Extraction of MS2 phage RNA from upper respiratory tract specimens by use of flat glass devices

Journal of Clinical Microbiology

Volumn 49, Issue 3, 2011, Pages 1010-1016

  Nanassy, Oliver Z ^a   Haydock, Paul ^a   Beck, Nicola ^b   Barker, Lynn M ^a   Hargrave, Perry ^a   Gestwick, Daniel ^a   Lindsey, Wesley C ^c   Reed, Michael W ^a   Meschke, J Scott ^b  

^a Blood Cell Storage   (United States)

^b UNIVERSITY OF WASHINGTON   (United States)

^c Nanosphere Inc   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLASS; VIRUS RNA;

ARTICLE; AUTOMATION; CONTROLLED STUDY; INTERMETHOD COMPARISON; MS2 VIRUS; NONHUMAN; NOSE SMEAR; PRIORITY JOURNAL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA EXTRACTION; RNA PURIFICATION; SENSITIVITY ANALYSIS; UPPER RESPIRATORY TRACT;

AUTOMATION; BODILY SECRETIONS; EQUIPMENT AND SUPPLIES; GLASS; HUMANS; LEVIVIRUS; RESPIRATORY SYSTEM; RNA, VIRAL; SENSITIVITY AND SPECIFICITY; VIROLOGY;

EID: 79952353946     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01132-10     Document Type: Article

References (19)

1. Abu Al-Soud, W., and P. Radstrom. 2000. Effects of amplification facilitators on diagnostic PCR in the presence of blood, feces, and meat. J. Clin. Microbiol. 38:4463-4470.
2. Adams, M. H. 1959. Bacteriophages. Interscience, New York, NY.
3. Beck, N. K., et al. 2010. Abstr. 110th Gen. Meet. Am. Soc. Microbiol., poster Q-431.
4. Boom, R., C. J. Sol, R. Heijtink, P. M. Wertheim-van Dillen, and J. van der Noordaa. 1991. Rapid purification of hepatitis B virus DNA from serum. J. Clin. Microbiol. 29:1804-1811.
5. Boom, R., et al. 1990. Rapid and simple method for purification of nucleic acids. J. Clin. Microbiol. 28:495-503.
6. Bustin, S. A., et al. 2009. The MIQE guidelines: minimum information for publication of quantitative real-time PCR experiments. Clin. Chem. 55:611-622.
7. Chan, K. H., et al. 2008. Comparison of the NucliSens easyMAG and Qiagen BioRobot 9604 nucleic acid extraction systems for detection of RNA and DNA respiratory viruses in nasopharyngeal aspirate samples. J. Clin. Microbiol. 46:2195-2199.
8. Dundas, N., N. K. Leos, M. Mitui, P. Revell, and B. B. Rogers. 2008. Comparison of automated nucleic acid extraction methods with manual extraction. J. Mol. Diagn. 10:311-316.
9. Fleischhacker, M., and B. Schmidt. 2007. Circulating nucleic acids (CNAs) and cancer - a survey. Biochim. Biophys. Acta 1775:181-232.
10. Jones, L. J., S. T. Yue, C. Y. Cheung, and V. L. Singer. 1998. RNA quantitation by fluorescence-based solution assay: RiboGreen reagent characterization. Anal. Biochem. 265:368-374.
11. Kirchgesser, M., et al. 2009. Maximizing recovery of viral DNA/RNA isolation - what are the critical parameters for an optimized process? J. Mol. Diagn. 11:672.
12. Nanassy, O. Z., P. V. Haydock, and M. W. Reed. 2007. Capture of genomic DNA on glass microscope slides. Anal. Biochem. 365:240-245.
13. O'Connell, K. P., et al. 2006. Real-time fluorogenic reverse transcription-PCR assays for detection of bacteriophage MS2. Appl. Environ. Microbiol. 72:478-483.
14. Read, S. J. 2001. Recovery efficiencies on nucleic acid extraction kits as measured by quantitative LightCycler PCR. Mol. Pathol. 54:86-90.
15. Reed, M. W., et al. October 2009. Microfluidic devices for extraction and analysis of nucleic acids from biological samples. U.S. patent 7,608,399.
16. Schmidt, B., S. Weickmann, C. Witt, and M. Fleischhacker. 2005. Improved method for isolating cell-free DNA. Clin. Chem. 51:1561-1563.
17. Stahlberg, A., J. Hakansson, X. Xian, H. Semb, and M. Kubista. 2004. Properties of the reverse transcription reaction in mRNA quantification. Clin. Chem. 50:509-515.
18. Vogelstein, B., and D. Gillespie. 1979. Preparative and analytical purification of DNA from agarose. Proc. Natl. Acad. Sci. U. S. A. 76:615-619.
19. Wen, J., L. A. Legendre, J. M. Bienvenue, and J. P. Landers. 2008. Purification of nucleic acids in microfluidic devices. Anal. Chem. 80:6472-6479.