Yeast lipases: Enzyme purification, biochemical properties and gene cloning. Electron J Biotechnol 9: 69-85. (Pubitemid 43200928)
106. Villeneuve P, Muderhwa JM, Graille J, Haas MJ. 2000. Customizing lipases for biocatalysis: A survey of chemical, physical and molecular biological approaches. J Mol Catal B: Enzym 9: 113-148. (Pubitemid 30195728)
107. Walde P, Luisi PL. 1990. Detection of water in lipase catalyzed reactions in reverse micelles as studied by infrared spectroscopy. Biocatal Biotransform 3: 381-383.
108. Wilson R, Van Schie BJ, Howes D. 1998. Overview of the preparation, use and biological studies on polyglycerol polyricinoleate (PGPR). Food Chem Toxicol 36: 711-718. (Pubitemid 28408690)
109. Yamamoto K, Fujiwara N. 1988. Purification and some properties of a castor-oil-hydrolyzing lipase from Pseudomonas sp. Agric Biol Chem 52: 3015-3021.
110. Yamamoto K, Fujiwara N. 1995. The hydrolysis of castor oil using a lipase from Pseudomonas sp. f-B-24: Positional and substrate specificity of the enzyme and optimum reaction conditions. Biosci Biotechnol Biochem 59: 1262-1266.
111. Yamane T. 1987. Enzyme technology for the lipids industry: An engineering overview. J Am Oil Chem Soc 64: 1657-1662.
112. Yan J, Yang J, Xu L, Yan Y. 2007. Gene cloning, overexpression and characterization of a novel organic solvent tolerant and thermostable lipase from Galactomyces geotrichum Y05. J Mol Catal B: Enzym 49: 28-35. (Pubitemid 47633257)
113. Yao C, Tang S, Heb Z, Deng X. 2005. Kinetics of lipase-catalyzed hydrolysis of olive oil in AOT/isooctane reversed micelles. J Mol Catal B: Enzym 35: 108-112. (Pubitemid 41557490)
114. Yu M, Qin S, Tan T. 2007. Purification and characterization of the extracellular lipase Lip2 from Yarrowia lipolytica. Process Biochem 42: 384-391. (Pubitemid 46329543)
115. Zaman MM, Hayashi Y, Talukder MMR, Kawanishi T. 2006. Activity of acetone-treated Chromobacterium viscosum lipase in AOT reverse micelles in the presence of low molecular weight polyethylene glycol. Biochem Eng J 29: 46-54.