Identification of the unpaired cysteine status and complete mapping of the 17 disulfides of recombinant tissue plasminogen activator using LC-MS with electron transfer dissociation/collision induced dissociation

Analytical Chemistry

Volumn 82, Issue 12, 2010, Pages 5296-5303

  Wu, Shiaw Lin ^a   Jiang, Haitao ^a   Hancock, William S ^a   Karger, Barry L ^a  

^a NORTHEASTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COLLISION INDUCED DISSOCIATION; COMPLETE MAPPINGS; CYSTEINE RESIDUES; DISULFIDE BONDS; DISULFIDE LINKAGES; ELECTRON TRANSFER; ELECTRON-TRANSFER DISSOCIATION; ENZYMATIC DIGESTIONS; FRAGMENT SIZES; GLUTATHIONES; GLYCOSIDIC CLEAVAGES; INDUCED DISSOCIATION; MULTIFRAGMENTATION; O-LINKED; TISSUE PLASMINOGEN ACTIVATOR;

CHEMICAL ACTIVATION; ELECTRON TRANSITIONS; PEPTIDES;

DISSOCIATION;

CYSTEINE; DISULFIDE; RECOMBINANT PROTEIN; TISSUE PLASMINOGEN ACTIVATOR;

AMINO ACID SEQUENCE; ARTICLE; CHEMISTRY; GLYCOSYLATION; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHODOLOGY; MOLECULAR GENETICS; PROCEDURES;

AMINO ACID SEQUENCE; CHROMATOGRAPHY, LIQUID; CYSTEINE; DISULFIDES; GLYCOSYLATION; MASS SPECTROMETRY; MOLECULAR SEQUENCE DATA; RECOMBINANT PROTEINS; TISSUE PLASMINOGEN ACTIVATOR;

EID: 77953562774     PISSN: 00032700     EISSN: None     Source Type: Journal    
DOI: 10.1021/ac100766r     Document Type: Article

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