Molecular Viability Testing of Bacterial Pathogens from a Complex Human Sample Matrix

PLoS ONE

Volumn 8, Issue 1, 2013, Pages

  Weigel, Kris M ^a   Jones, Kelly L ^a   Do, Julie S ^b   Melton Witt, Jody ^c   Chung, Jae Hyun ^d   Valcke, Christian ^c   Cangelosi, Gerard A ^a  

^a UNIVERSITY OF WASHINGTON   (United States)

^b AttoDx Inc   (United States)

^c Novartis Diagnostics   (United States)

^d University of Washington   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DRINKING WATER; GENOMIC DNA; RIBOSOME RNA;

ACINETOBACTER BAUMANNII; ARTICLE; BACTERIAL CELL; BACTERIAL GROWTH; BACTERIAL SURVIVAL; BACTERIAL VIABILITY; BACTERIAL VIRULENCE; COLONY FORMING UNIT; CONTROLLED STUDY; HUMAN; HUMAN CELL; MICROBIAL DIVERSITY; MOLECULAR PHYLOGENY; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PSEUDOMONAS AERUGINOSA; QUANTITATIVE ANALYSIS; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA ANALYSIS; STAPHYLOCOCCUS AUREUS;

ACINETOBACTER BAUMANNII; COLONY COUNT, MICROBIAL; CULTURE MEDIA; DNA, BACTERIAL; HUMANS; MICROBIAL VIABILITY; MYCOBACTERIUM TUBERCULOSIS; PSEUDOMONAS AERUGINOSA; RNA PRECURSORS; RNA, BACTERIAL; SERUM; STAPHYLOCOCCUS AUREUS;

ACINETOBACTER BAUMANNII; ACTINOBACTERIA; BACTERIA (MICROORGANISMS); FIRMICUTES; MYCOBACTERIUM TUBERCULOSIS COMPLEX; PROTEOBACTERIA; PSEUDOMONAS AERUGINOSA; STAPHYLOCOCCUS AUREUS;

EID: 84872865681     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0054886     Document Type: Article

References (29)

