Human and animal mesenchymal progenitor cells from bone marrow: Identification of serum for optimal selection and proliferation

In Vitro Cellular and Developmental Biology - Animal

Volumn 32, Issue 10, 1996, Pages 602-611

  Lennon, Donald P ^a   Haynesworth, Stephen E ^a   Bruder, Scott P ^a,b   Jaiswal, Neelam ^b   Caplan, Arnold I ^a  

^a CASE WESTERN RESERVE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b OSIRIS THERAPEUTICS INC   (United States)

Author keywords

fetal bovine serum; marrow; mesenchymal stem cell; osteochondral potential; proliferation

Indexed keywords

ANIMAL CELL; ARTICLE; BONE MARROW CELL; CELL ADHESION; CELL DIFFERENTIATION; CELL GROWTH; CELL PROLIFERATION; CELL SELECTION; FIBROBLAST; HEMATOPOIETIC CELL; HUMAN; HUMAN CELL; MESENCHYME CELL; NONHUMAN; PROTEIN TRANSPORT; STEM CELL;

EID: 0030459866     PISSN: 10712690     EISSN: None     Source Type: Journal    
DOI: 10.1007/BF02724045     Document Type: Article

References (36)

1. Ashton, B. A.; Allen, T. D.; Howlett, C. R., et al. Formation of bone and cartilage by marrow stromal cells in diffusion chambers in vivo. Clinical Orthopaedics and Related Research. Clin. Orthop. 151:294-307; 1980.
2. Bab, I.; Ashton, B. A.; Gazit, D., et al. Kinetics and differentiation of marrow stromal cells in diffusion chambers in viov. J. Cell Sci. 88:139-151; 1986.
3. Barnes, D.; Sato, G. Methods for growth of cultured cells in serum-free medium. Anal. Biochem. 102:255-270; 1980.
4. Barnes, D.; Sato, G. Serum-free cell culture: a unifying approach. Cell 22:649-655; 1980.
5. Bennett, J. H.; Joyner, C. J.; Triffitt, J. T., et al. Adipocytic cells cultured from marrow have osteogenic potential. J. Cell Sci. 99:131-139; 1991.
6. Beresford. J. N. Osteogenic stem cells and the stromal system of bone and marrow. Clin. Orthop. 240:270-280; 1989.
7. Bruder, S. P.; Eames, B. F ; Haynesworth, S. E. Osteogenic induction of purified mesenchymal stem cells in vitro: quantitative assessment of the osteoblastic phenotype. Trans. Orthop. Res. Soc. 20:464; 1995.
8. Bruder, S. P.; Gazit, D.; Passi-Even, L., el al. Osteochondral differentiation and the emergence of stage-specific osteogenic cell-surface molecules by bone marrow cells in diffusion chambers. Bone Miner. 11:141-151; 1990.
9. Bruder, S. P.; Jaiswal, N. The osteogenic potential of human mesenchymal stem cells is not diminished after one-billion fold expansion in vitro. Trans. Orthop. Res. Soc.; 21:580; 1996.
10. Caplan, A. I. Effects of the nicotinamide-sensitive teratogen 3-acetylpyridine on chick limb cells in culture. Exp. Cell Res. 62:341-355; 1970.
11. Caplan, A. I. The effects of the nicotinamide-sensitive teratogen 3-acetylpyridine on chick limb mesodermal cells in culture: biochemical parameters. J. Exp. Zool. 180:351-362; 1972.
12. Caplan, A. I. Mesenchymal stem cells. J. Orthop. Res. 9:641-650; 1991.
13. Caplan, A. I.; Zwilling, E.; Kaplan, N. O. 3-Acetylpyridine: effects in vitro related to teratogenic activity in chicken embryos. Science 160:1009-1010; 1968.
14. Dennis, J. E.; Caplan, A. I. Porous ceramic vehicles for rat-marrowderived (Rattus norvegicus) osteogenic cell delivery: effects of pretreatment with fibronectin or laminin. J. Oral Implantol. 19:106-115; 1993.
15. Dennis, J. E.; Haynesworth, S. E.; Young, R. G., et al. Osteogenesis in marrow-derived mesenchymal cell porous ceramic composites transplanted subcutaneously: effect of fibronectin and laminin on cell retention and rate of osteogenic expression. Cell Transplant. 1:23-32; 1992.
