Detection of live Salmonella sp. cells in produce by a taqman-based quantitative reverse transcriptase real-time PCR Targeting invA mRNA

Applied and Environmental Microbiology

Volumn 75, Issue 11, 2009, Pages 3714-3720

  González Escalona, Narjol ^a   Hammack, Thomas S ^a   Russell, Mindi ^a   Jacobson, Andrew P ^a   De Jesús, Antonio J ^a   Brown, Eric W ^a   Lampel, Keith A ^a  

^a CENTER FOR DRUG EVALUATION AND RESEARCH   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CULTURE METHODS; DEAD CELLS; DETECTION LIMITS; DNA DETECTION; FALSE NEGATIVES; INTERNAL CONTROLS; JACKSON; MRNA LEVEL; PUBLIC HEALTH; Q-RT-PCR; REAL TIME RT-PCR; REAL-TIME PCR; REVERSE TRANSCRIPTASE; SALMONELLA ENTERICA; SALMONELLA SPP;

CELL MEMBRANES; FRUITS; MARKUP LANGUAGES; NUCLEIC ACIDS; RNA;

CELL CULTURE;

BACTERIAL RNA; MESSENGER RNA;

BACTERIUM; DETECTION METHOD; ENZYME ACTIVITY; POLYMERASE CHAIN REACTION; PUBLIC HEALTH; QUANTITATIVE ANALYSIS; REAL TIME; RNA;

ACCURACY; ARTICLE; BACTERIUM CULTURE; BACTERIUM DETECTION; COLONY FORMING UNIT; CONTROLLED STUDY; FOOD ANALYSIS; FOOD CONTAMINATION; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPPER; QUANTITATIVE ASSAY; REAL TIME POLYMERASE CHAIN REACTION; RELIABILITY; REPRODUCIBILITY; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SALMONELLA; SALMONELLA ENTERICA; SEROTYPE; SPINACH; TECHNIQUE; TOMATO;

BACTERIAL PROTEINS; DNA, BACTERIAL; MOLECULAR SEQUENCE DATA; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SALMONELLA; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS, DNA; VEGETABLES;

CAPSICUM; LYCOPERSICON ESCULENTUM; SALMONELLA; SALMONELLA ENTERICA; SALMONELLA SP.; SPINACIA OLERACEA;

EID: 66249088328     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02686-08     Document Type: Article

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