1. Klouche M, Schroder U, (2008) Rapid methods for diagnosis of bloodstream infections. Clinical Chemistry and Laboratory Medicine 46: 888-908.
2. Peters RP, van Agtmael MA, Danner SA, Savelkoul PH, Vandenbroucke-Grauls CM, (2004) New developments in the diagnosis of bloodstream infections. The Lancet Infectious Diseases 4: 751-760.
3. Poulsen LK, Ballard G, Stahl DA, (1993) Use of rRNA fluorescence in situ hybridization for measuring the activity of single cells in young and established biofilms. Appl Environ Microbiol 59: 1354-1360.
4. Patel BK, Banerjee DK, Butcher PD, (1993) Determination of Mycobacterium leprae viability by polymerase chain reaction amplification of 71-kDa heat-shock protein mRNA. J Infect Dis 168: 799-800.
5. Pai SR, Actor JK, Sepulveda E, Hunter RL, Jagannath C, (2000) Identification of viable and non-viable Mycobacterium tuberculosis in mouse organs by directed RT-PCR for antigen 85B mRNA. Microb Pathogenesis 28: 335-342.
6. Milner MG, Saunders JR, McCarthy AJ, (2001) Relationship between nucleic acid ratios and growth in Listeria monocytogenes. Microbiology 147: 2689-2696.
7. Mdivani N, Li H, Akhalaia M, Gegia M, Goginashvili L, et al. (2009) Monitoring Therapeutic Efficacy by Real-Time Detection of Mycobacterium tuberculosis mRNA in Sputum. Clin Chem 55: 1694-1700.
8. Li L, Mahan CS, Palaci M, Horter L, Loeffelholz L, et al. (2010) Sputum Mycobacterium tuberculosis mRNA as a Marker of Bacteriologic Clearance in Response to Antituberculosis Therapy. J Clin Microbiol 48: 46-51.
9. Kobayashi H, Oethinger M, Tuohy MJ, Procop GW, Hall GS, et al. (2009) Limiting false-positive polymerase chain reaction results: detection of DNA and mRNA to differentiate viable from dead bacteria. Diagnostic Microbiology and Infectious Disease 64: 445-447.
10. Gonzalez-Escalona N, Hammack TS, Russell M, Jacobson AP, De Jesus AJ, et al. (2009) Detection of Live Salmonella sp. Cells in Produce by a TaqMan-Based Quantitative Reverse Transcriptase Real-Time PCR Targeting invA mRNA. Appl Environ Microbiol 75: 3714-3720.
11. Liu Y, Wang C, Fung C, Li XF, (2010) Quantification of Viable but Nonculturable Escherichia coli O157: H7 by Targeting the rpoS mRNA. Anal Chem 82: 2612-2615.
12. Nocker A, Camper AK, (2009) Novel approaches toward preferential detection of viable cells using nucleic acid amplification techniques. FEMS Microbiol Lett 291: 137-142.
13. Kobayashi H, Oethinger M, Tuohy MJ, Hall GS, Bauer TW, (2010) Distinction between intact and antibiotic-inactivated bacteria by real-time PCR after treatment with propidium monoazide. J Orthop Res 28: 1245-1251.
14. Nocker A, Sossa KE, Camper AK, (2007) Molecular monitoring of disinfection efficacy using propidium monoazide in combination with quantitative PCR. Journal of Microbiological Methods 70: 252-260.
15. Gedalanga P, Olson B, (2009) Development of a quantitative PCR method to differentiate between viable and nonviable bacteria in environmental water samples. Applied Microbiology and Biotechnology 82: 587-596.
16. Flekna G, Stefani P, Wagner M, Smulders FJM, Mozina SS, et al. (2007) Insufficient differentiation of live and dead Campylobacter jejuni and Listeria monocytogenes cells by ethidium monoazide (EMA) compromises EMA/real-time PCR. Research in Microbiology 158: 405-412.
17. Kobayashi H, Oethinger M, Tuohy MJ, Hall GS, Bauer TW, (2009) Unsuitable distinction between viable and dead Staphylococcus aureus and Staphylococcus epidermidis by ethidium bromide monoazide. Letters in Applied Microbiology 48: 633-638.
18. Cangelosi GA, Weigel KM, Lefthand-Begay C, Meschke JS, (2010) Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis. Appl Environ Microbiol 76: 960-962.
19. Oerther DB, Pernthaler J, Schramm A, Amann R, Raskin L, (2000) Monitoring Precursor 16S rRNAs of Acinetobacter spp. in Activated Sludge Wastewater Treatment Systems. Appl Environ Microbiol 66: 2154-2165.
20. Cangelosi GA, Brabant WH, (1997) Depletion of pre-16S rRNA in starved Escherichia coli cells. J Bacteriol 179: 4457-4463.
21. Mackow ER, Chang FN, (1985) Processing of precursor ribosomal RNA and the presence of a modified ribosome assembly scheme in Escherichia coli relaxed strain. FEBS Letters 182: 407-412.
22. Srivastava AK, Schlessinger D, (1990) Mechanism and Regulation of Bacterial Ribosomal RNA Processing. Annu Rev Microbiol 44: 105-129.
23. Lu T, Stroot PG, Oerther DB, (2009) Reverse Transcription of 16S rRNA To Monitor Ribosome-Synthesizing Bacterial Populations in the Environment. Appl Environ Microbiol 75: 4589-4598.
24. Oerther DB, van Loosdrecht MC, (2002) Raskin (2002) Quantifying the impact of wastewater micronutrient composition on in situ growth activity of Acinetobacter spp. Water Sci Technol 46: 443-437.
25. Britton RA, Wen T, Schaefer L, Pellegrini O, Uicker WC, et al. (2007) Maturation of the 5′ end of Bacillus subtilis 16S rRNA by the essential ribonuclease YkqC/RNase J1. Molecular Microbiology 63: 127-138.
26. Condon C, Brechemier-Baey D, Beltchev B, Grunberg-Manago M, Putzer H, (2001) Identification of the gene encoding the 5S ribosomal RNA maturase in Bacillus subtilis: mature 5S rRNA is dispensable for ribosome function. RNA 7: 242-253.
27. Redko Y, Bechhofer DH, Condon C, (2008) Mini-III, an unusual member of the RNase III family of enzymes, catalyses 23S ribosomal RNA maturation in B.-ásubtilis. Molecular Microbiology 68: 1096-1106.
28. Stroot PG, Oerther DB, (2003) Elevated precursor 16S rRNA levels suggest the presence of growth inhibitors in wastewater. Water Sci Technol 47: 241-250.
29. Nanassy OZ, Haydock P, Beck N, Barker LM, Hargrave P, et al. (2011) Extraction of MS2 Phage RNA from Upper Respiratory Tract Specimens by Use of Flat Glass Devices. J Clin Microbiol 49: 1010-1016.