16. Diduch, D. R.; Coe, M. R.; Joyner, C., et al. Two cell lines from bone marrow that differ in terms of collagen synthesis, osteogenic characteristics, and matrix mineralization. J. Bone Jt. Surg. 75A:92-105; 1993.
17. Drouet, X.; Douay, L.; Giarratana, M.-C., et al. Human liquid bone marrow culture in serum-free medium. Brit. J. Haematol. 73:143-147; 1989.
18. Friedenstein, A. J.; Chailakhjan, R. K.; Lalykina, K. S. The development of fibroblast colonies in monolayer cultures of guinea-pig bone marrow and spleen cells. Cell Tissue Kinet. 3:393-403; 1970.
19. Friedenstein, A. J.; Piatetzky-Shapiro, I. I.; Petrakova, K. V. Osteogenesis in transplants of bone marrow cells. J. Embryol. Exp. Morphol. 16:381-390; 1966.
20. Goshima, J.; Goldberg, V, M.; Caplan, A. I. The osteogenic potential of culture-expanded rat marrow mesenchymal cells assayed in vivo in calcium phosphate ceramic blocks. Clin. Orthop. 262:298-311; 1991.
21. Goshima, J.; Goldberg, V. M.; Caplan, A. I. The origin of bone formed in composite grafts of porous calcium phosphate ceramic loaded with marrow cells. Clin. Orthop. 269:274-283; 1991.
22. Haynesworth, S. E.; Goshima, J.; Goldberg, V. M., et al. Characterization of cells with osteogenic potential from human marrow. Bone 13:81-88; 1992.
23. Humason, G. Animal tissue techniques, 4th ed. San Francisco, CA: W.H. Freeman & Co.; 1979.
24. Kujawa, M. J.; Lennon, D. P.; Caplan, A. I. Growth and differentiation of stage 24 limb mesenchyme cells in a serum-free chemically defined medium. Exp. Cell Res. 183:45-61; 1989.
25. Lennon, D. P.; Haynesworth, S. E.; Young, R. G., et al. A chemically defined medium supports in vitro proliferation and maintains the osteochondral potential of rat marrow-derived mesenchymal stern cells. Exp. Cell Res. 219:211-222; 1995.
26. Morrison, R. S.; deVellis, J. Growth of purified astrocytes in a chemically defined medium. Proc. Natl. Acad. Sci. USA 78:7205-7209; 1981.
27. Nakahara, H.; Bruder, S. P.; Goldberg, V. M., et al. In vivo osteochondrogenic potential of cultured cells derived from the periosteum. Clin. Orthop. 259:223-232; 1990.
28. Nakahara, H.; Dennis, J. E.; Bruder, S. P., et al. In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells. Exp. Cell Res. 195:492-503; 1991.
29. Nakahara, H.; Goldberg, V. M.; Caplan, A. I. Culture-expanded human periosteal-derived cells exhibit osteochondral potential in vivo. J. Orthop. Res. 9:465-476; 1991.
30. Nakahara, H.; Goldberg, V. M.; Caplan, A. I. Culture-expanded periosteal-derived cells exhibit osteochondral potential in porous calcium phosphate ceramics in vivo. Clin. Orthop. 276:291-298; 1992.
31. Owen, M. Lineage of osteogenic cells and their relationship to the stromal system. In: Peck, W. A., ed. Bone and mineral research. Vol. 3. New York: Elsevier Science Publishing Co.; 1985:1-25.
32. Owen, M. Marrow stromal cells. J. Cell Sci. Suppl. 10:63-76; 1988.
33. Owen, M.; Friedenstein, A. J. Stromal stem cells: marrow-derived osteogeinc precursors. In: Evered, D.; Hainett, S., eds. Cell and molecular biology of vertebrate hard tissues. Chichester: John Wiley & Sons; 1988:42-60.
34. Rosin, A.; Freiberg, H.; Zajicek, G. The fate of rat bone marrow, spleen and periosteum cultivated in vivo in the diffusion chamber, with special reference to bone formation. Exp. Cell. Res. 29:176-187; 1963.
35. Wakitani, S.; Goto, T.; Pineda, S. J., et al. Mesenchymal cell-based repair of large, full-thickness defects of articular cartilage. J. Bone Jt. Surg. 76A:579-592; 1994.
36. Westergren-Thorsson, G.; Önnervik, P.-O.; Fransson, L.-Å., et al. Proliferation of cultured fibroblasts is inhibited by L-iduronate-containing glycosaminoglycans. J. Cell Physiol. 147:523-530; 1991.