Efficient lentiviral transduction of human mesenchymal stem cells that preserves proliferation and differentiation capabilities

Stem Cells Translational Medicine

Volumn 1, Issue 12, 2012, Pages 886-897

  Lin, Paul ^a   Lin, Yuan ^b   Lennon, Donald P ^a   Correa, Diego ^a   Schluchter, Mark ^c   Caplan, Arnold I ^a  

^a STANFORD UNIVERSITY   (United States)

^b STANFORD CANCER INSTITUTE   (United States)

^c CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Adult human bone marrow; Adult stem cells; Cellular therapy; Gene therapy; Lentiviral vector; Mesenchymal stem cells; Osteoblast; Transduction

Indexed keywords

FIBROBLAST GROWTH FACTOR 2; PROTAMINE SULFATE; HEPARIN ANTAGONIST; PROTAMINE;

ANIMAL CELL; ANIMAL EXPERIMENT; ARTICLE; BIOLUMINESCENCE; CELL DIFFERENTIATION; CELL ISOLATION; CELL PROLIFERATION; CENTRIFUGATION; CONTROLLED STUDY; GENE EXPRESSION; HISTOLOGY; HUMAN; HUMAN CELL; LENTIVIRINAE; MESENCHYMAL STEM CELL; MOUSE; NONHUMAN; REGULATORY SEQUENCE; TRANSGENE; VIRAL GENE DELIVERY SYSTEM; VIRUS CONCENTRATION; WESTERN BLOTTING; WOODCHUCK HEPATITIS; ANIMAL; CELL CULTURE; CYTOLOGY; DRUG EFFECT; GENETIC TRANSDUCTION; GENETICS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMA CELL; METHODOLOGY; MOUSE MUTANT; PHYSIOLOGY; WOODCHUCK HEPATITIS VIRUS;

ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; CELLS, CULTURED; FIBROBLAST GROWTH FACTOR 2; HEPARIN ANTAGONISTS; HEPATITIS B VIRUS, WOODCHUCK; HUMANS; LENTIVIRUS; MESENCHYMAL STEM CELL TRANSPLANTATION; MESENCHYMAL STROMAL CELLS; MICE; MICE, SCID; PROTAMINES; REGULATORY ELEMENTS, TRANSCRIPTIONAL; TRANSDUCTION, GENETIC; TRANSGENES;

MLCS; MLOWN;

EID: 84872910288     PISSN: 21576564     EISSN: 21576580     Source Type: Journal    
DOI: 10.5966/sctm.2012-0086     Document Type: Article

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