Complete mapping of a cystine knot and nested disulfides of recombinant human arylsulfatase a by multi-enzyme digestion and LC-MS analysis using CID and ETD

Journal of the American Society for Mass Spectrometry

Volumn 24, Issue 1, 2013, Pages 125-133

  Ni, Wenqin ^a   Lin, Melanie ^b   Salinas, Paul ^b   Savickas, Philip ^b   Wu, Shiaw Lin ^a   Karger, Barry L ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

^b Shire Human Genetic Therapies Inc   (United States)

Author keywords

Cystine knot; Electron transfer dissociation; ETD; Free cysteines; Multi enzymes; Nested disulfides

Indexed keywords

CYSTINE KNOT; ELECTRON TRANSFER DISSOCIATION; ETD; FREE CYSTEINES; MULTI-ENZYMES; NESTED DISULFIDES;

AMINO ACIDS; DISSOCIATION; ELECTRON TRANSITIONS; ENZYMES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES;

SULFUR COMPOUNDS;

ARYLSULFATASE; ASPARTIC ACID; CYSTEINE; CYSTINE; DISULFIDE; ENZYME; FORMYLGLYCINE; GLYCINE; LYSINE; PEPSIN A; PEPTIDE; PNGASE F; RECOMBINANT ENZYME; TRYPSIN; UNCLASSIFIED DRUG;

ACCURACY; AMINO ACID ANALYSIS; AMINO TERMINAL SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; COLLISIONALLY ACTIVATED DISSOCIATION; DISSOCIATION; DISULFIDE BOND; ELECTRON TRANSPORT; ENZYME ACTIVITY; ENZYME STABILITY; ENZYME STRUCTURE; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PH; PHYSICAL CHEMISTRY; PROTEIN BINDING;

AMINO ACID SEQUENCE; CEREBROSIDE-SULFATASE; CHROMATOGRAPHY, LIQUID; CYSTEINE; DISULFIDES; HUMANS; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; PEPTIDE FRAGMENTS; PEPTIDE HYDROLASES; PEPTIDE MAPPING; RECOMBINANT PROTEINS;

EID: 84873052574     PISSN: 10440305     EISSN: 18791123     Source Type: Journal    
DOI: 10.1007/s13361-012-0510-z     Document Type: Article

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