A novel methodology for assignment of disulfide bond pairings in proteins

Protein Science

Volumn 6, Issue 2, 1997, Pages 391-398

  Wu, Jiang ^a   Watson, J Throck ^a,b  

^a MICHIGAN STATE UNIVERSITY   (United States)

^b Michigan State University   (United States)

Author keywords

chemical cleavage; cyanylation; disulfide bonds; MALDI MS; partial reduction

Indexed keywords

1 CYANO 4 DIMETHYLAMINOPYRIDINIUM TETRAFLUOROBORATE; CITRIC ACID; TRIS(2 CARBOXYETHYL)PHOSPHINE; UNCLASSIFIED DRUG;

ARTICLE; BASE PAIRING; BINDING AFFINITY; CHEMICAL REACTION; DISULFIDE BOND; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; REACTION ANALYSIS;

EID: 0031050298     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1002/pro.5560060215     Document Type: Article

References (24)

1. Brew K, Castellino FJ, Vanaman TC, Hill RL. 1970. The complete amino acid sequence of bovine α-lactalbumin. J Biol Chem 245:4570-4582.
2. Degani Y, Patchomik A. 1974. Cyanylation of sulfhydryl groups by 2-nitro-5-thiocyanobenzoic acid. High-yield modification and cleavage of peptides at cysteine residues. Biochemistry 13:1-11.
3. Gray WR. 1993a. Disulfide structures of highly bridged peptides: A new strategy for analysis. Protein Sci 2:1732-1748.
4. Gray WR. 1993b. Echistatin disulfide bridges: Selective reduction and linkage assignment. Protein Sci 2:1749-1755.
5. Hirayama K, Akashi S. 1994. Assignment of disulfide bonds in proteins. In: Matsuo T, Caprioli RM, Gross ML, Seyama Y, eds. Biological mass spectrometry: Present and future. New York: Wiley. pp 299-312.
6. Jacobson GR, Schaffer MH, Stark GR, Vanaman TC. 1973. Specific chemical cleavage in high yield at the amino peptide bonds of cysteine and cystine residues. J Biol Chem 248:6583-6591.
7. Kuwajima K, Ikeguchi M, Sugawara T, Hiraoka Y. Sugai S. 1990. Kinetics of disulfide bond reduction in α-lactalbumin by dithiothreitol and molecular basis of superreactivity of the cys6-cys120 disulfide bond. Biochemistry 29:8240-8249.
8. Lu HS, Gracy RW. 1981. Specific cleavage of glucosephosphate isomerases at cysteinyl residues using 2-nitro-5-thiocyanobenzoic acid: Analysis of peptides eluted from polyacrylamide gels and localization of active site histidyl and lysyl residues. Arch Biochem Biophys 212:347-359.
9. Nakagawa S, Tamakashi Y, Hamana T, Kawase M, Taketomi S, Ishibashi Y, Nishimura O, Fukuda T. 1994a. Chemical cleavage of recombinant fusion proteins to yield peptide amides. J Am Chem Soc 116:5513-5514.
10. Nakagawa S, Tamakashi Y, Ishibashi Y, Kawase M, Taketomi S, Nishimura O, Fukuda T. 1994b. Production of human PTH (1-34) via a recombinant DNA technique. Biochem Biophys Res Commun 200:1735-1741
11. Papayannopoulos IA, Biemann K. 1992. Amino acid sequence of a protease inhibitor isolated from Sarcophaga bullata determined by mass spectrometry. Protein Sci 1:278-288.
12. Reeve JR, Pierce JG. 1981. Disulfide bonds of glycoprotein hormones. Their selective reduction in the beta subunits of bovine lutropin and thyrotropin. Int J Pept Protein Res 18:79.
13. Regnier FE, Gooding KM. 1980. High-performance liquid chromatography of proteins. Anal Biochem 103:1-25.
14. Rubinstein M. 1979. Preparative high-performance liquid partition chromatography of proteins. Anal Biochem 98:1-7.
15. Ryle AP, Sanger F. 1955. Disulphide interchange reactions. Biochem J 60:535-540.
16. Siuzdak G. 1994. The emergence of mass spectrometry in biomedical research. Proc Natl Acad Sci USA 91:11290-11297.
17. Smith DL, Zhou Z. 1990. Strategies for locating disulfide bonds in proteins. Methods Enzymol 193:374-389.
18. Smyth DG, Stein WH, Moore S. 1963. The sequence of amino acid residues in bovine pancreatic ribonuclease: Revisions and confirmations. J Biol Chem 238:227-234.
19. Stark GR. 1977. Cleavage at cysteine after cyanylation. Methods Enzymol 47:129-132.
20. Takeda K, Ogawa K, Ohara M, Hamada S, Moriyama Y. 1995. Conformational changes of α-lactalbumin induced by the stepwise reduction of its disulfide bridges: The effect of the disulfide bridges on the structural stability of the protein in sodium dodecyl sulfate solution. J Protein Chem 14:679-684.
21. Wakselman M, Guibe-Jampel E. 1976. 1-Cyano-4-dimethylamino-pyridinium salts: New water-soluble reagents for the cyanylation of protein sulphydryl groups. J C S Chem Commun 21-22.
22. Wu J, Gage DA, Watson JT. 1996. A strategy to locate cysteine residues in proteins by specific chemical cleavage followed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. Anal Biochem 235:161-174.
23. Zaluzec EJ, Gage DA, Allison J, Watson JT. 1994. Direct MALDI mass spectrometric analysis of proteins immobilized on nylon-based membranes. J Am Soc Mass Spectrom 5:230-237.
24. Zhou Z, Smith DL. 1990. Assignment of disulfide bonds in proteins by partial acid hydrolysis and mass spectrometry. J Protein Chem 9:523-532.